纤维素酶R-10 | 9012-54-8

• 物质功能分类: 食品添加剂 - 酶制剂
• 分子结构分类: 有机化合物 - 有机氧化合物
• 中文名称: 纤维素酶R-10
• 中文别名: 果胶酶；Cytochrome鎐frompigeonbreastmuscle；离析酶R-10；纤维素酶；蜗牛酶；Cytochrome鎐equineheart；纤维素酶(来自黑曲霉)；纤维素酶来源于里氏木霉ATCC26921
• 英文名称: cellotriose
• 英文别名: Cellulase；(2S,3R,4S,5S,6R)-2-[(2R,3S,4R,5R,6S)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2R,3S,4R,5R,6R)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol
• CAS: 9012-54-8
• 化学式: C₁₈H₃₂O₁₆
• 分子量: 504.442
• InChiKey: FYGDTMLNYKFZSV-CSHPIKHBSA-N

物化性质

• 密度：
  1.2 g/mL at 25 °C
• 溶解度：
  在去离子水中的溶解度为5.0mg/mL（无菌；存在0.15%聚六亚甲基双胍(PHMB)。）
• LogP：
  -1.3 at 20℃
• 稳定性/保质期：

  Other notes: For more information on enzymes for complex carbohydrate analysis, visit www.sigma-aldrich.com/enzymeexplorer.

  Unit definition: One unit will liberate 1.0 μmole of glucose from cellulose in one hour at pH 5.0 at 37 °C (2-hour incubation time).

计算性质

• 辛醇/水分配系数(LogP)：
  -6.9
• 重原子数：
  34
• 可旋转键数：
  7
• 环数：
  3.0
• sp3杂化的碳原子比例：
  1.0
• 拓扑面积：
  269
• 氢给体数：
  11
• 氢受体数：
  16

ADMET

毒理性

• 副作用

哮喘 - 由吸入刺激性或过敏原物质引发的 可逆性支气管收缩（支气管小管狭窄）。

Asthma - Reversible bronchoconstriction (narrowing of bronchioles) initiated by the inhalation of irritating or allergenic agents.

来源:Haz-Map, Information on Hazardous Chemicals and Occupational Diseases

安全信息

• TSCA：
  Yes
• 危险品标志：
  Xn,Xi
• 安全说明：
  S2,S22,S23,S24,S36/37,S45
• 危险类别码：
  R42
• WGK Germany：
  1
• 海关编码：
  35079090
• 储存条件：
  2-8°C下应密闭保存，并置于阴凉干燥处。

制备方法与用途

纤维素酶是一种复合酶，通常由三种主要组分构成：C1酶、CX酶以及葡萄糖苷酶。这些组分协同作用下能够分解纤维素分子成低聚纤维素，并最终水解为葡萄糖。

1. C1酶 - 通常被认为具有内切活性，负责在纤维素大分子内部切割β-1,4-糖苷键。
2. CX酶 - 被认为是外切酶，它从纤维素大分子末端开始逐步切开β-1,4-糖苷键。
3. 葡萄糖苷酶 - 作为最终的组分参与作用，将生成的纤维二糖进一步水解为葡萄糖。

这种协同工作模式使得纤维素酶能够高效地分解复杂的纤维素结构。值得注意的是，在实际应用中，为了提高酶的作用效率，通常需要预先对纤维素进行溶胀处理，以增加其可接近性及活性部位的数量。

这些酶不仅在工业上有广泛的应用（如食品、纺织、造纸等），还在医药领域用于改善消化不良等问题，并且作为饲料添加剂可以提高动物对植物性食物中难以被消化的成分的利用率。

反应信息

• 作为反应物：
  描述：

  纤维素酶R-10 在 2-氯-1,3-二甲基氯化咪唑啉 、 三乙胺 作用下， 以 水 为溶剂， 反应 0.25h， 以100%的产率得到1,6-anhydro-β-D-cellotriose
  参考文献：
  名称：

  Direct synthesis of 1,6-anhydro sugars from unprotected glycopyranoses by using 2-chloro-1,3-dimethylimidazolinium chloride

  摘要：

  Various 1,6-anhydro sugars have been synthesized directly from the corresponding unprotected glycopyranoses in excellent yields by using 2-chloro-1,3-dimethylimidazolinium chloride (DMC) as a dehydrative condensing agent. The reactions took place smoothly under mild reaction conditions in aqueous media. The present method would be a practical tool for synthesis of 1,6-anhydro derivatives of mono-saccharides, linear-oligosaccharides, and branched-oligosaccharides. (C) 2009 Elsevier Ltd. All rights reserved.

