2-乙酰氨基-2-脱氧-d-葡萄糖酸内酯 | 19026-22-3

• 物质功能分类: 有机原料 - 羧酸类化合物及衍生物
• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 羧酸及其衍生物
• 中文名称: 2-乙酰氨基-2-脱氧-d-葡萄糖酸内酯
• 中文别名: 2-乙酰氨基-2-脱氧-3-D葡糖酸-1,5-内酯
• 英文名称: 2-Acetamido-2-deoxy-D-glucono-1,5-lactone
• 英文别名: N-acetyl-D-glucosamino-1,5-lactone；N-[(3R,4R,5S,6R)-4,5-dihydroxy-6-(hydroxymethyl)-2-oxooxan-3-yl]acetamide
• CAS: 19026-22-3
• 化学式: C₈H₁₃NO₆
• 分子量: 219.194
• InChiKey: NELQYZRSPDCGRQ-DBRKOABJSA-N

物化性质

• 熔点：
  168-170°C
• 沸点：
  602.0±55.0 °C(Predicted)
• 密度：
  1.50±0.1 g/cm3(Predicted)
• 溶解度：
  可溶于DMSO（轻微）、甲醇（轻微）、水（轻微、超声处理）

计算性质

• 辛醇/水分配系数(LogP)：
  -2
• 重原子数：
  15
• 可旋转键数：
  2
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.75
• 拓扑面积：
  116
• 氢给体数：
  4
• 氢受体数：
  6

反应信息

• 作为反应物：
  描述：

  水 、 2-乙酰氨基-2-脱氧-d-葡萄糖酸内酯 生成 氢(+1)阳离子 、 N-Acetyl-D-glucosaminate
  参考文献：
  名称：

  摘要：

  DOI：
• 作为产物：
  描述：

  2-乙酰氨基-2-脱氧-beta-D-吡喃葡萄糖胺 在 N-acetyl-D-hexosamine oxidase (EC 1.1.3.29) 作用下， 以 aq. phosphate buffer 为溶剂， 反应 1.0h， 生成 2-乙酰氨基-2-脱氧-d-葡萄糖酸内酯
  参考文献：
  名称：

  使用高通量活性筛选发现两种新型氧化酶

  摘要：

  寻找新的酶活性：高通量活性筛选平台的应用导致了两种新酶的发现：N-乙酰己糖胺氧化酶（一种以前的孤儿酶）和一种新型长链醇氧化酶（一种与具有此活性的已知酶的非同源同功能酶（NISE）。

  DOI：

  10.1002/cbic.202100510

文献信息

• 2-acylamino-2-deoxy-glucono-1,5-lactones, a method for the production thereof, compositions containing them, and uses thereof
  申请人：——

  公开号：US20020028954A1

  公开（公告）日：2002-03-07

  The invention concerns compounds of formula (I) wherein: A represents R 1 or (O)R 1 where R 1 represents an alkyl group comprising 1 to 30 carbon atoms, linear or branched, saturated or unsaturated, capable of being partly or totally substituted by Hal where Hal represents —Cl, —Br, or —F, and of being interrupted by one or several units selected among —O—, —S—, —C(O)—, —NR 3 C(O)—, —Ph(R 4 ) n and —CH 2 —CH 2 —O) n —, wherein R 3 represents or —CH 2 ) n″ —CH where n″=0 to 17; R 4 represents, —CH 3 , —CH 2 H 5 , —C 3 H 7 and n=0 to 4 and n′=1, 2 or 3, or R 1 represents a cyclanic radical with diterpene or triterpene root; and R 2 represents a C 1 -C 11 linear or branched alkyl group. The invention also concerns a method for obtaining said compounds, compositions containing them and their use as surfactant, and in enzymatic processes using a chitinase, in particular N-acetylglucosaminidase.

