2'-脱氧腺苷 5'-O-(1-硫代三磷酸酯) | 64145-28-4

• 分子结构分类: 有机化合物 - 核苷、核苷酸和类似物 - 嘌呤核苷
• 中文名称: 2'-脱氧腺苷 5'-O-(1-硫代三磷酸酯)
• 中文别名: 2'-脱氧腺苷5'-O-(1-硫代三磷酸酯)
• 英文名称: 2'-deoxyadenosine 5'-O-(1-thiotriphosphate)
• 英文别名: dATP(α-S)；dATPαS；dATPalpha S；Datpalphas；[[(2R,3S,5R)-5-(6-aminopurin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphinothioyl] phosphono hydrogen phosphate
• CAS: 64145-28-4
• 化学式: C₁₀H₁₆N₅O₁₁P₃S
• 分子量: 507.251
• InChiKey: CCPIKNHZOWQALM-DLQJRSQOSA-N

物化性质

• 沸点：
  865.6±75.0 °C(Predicted)
• 密度：
  2.45±0.1 g/cm3(Predicted)

计算性质

• 辛醇/水分配系数(LogP)：
  -2.7
• 重原子数：
  30
• 可旋转键数：
  8
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.5
• 拓扑面积：
  274
• 氢给体数：
  6
• 氢受体数：
  16

反应信息

• 作为产物：
  描述：

  3'-O-acetyl-N-benzoyl-2'-deoxyadenosine 在 吡啶 、 氨 、 tris(tetra-n-butylammonium) hydrogen pyrophosphate 、 sulfur 、 1,8-二氮杂双环[5.4.0]十一碳-7-烯 作用下， 以 乙腈 为溶剂， 反应 24.0h， 生成 2'-脱氧腺苷 5'-O-(1-硫代三磷酸酯)
  参考文献：
  名称：

  Synthesis of Nucleoside α-Thiotriphosphates via an Oxathiaphospholane Approach^†

  摘要：

  Nucleoside 5 '-O-(alpha-thiotriphosphates) were obtained in reactions of the appropriate nucleoside 5 '-O-(2-thio-1,3,2-oxathiaphospholanes) with pyrophosphate in the presence of DBU. The presented method allows also for preparation of alpha-seleno congeners and corresponding a-modified diphosphates.

  DOI：

  10.1021/ol050617r

文献信息

• Strand displacement amplification
  申请人：Becton Dickinson and Company

  公开号：EP0497272A1

  公开（公告）日：1992-08-05

  This invention relates a nucleic acid target amplification and detection method which operates at a single temperature and makes use of a polymerase in conjunction with an endonuclease that will nick the polymerized strand such that the polymerase will displace the strand without digestion while generating a newly polymerized strand.

  本发明涉及一种核酸目标扩增和检测方法，该方法在单一温度下操作，利用聚合酶和内切酶，内切酶将聚合链切口，使聚合酶在不消化的情况下置换链，同时产生新的聚合链。
• Strand displacement amplification using thermophilic enzymes
  申请人：Becton Dickinson and Company

  公开号：EP0684315A1

  公开（公告）日：1995-11-29

  Strand Displacement Amplification methods (thermophilic SDA) which can be performed over a broad temperature range (37 ° C to 70 ° C). The preferred temperature range for thermophilic SDA is 50 °C to 70 °C. It has been found that certain thermophilic restriction endonucleases are capable of nicking the hemimodified restriction endonuclease recognition/cleavage site as required by SDA and dissociating from the site. It has further been found that certain thermophilic polymerases are capable of extending from the nick while displacing the downstream strand. Thermophilic SDA, because of reaction temperatures higher than previously possible with conventional SDA enzyme systems, has improved specificity and efficiency, reduced nonspecific background amplification, and potentially improved yields of amplification products. In addition, the need to add the enzymes in a separate step after the initial heat denaturation of double stranded targets is eliminated when enzymes capable of tolerating the denaturation temperature are used.

  链置换扩增方法（嗜热 SDA），可在较宽的温度范围（37 ° C 至 70 ° C）内进行。嗜热 SDA 的首选温度范围为 50 ℃ 至 70 ℃。研究发现，某些嗜热限制性内切酶能够按照 SDA 的要求，剔除半改性限制性内切酶识别/清除位点，并与该位点分离。研究还发现，某些嗜热聚合酶能够从缺口处延伸，同时取代下游链。嗜热 SDA 的反应温度比传统 SDA 酶系统要高，因此提高了特异性和效率，减少了非特异性背景扩增，并有可能提高扩增产物的产量。此外，如果使用能够耐受变性温度的酶，就不需要在双链目标物初始热变性后的单独步骤中添加酶。
• Species-specific detection of Mycobacterium kansasii
  申请人：Becton Dickinson and Company

  公开号：EP0707075A2

  公开（公告）日：1996-04-17

  M. kansasii-specific oligonucleotide amplification primers and methods for detecting and identifying M. kansasii nucleic acids using the amplification primers. One hundred percent of the clinical and environmental M. kanasii isolates tested were positive in amplification assays using the inventive amplification primers, with no cross-reactivity in other species of mycobacteria or closely related non-mycobacteria.

  堪萨斯分枝杆菌特异性寡核苷酸扩增引物和使用扩增引物检测和鉴定堪萨斯分枝杆菌核酸的方法。在使用本发明的扩增引物进行的扩增检测中，100% 的临床和环境堪萨斯分枝杆菌分离物检测结果呈阳性，与其他种类的分枝杆菌或密切相关的非分枝杆菌没有交叉反应。
• Amplification and detection of mycobacteria nucleic acids
  申请人：Becton Dickinson and Company

  公开号：EP0725148A2

  公开（公告）日：1996-08-07

  Methods for identification or detection of a species of an organism or a group of related species of an organism by species non-specific amplification of a target sequence followed by species- or group-specific detection of the amplification products. Also provided are a target sequence which is amplifiable in multiple species of mycobacteria using a single pair of amplification primers and species- and group-specific detector probes for hybridization to the assay regioin of the amplified target. Blocking oligonucleotides are employed to allow discrimination among species in which the amplified target sequences are sufficiently similar that they cross-hybridize to an assay probe.

  通过对目标序列进行物种非特异性扩增，然后对扩增产物进行物种或群体特异性检测，从而鉴定或检测生物的一个物种或生物的一组相关物种的方法。此外，还提供了一种可在多个分枝杆菌物种中扩增的目标序列，使用一对扩增引物和物种及群组特异性检测探针与扩增目标的检测区域杂交。使用阻断寡核苷酸可以在扩增的目标序列足够相似以至于与检测探针交叉杂交的物种之间进行区分。
• Method to access nucleic acids from cells
  申请人：Becton Dickinson and Company

  公开号：EP0795602A2

  公开（公告）日：1997-09-17

  The present invention relates to a sample processing method for rendering cellular components such as nucleic acids in a sample accessible. The method involves subjecting a sample of disrupted cells to sufficient agitation in the presence of particles to separate nucleic acids from other cellular components. A heat lysis process without other lysogenic agents or conditions is the preferred method of disrupting cells for the sample Once accessed, the nucleic acids may be used in various molecular biology procedures.

  本发明涉及一种样品处理方法，用于使样品中的细胞成分（如核酸）变得易于获取。该方法包括在有微粒存在的情况下对被破坏的细胞样本进行充分搅拌，使核酸与其他细胞成分分离。在不使用其它裂解剂或在不使用其它裂解剂或不使用其它裂解条件的情况下进行热裂解是破坏细胞样品的首选方法。