3-硝基苯-Alpha-D-吡喃半乳糖苷 | 3150-25-2

• 物质功能分类: 生物化工 - 糖类化合物 - 单糖
• 分子结构分类: 有机化合物 - 有机氧化合物
• 中文名称: 3-硝基苯-Alpha-D-吡喃半乳糖苷
• 中文别名: 3-硝基苯基-Α-D-吡喃半乳糖苷；3-硝基苯基-alpha-D-吡喃葡糖；3-硝基苯-α-D-吡喃半乳糖苷
• 英文名称: meta-nitrophenyl-α-D-galactopyranoside
• 英文别名: meta-nitrophenyl α-galactopyranoside；m-nitrophenyl-α-D-galactopyranoside；3-nitrophenyl α-D-galactoside；MNPG；m-Nitrophenyl-α-D-galactopyranosid；m-Nitrophenyl-α-D-galactosid；3-Nitrophenyl alpha-D-galactopyranoside；(2R,3R,4S,5R,6R)-2-(hydroxymethyl)-6-(3-nitrophenoxy)oxane-3,4,5-triol
• CAS: 3150-25-2；20838-44-2；28541-80-2；56140-66-0；72200-66-9；52571-71-8
• 化学式: C₁₂H₁₅NO₈
• 分子量: 301.253
• InChiKey: VCCMGHVCRFMITI-IIRVCBMXSA-N

物化性质

• 密度：
  1.599±0.06 g/cm3 (20 ºC 760 Torr)

计算性质

• 辛醇/水分配系数(LogP)：
  -0.5
• 重原子数：
  21
• 可旋转键数：
  3
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.5
• 拓扑面积：
  145
• 氢给体数：
  4
• 氢受体数：
  8

安全信息

• 海关编码：
  2932999099

反应信息

• 作为产物：
  描述：

  1,2,3,4,6-D-葡萄糖五乙酸酯 在 三氟甲磺酸 、 苄胺 、 sodium hydroxide 作用下， 以 甲醇 、 N,N-二甲基甲酰胺 为溶剂， 生成 3-硝基苯-Alpha-D-吡喃半乳糖苷
  参考文献：
  名称：

  负担得起的，基于聚合物的多价抑制剂对霍乱毒素B亚基的强抑制作用。

  摘要：

  霍乱是一种潜在的致命细菌感染，影响发展中国家的许多人。它是由霍乱弧菌分泌的AB5毒素霍乱毒素（CT）引起的。该毒素包含与肠道细胞表面结合的有毒的A亚基和五聚体B亚基。已经合成了几种毒素的单价和多价抑制剂，但是对于发展中国家的实际应用而言太复杂和昂贵。间硝基苯基α-半乳糖苷（MNPG）是已知的CT有希望的配体，在此合成了基于MNPG的单价和多价化合物。我们提出了MNPG的合成，产率大大提高，并且与多价支架连接时的使用。我们使用经济的聚合物作为多价支架，即聚丙烯酰胺，葡聚糖和超支化聚甘油（hPGs）。铜催化的炔烃叠氮化物环加成反应（CuAAC）产生了抑制剂，这些抑制剂已通过ELISA型测定法和肠类器官溶胀抑制测定法进行了测试。抑制性能的变化取决于聚合物的类型，最有效的偶联物在纳摩尔范围内显示出IC50值。

  DOI：

  10.1021/acs.bioconjchem.8b00902

文献信息

• Methods and compositions for enzyme complementation assays using the omega region of beta-galactosidase
  申请人：MICROGENICS CORPORATION

  公开号：EP0514173A2

  公开（公告）日：1992-11-19

  The use of omega-acceptor and omega-donor polypeptides (comprising about two-thirds and one-third of the β-galactosidase molecule amino and carboxyl termini, respectively), prepared by recombinant DNA techniques, DNA synthesis, or chemical polypeptide synthesis techniques, which are capable of interacting to form an active enzyme complex having catalytic activity characteristic of β-galactosidase, is described along with improved methods and novel compositions for enzyme complementation assays for qualitative and quantitative determination of a suspected analyte in a sample.

  通过重组 DNA 技术、DNA 合成技术或化学多肽合成技术制备的欧米伽受体多肽和欧米伽供体多肽（分别占 β-半乳糖苷酶分子氨基端和羧基端约三分之二和三分之一）、本研究描述了能够相互作用形成具有 β-半乳糖苷酶催化活性特征的活性酶复合物的方法，以及用于定性和定量测定样品中可疑分析物的酶互补测定的改进方法和新型组合物。
• Detection of complementary nucleotide sequences
  申请人：MICROGENICS CORPORATION

  公开号：EP0530998A1

  公开（公告）日：1993-03-10

  The invention relates to a method for detection of a specific nucleic acid sequence which comprises    forming a reaction mixture by combining (1) a sample suspected of containing a nucleic acid; (2) a probe/enzyme donor polypeptide conjugate comprising (a) an enzyme donor polypeptide sequence comprising a β-galactosidase fragment; and (b) a single-stranded oligonucleotide sequence attached to (a) and capable of hybridizing with said nucleic acid; (3) an enzyme acceptor polypeptide capable of forming an active enzyme upon complementation with said enzyme donor fragment; and (4) a substrate for β-galactosidase; and    detecting hybridization of said probe/enzyme donor conjugate to said sample nucleic acid to form a double strand-specific sequence by determining the amount or rate of enzyme activity on said substrate in said reaction mixture. The method can also include a "proof reading" function by incubating the hybridized probe with at least one double-strand-specific, sequence-specific restriction endonuclease. Novel kits for use in carrying out the method are also included.