  该发明涉及以下式(I)的化合物：其中：A代表R1或（O）R1，其中R1代表由1至30个碳原子组成的烷基基团，直链或支链，饱和或不饱和，可以部分或完全被Hal取代，其中Hal代表-Cl，-Br或-F，并且可以被选自-O-，-S-，-C(O)-，-NR3C(O)-，-Ph(R4)n和-CH2-CH2-O)n-的一个或多个单位中断，其中R3代表或-CH2)n″-CH2，其中n″=0至17；R4代表-CH3，-CH2H5，-C3H7和n=0至4，n′=1，2或3，或者R1代表带有二萜或三萜基团的环烷基基团；R2代表C1-C11直链或支链烷基基团。该发明还涉及一种获得所述化合物的方法，含有它们的组合物以及它们作为表面活性剂的用途，以及在使用壳聚糖酶，特别是N-乙酰葡萄糖氨基酶的酶促过程中的用途。
• Advanced drug development and manufacturing
  申请人：Los Alamos National Security, LLC

  公开号：EP2511844A2

  公开（公告）日：2012-10-17

  There is described an apparatus for measuring protein characteristics comprising an X-ray fluorescence (XRF) spectrometer comprising a source of polychromatic X-rays, an X-ray detector, a protein, a molecule that has been exposed to and at least weakly binds to the protein, a plurality of X-ray fluorescence signal data obtained by irradiating chemical elements in the protein and molecule with the polychromatic X-rays and a security system for maintaining records for the data from the plurality of X-ray fluorescence signal measurements. There is also described an x-ray microscope for measuring a sample.

  描述了一种测量蛋白质特性的仪器，该仪器包括一个 X 射线荧光 (XRF) 光谱仪，其中包括一个多色 X 射线源、一个 X 射线探测器、一个蛋白质、一个已暴露于该蛋白质并至少与该蛋白质弱结合的分子、通过用多色 X 射线照射蛋白质和分子中的化学元素而获得的多个 X 射线荧光信号数据，以及一个用于维护多个 X 射线荧光信号测量数据记录的安全系统。此外，还介绍了一种用于测量样品的 X 射线显微镜。
• UDP-N-acetylglucosamine: Galactose-beta1,3-N-acetylgalactosamine-alpha-R / N-acetylglucosamine-beta1,3,-N-acetylgalactosamine-alpha-R (GlcNAc to GalNAc) beta1,6-N-acetylglucosaminyltransferase, C2/4GnT
  申请人：——

  公开号：US20020081656A1

  公开（公告）日：2002-06-27

  A novel gene defining a novel human UDP-GlcNAc: Gal/Gl cNAc&bgr; 1-3GalNAc a&bgr;1, 6GlcNAc-transferase, termed C2/4GnT, with unique enzymatic properties is disclosed. The enzymatic activity of C2/4GnT is shown to be distinct from that of previously identified enzymes of this gene family. The invention discloses isolated DNA molecules and DNA constructs encoding C2/4GnT and derivatives thereof by way of amino acid deletion, substitution or insertion exhibiting C2/4GnT activity, as well as cloning and expression vectors including such DNA, cells transfected with the vectors, and recombinant methods for providing C2/4GnT. The enzyme C2/4GnT and C2/4GnT-active derivatives thereof are disclosed, in particular soluble derivatives comprising the catalytically active domain of C2/4GnT. Further, the invention discloses methods of obtaining 1,6-N-acetyl glucosaminyl glycosylated saccharides, glycopeptides or glycoproteins by use of an enzymically active C2/4GnT protein or fusion protein thereof or by using cells stably transfected with a vector including DNA encoding an enzymatically active C2/4GnT protein as an expression system for recombinant production of such glycopeptides or glycoproteins. Also a method for the identification for the identification of DNA sequence variations in the C2/4GnT gene by isolating DNA from a patient, amplifying C2/4GnT-coding exons by PCR, and detecting the presence of DNA sequence variation are disclosed.

  一种新基因定义了一种新型人类 UDP-GlcNAc：Gal/Gl cNAc&bgr; 1-3GalNAc a&bgr;1, 6GlcNAc-转移酶，称为 C2/4GnT，具有独特的酶学特性。C2/4GnT 的酶活性与以前发现的该基因家族的酶不同。本发明公开了通过氨基酸缺失、取代或插入的方式编码 C2/4GnT 及其衍生物的分离 DNA 分子和 DNA 构建体，这些 DNA 分子和 DNA 构建体具有 C2/4GnT 的活性，本发明还公开了包括这种 DNA 的克隆和表达载体、用载体转染的细胞以及提供 C2/4GnT 的重组方法。本发明公开了 C2/4GnT 酶及其 C2/4GnT 活性衍生物，特别是包含 C2/4GnT 催化活性结构域的可溶性衍生物。此外，本发明还公开了通过使用具有酶活性的 C2/4GnT 蛋白或其融合蛋白，或通过使用包括编码具有酶活性的 C2/4GnT 蛋白的 DNA 的载体稳定转染的细胞作为重组生产此类糖肽或糖蛋白的表达系统，获得 1,6-N- 乙酰葡糖胺基糖基化糖、糖肽或糖蛋白的方法。此外，还公开了一种用于鉴定 C2/4GnT 基因中 DNA 序列变异的方法，即从患者体内分离 DNA，通过 PCR 扩增 C2/4GnT 编码外显子，并检测是否存在 DNA 序列变异。
• UDP-N-acetylglucosamine: galactose-Beta 1,3-N-A-acetylgalactosamine-alpha-R/N-acetylglucosamine-Beta 1,3-N-acetylgalactosamine-alpha-R (GIcNAc to GalNac) Beta 1,6-N-acetylglucosaminyltransferase, C2/4GnT
  申请人：Clausen Henrik

