甘氨酸对硝基苯胺 | 1205-88-5

• 物质功能分类: 生物化工 - 氨基酸及其衍生物 - 甘氨酸类衍生物
• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 羧酸及其衍生物
• 中文名称: 甘氨酸对硝基苯胺
• 英文名称: glycine p-nitroanilide
• 英文别名: 2-Amino-N-(4-nitrophenyl)acetamide；glycine p-nitro-anilide；Gly-pNA；glycine-(4-nitro-anilide)；Glycin-(4-nitro-anilid)
• CAS: 1205-88-5
• 化学式: C₈H₉N₃O₃
• 分子量: 195.178
• InChiKey: VZZMNSJSZXVCCW-UHFFFAOYSA-N

物化性质

• 熔点：
  157 °C
• 沸点：
  451.7±30.0 °C(Predicted)
• 密度：
  1.413±0.06 g/cm3(Predicted)

计算性质

• 辛醇/水分配系数(LogP)：
  0.8
• 重原子数：
  14
• 可旋转键数：
  2
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.12
• 拓扑面积：
  101
• 氢给体数：
  2
• 氢受体数：
  4

安全信息

• WGK Germany：
  3
• 海关编码：
  2924299090
• 储存条件：
  -20°C

SDS

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Section 1. IDENTIFICATION OF THE SUBSTANCE/MIXTURE
Product identifiers
Product name : Glycine p-nitroanilide
CAS-No. : 1205-88-5
Relevant identified uses of the substance or mixture and uses advised against
Identified uses : Laboratory chemicals, Manufacture of substances

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Section 2. HAZARDS IDENTIFICATION
Classification of the substance or mixture
Not a hazardous substance or mixture according to Regulation (EC) No. 1272/2008.
This substance is not classified as dangerous according to Directive 67/548/EEC.
Label elements
The product does not need to be labelled in accordance with EC directives or respective national laws.
Other hazards - none

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Section 3. COMPOSITION/INFORMATION ON INGREDIENTS
Substances
Formula : C8H9N3O3
Molecular Weight : 195,18 g/mol

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Section 4. FIRST AID MEASURES
Description of first aid measures
If inhaled
If breathed in, move person into fresh air. If not breathing, give artificial respiration.
In case of skin contact
Wash off with soap and plenty of water.
In case of eye contact
Flush eyes with water as a precaution.
If swallowed
Never give anything by mouth to an unconscious person. Rinse mouth with water.
Most important symptoms and effects, both acute and delayed
To the best of our knowledge, the chemical, physical, and toxicological properties have not been
thoroughly investigated.
Indication of any immediate medical attention and special treatment needed
no data available

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Section 5. FIREFIGHTING MEASURES
Extinguishing media
Suitable extinguishing media
Use water spray, alcohol-resistant foam, dry chemical or carbon dioxide.
Special hazards arising from the substance or mixture
Carbon oxides, nitrogen oxides (NOx)
Advice for firefighters
Wear self contained breathing apparatus for fire fighting if necessary.
Further information
no data available

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Section 6. ACCIDENTAL RELEASE MEASURES
Personal precautions, protective equipment and emergency procedures
Avoid dust formation. Avoid breathing vapors, mist or gas.
Environmental precautions
Do not let product enter drains.
Methods and materials for containment and cleaning up
Sweep up and shovel. Keep in suitable, closed containers for disposal.
Reference to other sections
For disposal see section 13.

