Soyasaponin I | 51330-27-9

• 物质功能分类: 生物化工 - 中草药成分
• 分子结构分类: 有机化合物 - 脂质和类脂质分子 - 异戊烯醇脂质
• 英文名称: Soyasaponin I
• 英文别名: soyasaponin I；(2S,3S,4S,5R,6R)-6-[[(3S,4S,6aR,6bS,8aR,9R,14bR)-9-hydroxy-4-(hydroxymethyl)-4,6a,6b,8a,11,11,14b-heptamethyl-1,2,3,4a,5,6,7,8,9,10,12,12a,14,14a-tetradecahydropicen-3-yl]oxy]-5-[(2S,3R,4S,5R,6R)-4,5-dihydroxy-6-(hydroxymethyl)-3-[(2S,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methyloxan-2-yl]oxyoxan-2-yl]oxy-3,4-dihydroxyoxane-2-carboxylic acid
• CAS: 51330-27-9；82793-05-3
• 化学式: C₄₈H₇₈O₁₈
• 分子量: 943.137
• InChiKey: PTDAHAWQAGSZDD-PYEALAKRSA-N

物化性质

• 熔点：
  220-222 °C(Solv: methanol (67-56-1))
• 沸点：
  1024.2±65.0 °C(Predicted)
• 密度：
  1.41
• 溶解度：
  在乙醇中的溶解度为1mg/mL，透明，无色

计算性质

• 辛醇/水分配系数(LogP)：
  2.4
• 重原子数：
  66
• 可旋转键数：
  9
• 环数：
  8.0
• sp3杂化的碳原子比例：
  0.94
• 拓扑面积：
  295
• 氢给体数：
  11
• 氢受体数：
  18

安全信息

• WGK Germany：
  3
• 海关编码：
  29389090

制备方法与用途

性质

大豆皂苷Bb可溶于水、易溶于热乙醇，但难溶于乙醚和苯等极性较小的有机溶剂。

药理作用

大豆皂苷Bb是从豆科植物大豆（Glycine max (L.) Merr.）种子中提取的，具有降低胆固醇、降血脂及降血糖的作用，并且还具备抗氧化功能。

用途

大豆皂苷Bb主要用于含量测定、鉴定和药理实验等。

生物活性

soyasaponin Bb 是从菜豆科植物中分离出的一种大豆皂苷，能够作为醛糖还原酶差异抑制剂 (ARDI)。

反应信息

• 作为反应物：
  描述：

  Soyasaponin I 生成 α-L-rhamnopyranosyl-(1->2)-β-D-galactopyranosyl-(1->2)-β-D-glucuronopyranose
  参考文献：
  名称：

  Purification and Some Properties of Soybean Saponin Hydrolase fromAspergillus oryzaeKO-2

  摘要：

  We had investigated the enzymatic hydrolysis of soybean saponins and selected soybean saponin hydrolase from Aspergillus oryzae KO-2. We attempted purification of this enzyme for further characterization. This enzyme was purified 1500-fold using ammonium sulfate fractionation and Sephadex G-200 gel filtrations. The enzyme was electrophoretically homogeneous and a glycoprotein by PAS staining. By gel filtration, the molecular weight of enzyme was 158,000 and SDS-PAGE showed the enzyme to have a tetrameric structure composed of heterogeneous subunits of 35,000 and 45,000 The enzyme activity was stable at temperatures below 40°C and stable from pH 5.0 to 8.0. The optimum pH was pH 4.5 to 5.0 and the optimum temperature was 50°C. The Km and Vmax for soyasaponin I were 0.48 mm and 9.8 μmol/hr mg protein, respectively. After hydrolysis with the enzyme, soyasapogenol B and α-l-rhamnopyranosyl (1 → 2)-β-d-galactopyranosyl (1 → 2)-d-glucuronopyrano-side were released from soyasaponin I.

  DOI：

  10.1080/00021369.1991.10870531

文献信息

• Purification and Some Properties of Soybean Saponin Hydrolase from<i>Aspergillus oryzae</i>KO-2
  作者：Shigemitsu Kudou、Iwao Tsuizaki、Teiji Uchida、Kazuyoshi Okubo

  DOI：10.1080/00021369.1991.10870531

  日期：1991.1

  We had investigated the enzymatic hydrolysis of soybean saponins and selected soybean saponin hydrolase from Aspergillus oryzae KO-2. We attempted purification of this enzyme for further characterization. This enzyme was purified 1500-fold using ammonium sulfate fractionation and Sephadex G-200 gel filtrations. The enzyme was electrophoretically homogeneous and a glycoprotein by PAS staining. By gel filtration, the molecular weight of enzyme was 158,000 and SDS-PAGE showed the enzyme to have a tetrameric structure composed of heterogeneous subunits of 35,000 and 45,000 The enzyme activity was stable at temperatures below 40°C and stable from pH 5.0 to 8.0. The optimum pH was pH 4.5 to 5.0 and the optimum temperature was 50°C. The Km and Vmax for soyasaponin I were 0.48 mm and 9.8 μmol/hr mg protein, respectively. After hydrolysis with the enzyme, soyasapogenol B and α-l-rhamnopyranosyl (1 → 2)-β-d-galactopyranosyl (1 → 2)-d-glucuronopyrano-side were released from soyasaponin I.