Rattan tea regarded as one of the most promising dietary supplements for the prevention and treatment of many chronic diseases has gained extensive attention. Free fatty acids play an important role in human body as essential nutrients. Contents of 31 free fatty acids in Rattan Tea were firstly investigated using toluene-4-sulfonic acid 2-(naphthalene-1-yl-phenyl-amino)-ethyl ester as pre-column fluorescence derivatization reagent by RE-HPLC coupled with an atmospheric chemical ionization-mass spectrometry source (APCI/MS). The results indicated that lower values of LOD (0.52-1.53 ng mL-1) provided the guarantee for trace analysis and evaluation of free fatty acids. The experimental recoveries were in the range of 88.9-103.6 %. Results of the present study indicated the highest free fatty acids content of Rattan Tea was in April. Thirteen free fatty acids were found, including C18:2w6, C18:3w3, w6 and C20:4w6 essential to life. The developed method exhibited powerful potential for the systematic detection of bioactive components of medicinal plants.
藤椰茶作为预防和治疗许多慢性疾病的最有前景的膳食补充剂之一,受到广泛关注。游离
脂肪酸在人体中作为必需营养素起着重要作用。首次使用
对甲苯磺酸2-(
萘-1-基-苯
氨基)-乙基酯作为前柱荧光
衍生化试剂,利用高效
液相色谱-大气
化学电离质谱(APCI/MS)技术,研究了藤椰茶中31种游离
脂肪酸的含量。结果表明,低的检出限(0.52-1.53 ng mL-1)为游离
脂肪酸的微量分析和评估提供了保障。实验的回收率在88.9%-103.6%之间。本研究的结果显示,藤椰茶中游离
脂肪酸含量最高的月份是四月。共发现了13种游离
脂肪酸,其中包括生命必需的C18:2w6、C18:3w3、w6和C20:4w6。所开发的方法展示了系统检测药用植物
生物活性成分的强大潜力。