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Section 7. HANDLING AND STORAGE
Precautions for safe handling
Provide appropriate exhaust ventilation at places where dust is formed.Normal measures for preventive fire
protection.
Conditions for safe storage, including any incompatibilities
Store in cool place. Keep container tightly closed in a dry and well-ventilated place.
Recommended storage temperature: -20 °C
Specific end uses
no data available

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Section 8. EXPOSURE CONTROLS/PERSONAL PROTECTION
Control parameters
Components with workplace control parameters
Exposure controls
Appropriate engineering controls
General industrial hygiene practice.
Personal protective equipment
Eye/face protection
Use equipment for eye protection tested and approved under appropriate government standards
such as NIOSH (US) or EN 166(EU).
Skin protection
Handle with gloves. Gloves must be inspected prior to use. Use proper glove removal technique
(without touching glove's outer surface) to avoid skin contact with this product. Dispose of
contaminated gloves after use in accordance with applicable laws and good laboratory practices.
Wash and dry hands.
The selected protective gloves have to satisfy the specifications of EU Directive 89/686/EEC and
the standard EN 374 derived from it.
Body Protection
Choose body protection in relation to its type, to the concentration and amount of dangerous
substances, and to the specific work-place., The type of protective equipment must be selected
according to the concentration and amount of the dangerous substance at the specific workplace.
Respiratory protection
Respiratory protection is not required. Where protection from nuisance levels of dusts are desired,
use type N95 (US) or type P1 (EN 143) dust masks. Use respirators and components tested and
approved under appropriate government standards such as NIOSH (US) or CEN (EU).

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Section 9. PHYSICAL AND CHEMICAL PROPERTIES
Information on basic physical and chemical properties
a) Appearance Form: solid
b) Odour no data available
c) Odour Threshold no data available
d) pH no data available
e) Melting point/freezing no data available
point
f) Initial boiling point and no data available
boiling range
g) Flash point no data available
h) Evaporation rate no data available
i) Flammability (solid, gas) no data available
j) Upper/lower no data available
flammability or
explosive limits
k) Vapour pressure no data available
l) Vapour density no data available
m) Relative density no data available
n) Water solubility no data available
o) Partition coefficient: n- no data available
octanol/water
p) Autoignition no data available
temperature
q) Decomposition no data available
temperature
r) Viscosity no data available
s) Explosive properties no data available
t) Oxidizing properties no data available
Other safety information
no data available

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Section 10. STABILITY AND REACTIVITY
Reactivity
no data available
Chemical stability
no data available
Possibility of hazardous reactions
no data available
Conditions to avoid
no data available
Incompatible materials
no data available
Hazardous decomposition products
Other decomposition products - no data available

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Section 11. TOXICOLOGICAL INFORMATION
Information on toxicological effects
Acute toxicity
no data available
Skin corrosion/irritation
no data available
Serious eye damage/eye irritation
no data available
Respiratory or skin sensitization
no data available
Germ cell mutagenicity
no data available
Carcinogenicity
IARC: No component of this product present at levels greater than or equal to 0.1% is identified as
probable, possible or confirmed human carcinogen by IARC.
Reproductive toxicity
no data available
Specific target organ toxicity - single exposure
no data available
Specific target organ toxicity - repeated exposure
no data available
Aspiration hazard
no data available
Potential health effects
Inhalation
May be harmful if inhaled. May cause respiratory tract irritation.
Ingestion May be harmful if swallowed.
Skin May be harmful if absorbed through skin. May cause skin irritation.
Eyes May cause eye irritation.
Signs and Symptoms of Exposure
To the best of our knowledge, the chemical, physical, and toxicological properties have not been
thoroughly investigated.
Additional Information
RTECS: Not available

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Section 12. ECOLOGICAL INFORMATION
Toxicity
no data available
Persistence and degradability
no data available
Bioaccumulative potential
no data available
Mobility in soil
no data available
Results of PBT and vPvB assessment
no data available
Other adverse effects
no data available

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Section 13. DISPOSAL CONSIDERATIONS
Waste treatment methods
Product
Offer surplus and non-recyclable solutions to a licensed disposal company.
Contaminated packaging
Dispose of as unused product.