  DOI：

  10.1016/j.tetlet.2009.02.171
• 作为产物：
  描述：

  cellulose 在 cellulase from Trichoderma reesei 、 水 作用下， 以 aq. buffer 为溶剂， 反应 72.0h， 生成 葡萄糖 、 纤维素酶R-10 、 Cellobiose
  参考文献：
  名称：

  Analysis of mono- and oligosaccharides in ionic liquid containing matrices

  摘要：

  Ionic liquids (ILs), that is, salts with melting points <100 degrees C, have recently attracted a lot of attention in biomass processing due to their ability to dissolve lignocellulosics. In this work, we studied how two imidazolium-based, hydrophilic, cellulose dissolving ionic liquids 1,3-dimethylimidazolium dimethylphosphate [DMIM]DMP and 1-ethyl-3-methylimidazolium acetate [EMIM]AcO affect the usually employed analytical methods for mono- and oligosaccharides, typical products from hydrolytic treatments of biomass. HPLC methods were severely hampered by the presence of ILs with loss of separation power and severe baseline problems, making their use for saccharide quantification extremely challenging. Problems in DNS photometric assay and chromatography were also encountered at high ionic liquid concentrations and many capillary electrophoresis (CE) methods did not allow an efficient analysis of saccharides in these matrices. In this paper we describe an optimized CE method with pre-column derivatization for the qualitative and quantitative analysis of mono- and oligosaccharides in sample matrices containing moderate (20-40% (v/v)) concentrations of ILs. The IL content and type in the sample matrix was found to affect both peak shape and quantification parameters. Generally, the presence of high IL concentrations (>= 20% (v/v)) had a dampening effect on the detection of the analytes. IL in lower concentrations of <20% (v/v) was, however, found to improve peak shape and/or separation in some cases. The optimized CE method has good sensitivity in moderate concentrations of the ionic liquids used, with limits of detection of 5 mg/L for cellooligomers up to the size of cellotetraose and 5-20 mg/L for cellopentaose and cellohexaose, depending on the matrix. The method was used for analysing the action of a commercial beta-glucosidase in ILs and for analysing saccharides in the IL containing hydrolysates from the hydrolysis of microcrystalline cellulose with Trichoderma reesei endoglucanase Cel5A. According to the results, [DMIM]DMP and [EMIM]AcO] showed clear differences in enzyme inactivation. (C) 2012 Elsevier Ltd. All rights reserved.

  DOI：

  10.1016/j.carres.2012.11.006

文献信息

• CHARACTERIZATION OF MONO- AND OLIGOSACCHARIDES PRODUCED BY CO₂LASER IRRADIATION ON CELLULOSE
  作者：Jun Suzuki、Jun-ichi Azuma、Tetsuo Koshijima、Keizo Okamura、Hajime Okamoto

  DOI：10.1246/cl.1983.481

  日期：1983.4.5

  The chemical structures of three mono-, two di-, and two trisaccharides (1∼7) isolated from the pyrolysis products formed by CO2 laser irradiation on cellulose were investigated.