  公开号：US20050042727A1

  公开（公告）日：2005-02-24

  A novel gene defining a novel human UDP-GlcNAc: Gal/Gl cNAcβ 1-3GalNAc αβ1, 6GlcNAc-transferase, termed C2/4GnT, with unique enzymatic properties is disclosed. The enzymatic activity of C2/4GnT is shown to be distinct from that of previously identified enzymes of this gene family. The invention discloses isolated DNA molecules and DNA constructs encoding C2/4GnT and derivatives thereof by way of amino acid deletion, substitution or insertion exhibiting C2/4GnT activity, as well as cloning and expression vectors including such DNA, cells transfected with the vectors, and recombinant methods for providing C2/4GnT. The enzyme C2/4GnT and C2/4GnT-active derivatives thereof are disclosed, in particular soluble derivatives comprising the catalytically active domain of C2/4GnT. Further, the invention discloses methods of obtaining 1,6-N-acetyl glucosaminyl glycosylated saccharides, glycopeptides or glycoproteins by use of an enzymically active C2/4GnT protein or fusion protein thereof or by using cells stably transfected with a vector including DNA encoding an enzymatically active C2/4GnT protein as an expression system for recombinant production of such glycopeptides or glycoproteins. Also a method for the identification for the identification of DNA sequence variations in the C2/4GnT gene by isolating DNA from a patient, amplifying C2/4GnT-coding exons by PCR, and detecting the presence of DNA sequence variation are disclosed.

  一个新基因定义了一种新的人类 UDP-GlcNAc：Gal/Gl cNAcβ 1-3GalNAc αβ1, 6GlcNAc-转移酶，称为 C2/4GnT，具有独特的酶学特性。C2/4GnT 的酶活性与以前发现的该基因家族的酶不同。本发明公开了通过氨基酸缺失、取代或插入的方式编码 C2/4GnT 及其衍生物的分离 DNA 分子和 DNA 构建体，这些 DNA 分子和 DNA 构建体具有 C2/4GnT 的活性，本发明还公开了包括这种 DNA 的克隆和表达载体、用载体转染的细胞以及提供 C2/4GnT 的重组方法。本发明公开了 C2/4GnT 酶及其 C2/4GnT 活性衍生物，特别是包含 C2/4GnT 催化活性结构域的可溶性衍生物。此外，本发明还公开了通过使用具有酶活性的 C2/4GnT 蛋白或其融合蛋白，或通过使用包括编码具有酶活性的 C2/4GnT 蛋白的 DNA 的载体稳定转染的细胞作为重组生产此类糖肽或糖蛋白的表达系统，获得 1,6-N- 乙酰葡糖胺基糖基化糖、糖肽或糖蛋白的方法。此外，还公开了一种用于鉴定 C2/4GnT 基因中 DNA 序列变异的方法，即从患者体内分离 DNA，通过 PCR 扩增 C2/4GnT 编码外显子，并检测是否存在 DNA 序列变异。
• UDP-N- ACETYL GLUCOSAMINE: GALACTOSE-BETA1, 3-N- ACETYL GALACTOSAMINE-ALPHA-R/ N- ACETYL GLUCOSAMINE -BETA1, 3-N- ACETYL GALACTOSAMINE- ALPHA-R (GlcNAc TO GalNAc) BETA1, 6-N- ACETYL GLUCOSAMINYL TRANSFERASE, C2/4GnT
  申请人：Glycozym ApS

  公开号：EP1135472B1

  公开（公告）日：2006-11-29