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Section 14. TRANSPORT INFORMATION
UN number
ADR/RID: - IMDG: - IATA: -
UN proper shipping name
ADR/RID: Not dangerous goods
IMDG: Not dangerous goods
IATA: Not dangerous goods
Transport hazard class(es)
ADR/RID: - IMDG: - IATA: -
Packaging group
ADR/RID: - IMDG: - IATA: -
Environmental hazards
ADR/RID: no IMDG Marine pollutant: no IATA: no
Special precautions for user
no data available

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Section 15. REGULATORY INFORMATION
This safety datasheet complies with the requirements of Regulation (EC) No. 1907/2006.
Safety, health and environmental regulations/legislation specific for the substance or mixture
no data available
Chemical Safety Assessment
no data available

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Section 16. OTHER INFORMATION
Further information
Copyright 2012 Co. LLC. License granted to make unlimited paper copies for internal use
only.
The above information is believed to be correct but does not purport to be all inclusive and shall be
used only as a guide. The information in this document is based on the present state of our knowledge
and is applicable to the product with regard to appropriate safety precautions. It does not represent any
guarantee of the properties of the product. Corporation and its Affiliates shall not be held
liable for any damage resulting from handling or from contact with the above product. See
and/or the reverse side of invoice or packing slip for additional terms and conditions of sale.

反应信息

• 作为反应物：
  描述：

  甘氨酸对硝基苯胺 在 氢溴酸 、 N,N'-二环己基碳二亚胺 作用下， 以 二氯甲烷 、 溶剂黄146 、 N,N-二甲基甲酰胺 为溶剂， 反应 17.5h， 生成 acetylleucyl-leucyl-glycine p-nitroanilide
  参考文献：
  名称：

  Kasafirek, Evzen; Fukal, Ladislav; Kas, Jan, Collection of Czechoslovak Chemical Communications, 1988, vol. 53, # 12, p. 3197 - 3205

  摘要：

  DOI：
• 作为产物：
  描述：

  Nα-benzyloxycarbonyl-L-glycyl-p-nitroanilide 在 氢溴酸 作用下， 以 溶剂黄146 为溶剂， 生成 甘氨酸对硝基苯胺
  参考文献：
  名称：

  Synthesis of chromogenic substrates specific for human spleen fibrinolytic proteinase (SFP) and human leukocyte elastase (LE).

  摘要：

  通过常规方法合成了多种肽苯胺，目的是获得人类脾纤维蛋白溶解酶（SFP）和人类白细胞弹性蛋白酶（LE）的特定底物，以便比较SFP和LE的底物特异性。研究发现，琥珀酰三肽对硝基苯胺（Suc-Tyr-Leu-X-pNA）中的P1 Val化合物在SFP和LE的水解反应中表现出最高的kcat/Km值，然而，四肽Suc-Ala-Tyr-Leu-Val-pNA（SFP和LE的水解反应的kcat/Km值（M-1S-1）：分别为84000和48000）是SFP和LE的首选显色底物，因为它具有在缓冲液中的高溶解性和适中的kcat/Km值。发现SFP的底物特异性与LE的相似。

  DOI：

  10.1248/cpb.30.4060

文献信息

• Studies on the β-Turn of Peptides. V. Turn Preference of Peptide Sequence Analyzed by Conformational Energy Calculation on Dipeptide Models and by CD Spectra of Chromophoric Derivatives
  作者：Masao Kawai、Kazuki Sato、Rika Sugawara、Ukon Nagai

  DOI：10.1246/bcsj.56.3005

  日期：1983.10

  Empirical conformational energy calculations were performed on Ac–L-Pro–D-Ala–NHCH3 and Ac–D-Ala–L-Pro–NHCH3. In order to study the turn tendency of the dipeptide sequences turn probabilities and average interterminal distances were calculated. Much larger turn preference of D-Ala containing sequences was indicated compared with the corresponding L-Ala containing sequences. The turn tendency of the