  研究了从二氧化碳激光辐照纤维素产生的热解产物中分离出的三种单糖、两种二糖和两种三糖（1∼7）的化学结构。
• Mass Spectrometric Studies of Fast Pyrolysis of Cellulose
  作者：John C. Degenstein、Matt Hurt、Priya Murria、McKay Easton、Harshavardhan Choudhari、Linan Yang、James Riedeman、Mark S. Carlsen、John J. Nash、Rakesh Agrawal、W. Nicholas Delgass、Fabio H. Ribeiro、Hilkka I. Kenttämaa

  DOI：10.1255/ejms.1335

  日期：2015.6

  A fast pyrolysis probe/linear quadrupole ion trap mass spectrometer combination was used to study the primary fast pyrolysis products (those that first leave the hot pyrolysis surface) of cellulose, cellobiose, cellotriose, cellotetraose, cellopentaose, and cellohexaose, as well as of cellobiosan, cellotriosan, and cellopentosan, at 600°C. Similar products with different branching ratios were found

  使用快速热解探针/线性四极杆离子阱质谱仪组合来研究纤维素、纤维二糖、纤维三糖、纤维四糖、纤维五糖和纤维六糖以及纤维二糖的主要快速热解产物（那些首先离开热解表面的产物） 、纤维三聚糖和纤维戊聚糖，在 600°C。如前所述，在低聚糖和纤维素中发现了具有不同支化率的类似产品。然而，对于纤维三聚糖（和纤维戊聚糖）和纤维素，测量了具有相似支化率的相同产品（除了两个）。该结果表明，纤维三聚糖是研究纤维素快速热解的极好小分子替代物，并且纤维素的大多数快速热解产物并非来自还原端。
• Mass Spectrometric Analysis Using Ruthenium (II)-Labeling for Identification of Glycosyl Hydrolase Product
  作者：Akihiro ITO、Taka-aki OKAMURA、Koichi UEGAKI、Han-Woo KIM、Kazuhiko ISHIKAWA、Tsutomu NAKAMURA、Hitoshi YAMAMOTO、Norikazu UEYAMA

  DOI：10.1271/bbb.80492

  日期：2009.2.23

  Analysis of products digested by glycosyl hydrolases helps understanding of the hydrolysis mechanism and the substrate recognition in the enzymes. We developed a new universal technique, which consists of ruthenium (II) complex labeling and mass spectrometry analysis, to identify the reducing sugars released from oligosaccharides by enzymatic digestion. This method was applied to enzymatic digestion

  分析糖基水解酶消化的产物有助于理解酶的水解机理和底物识别。我们开发了一种新的通用技术，该技术由钌（II）复杂标记和质谱分析组成，以鉴定通过酶消化从寡糖释放的还原糖。该方法分别应用于过嗜热古细菌火球菌和and火球菌的几丁质酶和纤维素酶的酶消化。
• Hydrolysis behaviors of sugarcane bagasse pith in subcritical carbon dioxide–water
  作者：Jiezhen Liang、Xiaopeng Chen、Linlin Wang、Xiaojie Wei、Feifei Qiu、Chaochao Lu

  DOI：10.1039/c6ra18436g

  日期：——

  temperatures (160 to 260 °C), liquid to solid ratios (20 : 1 to 100 : 1), CO2 pressures (0 to 7.3 MPa), stirring speeds (0 to 500 rpm) and reaction times (0 to 40 min). The highest total reducing sugar yield (43.6%) was obtained at 200 °C, liquid to solid ratio 30 : 1, 2 MPa CO2, 500 rpm and 50 min. Two-dimensional heteronuclear single quantum coherence (2D HSQC) nuclear magnetic resonance (NMR), scanning electron

  这项研究的目的是描述蔗糖甘蔗渣髓（SCBP）在亚临界CO 2水中的水解行为。在间歇反应器中使用不同的温度（160至260°C），液固比（20：1至100：1），CO 2压力（0至7.3 MPa），搅拌速度（0至500）进行水解rpm）和反应时间（0到40分钟）。在200°C，液固比30：1、2 MPa CO 2下获得最高的总还原糖收率（43.6％），500 rpm和50分钟。使用二维异核单量子相干（2D HSQC）核磁共振（NMR），扫描电子显微镜（SEM）和傅里叶变换红外光谱（FT-IR）来帮助阐明原料和残留物的物理和化学特性固体颗粒，其结果与水解过程中半纤维素的去除一致。分析了产品浓度随时间的变化，以通过高效液相色谱（HPLC）了解产品分布并推断反应机理。