  本发明涉及一种检测特定核酸序列的方法，该方法包括 将以下物质混合形成反应混合物 (1) 怀疑含有核酸的样品； (2) 探针/酶供体多肽共轭物，包括 (a) 由 β-半乳糖苷酶片段组成的酶供体多肽序列；和 (b) 连接到(a)上并能与所述核酸杂交的单链寡核苷酸序列； (3) 与所述酶供体片段互补后能形成活性酶的酶受体多肽；以及 (4) β-半乳糖苷酶的底物；以及 通过确定所述反应混合物中所述底物上酶活性的量或速率，检测所述探针/酶供体共轭物与所述样本核酸杂交以形成双链特异性序列。该方法还可以通过将杂交探针与至少一种双链特异性、序列特异性限制性内切酶孵育，实现 "校读 "功能。 还包括用于实施该方法的新型试剂盒。
• Enzyme donor and enzyme-acceptor polypeptides and recombinant DNA vector comprising a DNA coding for said polypeptides
  申请人：MICROGENICS CORPORATION

  公开号：EP0551842A2

  公开（公告）日：1993-07-21

  "Improved methods and novel compositions for enzyme complementation assays for qualitative and quantitative determination of a suspected analyte in a sample. The use of enzyme-acceptor and enzyme-donor polypeptides prepared by recombinant DNA techniques, DNA synthesis or chemical polypeptide synthesis techniques which are capable of interacting to form an active enzyme complex having catalytic activity characteristics of ß-galactosidase is described. Both homogeneous and heterogeneous assays utilizing these polypeptides are described.

  "改进的酶互补测定方法和新型组合物，用于定性和定量测定样品中的可疑分析物。描述了通过重组 DNA 技术、DNA 合成或化学多肽合成技术制备的酶受体和酶供体多肽的使用方法，这些多肽能够相互作用形成具有ß-半乳糖苷酶催化活性特征的活性酶复合物。文中还描述了利用这些多肽进行的均相和异相检测。
• Detection of protein translocation by beta-galactosidase reporter fragment complementation
  申请人：The Board Of Trustees Of The University Of the Leland Stanford Junior University

  公开号：EP2400030A1

  公开（公告）日：2011-12-28

  Methods and compositions are provided for detecting molecular translocations, particularly protein translocations within and between sub-cellular compartments, using at least two components that exhibit a localisation-dependent difference in complementation activity. In particular, alpha-complementing β-galactosidase fragments are provided. These β-galactosidase reporter fragments display significantly enhanced enzymatic activity when one fragment is localised in a membrane. Methods for carrying out no-wash ELISA assays based on the reporter component system are also provided.

  本研究提供了检测分子易位的方法和组合物，特别是利用至少两种在互补活性上表现出定位依赖性差异的成分检测亚细胞区内和亚细胞区之间的蛋白质易位。特别是提供了α-互补β-半乳糖苷酶片段。当一个片段定位在膜中时，这些 β-半乳糖苷酶报告片段显示出明显增强的酶活性。此外，还提供了基于报告片段系统的免清洗 ELISA 检测方法。
• Method for detecting HIV-1 co-receptor tropism
  申请人：Case Western Reserve University

  公开号：US10288613B2

  公开（公告）日：2019-05-14

  A method for determining HIV-1 co-receptor tropism in an HIV-infected patient includes preparing an HIV-1 envelope protein coding sequence from a sample, introducing the HIV-1 envelope protein coding sequence into a first expression construct by providing a plasmid expression vector including a near-full length HIV-1 genome having a yeast uracil biosynthesis gene in place of a HIV-1 env coding sequencing and replacing the yeast uracil biosynthesis gene with the HIV-1 envelope protein coding sequence prepared from the patient sample, and using the first expression construct in a cell to cell fusion assay to determine HIV-1 co-receptor tropism.

  一种用于确定 HIV 感染者的 HIV-1 共受体趋向性的方法包括从样本中制备 HIV-1 包膜蛋白编码序列、通过提供质粒表达载体，将 HIV-1 包膜蛋白编码序列导入第一表达构建体，该质粒表达载体包括接近全长的 HIV-1 基因组，该基因组中的酵母尿嘧啶生物合成基因取代了 HIV-1 env 编码序列，并用从患者样本中制备的 HIV-1 包膜蛋白编码序列取代了酵母尿嘧啶生物合成基因，以及在细胞间融合试验中使用第一表达构建体来确定 HIV-1 共受体趋向性。