  对 Ac-L-Pro-D-Ala-NHCH3 和 Ac-D-Ala-L-Pro-NHCH3 进行了经验构象能计算。为了研究二肽序列的转向趋势，计算转向概率和平均末端间距离。与相应的含有 L-Ala 的序列相比，表明含有 D-Ala 的序列有更大的转向偏好。二肽序列的转向趋势显示为：L-Pro-D-Ala>D-Ala-L-Pro>L-Pro-L-Ala>L-Ala-L-Pro。该顺序与在相应二肽序列的 N-和 C-末端分别具有 2,4-二硝基苯基甘氨酰和甘氨酸-对硝基苯胺的发色衍生物的 CD 光谱中棉花效应的大小顺序一致.
• Purification of an Aminopeptidase Preferentially Releasing N-terminal Alanine from Cucumber Leaves and Its Identification as a Plant Aminopeptidase N
  作者：Yasuo YAMAUCHI、Yukinori EJIRI、Kiyoshi TANAKA

  DOI：10.1271/bbb.65.2802

  日期：2001.1

  In this study, a highly active foliar aminopeptidase preferentially releasing N-terminal alanine from artificial substrates was purified and characterized from cucumber (Cucumis sativus L. suyo). The enzyme had a molecular mass of 200 kDa consisting of two subunits of 95 kDa. It was a metalloprotease the pH optimum of which was 8 to 9. It cleaved Ala-, Gly-, Met-, Ser-, Leu-, Lys-, and Arg artificial substrates. An internal amino acid sequence was similar to those of aminopeptidase N (clan MA, family M1) of microorganisms, and was very similar to that of a putative aminopeptidase N of Arabidopsis thaliana. From these results, the highly active aminopeptidase in cucumber leaves was identified to be a plant aminopepitdase N.

  在本研究中，从黄瓜（Cucumis sativus L. suyo）中纯化和鉴定了一种高活性的叶面氨基肽酶，该酶优先从人工底物中释放N端丙氨酸。该酶的分子质量为200 kDa，由两个95 kDa的亚基组成。它是一种金属蛋白酶，其最适pH为8至9。它能切割Ala-、Gly-、Met-、Ser-、Leu-、Lys-和Arg的人工底物。其内部氨基酸序列与微生物的氨基肽酶N（MA家族，M1家族）相似，并且与拟南芥中假定的氨基肽酶N非常相似。根据这些结果，黄瓜叶中高活性的氨基肽酶被鉴定为植物氨基肽酶N。
• Purification and Some Properties of an Aminopeptidase from the Seeds of<i>Cannabis sativa</i>
  作者：Kazunari ARIMA、Tetsuya UCHIKOBA、Masayuki SHIMADA、Hiroo YONEZAWA、Makoto KANEDA

  DOI：10.1271/bbb.64.1055

  日期：2000.1

  (HSA) with a molecular mass of 78 kDa was purified from hemp (Cannabis sativa) seeds. The activity was inhibited by monoiodeacetic acid, p-chloromercuri-phenylsulfonic acid, and Zn2+ ion. The specificity of HSA was similar to that of a leucyl aminopeptidase [EC 3.4.11.1] from mammalian cytosol. However, other enzyme properties were different from these of leucyl aminopeptidase.

  从大麻（大麻）种子中纯化出分子量为78 kDa的氨基肽酶（HSA）。活性被单碘乙酸，对氯汞基苯磺酸和Zn2 +离子抑制。HSA的特异性类似于来自哺乳动物细胞质的亮氨酰肽酶[EC 3.4.11.1]。但是，其他酶的性质不同于亮氨酰氨肽酶。
• Substrate Specificity of Aminopeptidase from the Mid-gut Gland of the Scallop (<i>Patinopecten yessoensis</i>)
  作者：Hironori UMETSU、Mito ARAI、Toshinori OTA、Kaoru ABE、Hidemitsu UCHIZAWA、Kazuo SASAKI

  DOI：10.1271/bbb.68.945

  日期：2004.1

  An action for various peptides and a kinetic study for amino acid p-nitroanilides (pNAs) and 4-methylcoumaryl-7-amides (MCAs) were performed with purified aminopeptidase from the mid-gut of the scallop. The enzyme preferred dipeptides having Ala, Met, and Phe in the amino-terminal or the penultimate position from the amino-termini. The catalytic efficiencies, kcat⁄Km values for Ala-pNA and MCA were the highest in the tested substrates, and those for pNA and MCA substrates having Met or Phe were the next highest. The enzyme was found to be a new alanine-specific aminopeptidase.

  利用从扇贝中肠中纯化的氨肽酶进行了一系列肽类底物的作用和氨基酸p-硝基苯胺（pNA）及4-甲基香豆素-7-酰胺（MCA）的动力学研究。该酶偏好具有Ala、Met和Phe在氨基端的N-末端或倒数第二位的二肽底物。在测试的底物中，Ala-pNA和MCA的催化效率（kcat/Km值）最高，而具有Met或Fhe的pNA和MCA底物其次。研究发现该酶是一种新型的特异性亮氨酸氨肽酶。
• Polarographic study of hydrolysis of [8-lysine]vasopressin and its derivatives with blood serum of pregnant women
  作者：Mikuláš Chavko、Michal Bartík、Evžen Kasafírek

  DOI：10.1135/cccc19801099

  日期：——

  A polarographic study of the hydrolysis of [8-lysine]vasopressin and some hormonogens of the vasopressin series with the blood serum of women in the last week of pregnancy was studied. The dependence of hydrolysis on pH (pH optimum: 7.4-7.50, substrate concentration (K_m 1.2 . 10^-5M), pH stability and thermal stability were determined. The rate of hydrolysis of individual vasopressin analogues decreases in the order: [8-lysine]vasopressin > N^α-glycyl-prolyl[8-lysine]-vasopressin > N^α-leucyl-[8-lysine]vasopressin > N^α-alanyl-[8-lysine]vasopressin > N^α-phenyl alanyl-[8-lysine]vasopressin > N^α-diglycyl-[8-lysine]vasopressin > N^α-prolyl-[8-lysine]vasopressin > Nα-triglycyl-[8-lysine]vasopressin > Nα-sarcosyl-glycyl-[8-lysine]vasopressin. The degree of hydrolysis gradually increases to a multiple with the length of the pregnancy in consequence of the presence of oxytocine. However, vasopressin is also hydrolysed to a small extent with the enzymes from the blood sera of non-pregnant women. Under similar analytical conditions oxytocin was not hydrolysed with the sera of non-pregnant women and therefore oxytocin is a more suitable substrate than vasopressin for polarographic determination of serum oxytocinase.

  对孕妇血清中[8-赖氨酸]加压素及一些加压素类激素的水解进行了极谱研究。研究了水解对pH的依赖性（pH最适值：7.4-7.50，底物浓度（Km 1.2 . 10^-5M），pH稳定性和热稳定性。各个加压素类似物的水解速率按顺序递减：[8-赖氨酸]加压素 > Nα-甘氨酰-脯氨酰[8-赖氨酸]-加压素 > Nα-亮氨酰-[8-赖氨酸]加压素 > Nα-丙氨酰-[8-赖氨酸]加压素 > Nα-苯基丙氨酰-[8-赖氨酸]加压素 > Nα-二甘氨酰-[8-赖氨酸]加压素 > Nα-脯氨酰-[8-赖氨酸]加压素 > Nα-三甘氨酰-[8-赖氨酸]加压素 > Nα-肌氨酰基甘氨酰-[8-赖氨酸]加压素。水解程度随着妊娠时间的延长逐渐增加，因为存在催产素。然而，加压素也在非孕妇血清中的酶的作用下被水解，但程度较小。在类似的分析条件下，催产素在非孕妇血清中不被水解，因此催产素比加压素更适合用于极谱测定血清催产素酶。