(1S,3R,4R,6S,7S)-1-(tert-butyldiphenylsilyloxymethyl)-6-methyl-7-(2-naphthylmethyloxy)-3-(thymin-1-yl)-2,5-dioxabicyclo[2.2.1]heptane | 1197032-94-2

• 分子结构分类: 有机化合物 - 有机氧化合物
• 英文名称: (1S,3R,4R,6S,7S)-1-(tert-butyldiphenylsilyloxymethyl)-6-methyl-7-(2-naphthylmethyloxy)-3-(thymin-1-yl)-2,5-dioxabicyclo[2.2.1]heptane
• 英文别名: 1-[(1S,3R,4R,6S,7S)-1-[[tert-butyl(diphenyl)silyl]oxymethyl]-6-methyl-7-(naphthalen-2-ylmethoxy)-2,5-dioxabicyclo[2.2.1]heptan-3-yl]-5-methylpyrimidine-2,4-dione
• CAS: 1197032-94-2
• 化学式: C₃₉H₄₂N₂O₆Si
• 分子量: 662.858
• InChiKey: SQIYAIFPXNVCNQ-WOLKVUCKSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  5.22
• 重原子数：
  48
• 可旋转键数：
  10
• 环数：
  7.0
• sp3杂化的碳原子比例：
  0.33
• 拓扑面积：
  86.3
• 氢给体数：
  1
• 氢受体数：
  6

反应信息

• 作为反应物：
  描述：

  (1S,3R,4R,6S,7S)-1-(tert-butyldiphenylsilyloxymethyl)-6-methyl-7-(2-naphthylmethyloxy)-3-(thymin-1-yl)-2,5-dioxabicyclo[2.2.1]heptane 在 水 、 triethylamine tris(hydrogen fluoride) 、 三乙胺 、 2,3-二氯-5,6-二氰基-1,4-苯醌 作用下， 以 四氢呋喃 、 二氯甲烷 为溶剂， 反应 16.0h， 生成 (1R,3R,4R,6S,7S)-7-hydroxy-1-hydroxymethyl-6-methyl-3-(thymin-1-yl)-2,5-dioxabicyclo[2.2.1]heptane
  参考文献：
  名称：

  [EN] 5'-SUBSTITUTED BICYCLIC NUCLEOSIDES AND OLIGOMERIC COMPOUNDS PREPARED THEREFROM
  [FR] NUCLÉOSIDES BICYCLIQUES 5'-SUBSTITUÉS ET COMPOSÉS OLIGOMÈRES SYNTHÉTISÉS À PARTIR DESDITS NUCLÉOSIDES

  摘要：

  本文提供了新颖的5'-(S)-CH3取代的双环核苷，以及由其制备的寡聚化合物和使用这些寡聚化合物的方法。更具体地，每个新颖的5'-(S)-CH3取代的双环核苷的呋喃糖环包括一个2'到4'的桥联基团。预计这些5'-(S)-CH3取代的双环核苷将有助于增强它们所并入的寡聚化合物的一个或多个性质，例如增加结合亲和力。在某些实施例中，本文提供的寡聚化合物与靶RNA的部分杂交，导致靶RNA的正常功能丧失。

  公开号：

  WO2011115818A1
• 作为产物：
  描述：

  (3aR,5S,6R,6aR)-2,2-dimethyl-6-(naphthalen-2-ylmethoxy)tetrahydrofuro[3,2-d][1,3]dioxole-5-carbaldehyde 在 甲醇 、 4-二甲氨基吡啶 、 锂硼氢 、 N,O-双三甲硅基乙酰胺 、 cerium(III) chloride 、 草酰氯 、 硫酸 、 溶剂黄146 、 二甲基亚砜 、 三乙胺 、 sodium hydroxide 作用下， 以 四氢呋喃 、 二氯甲烷 、 水 、 乙腈 为溶剂， 反应 123.83h， 生成 (1S,3R,4R,6S,7S)-1-(tert-butyldiphenylsilyloxymethyl)-6-methyl-7-(2-naphthylmethyloxy)-3-(thymin-1-yl)-2,5-dioxabicyclo[2.2.1]heptane
  参考文献：
  名称：

  [EN] 5'-SUBSTITUTED BICYCLIC NUCLEOSIDES AND OLIGOMERIC COMPOUNDS PREPARED THEREFROM
  [FR] NUCLÉOSIDES BICYCLIQUES 5'-SUBSTITUÉS ET COMPOSÉS OLIGOMÈRES SYNTHÉTISÉS À PARTIR DESDITS NUCLÉOSIDES

  摘要：

  本文提供了新颖的5'-(S)-CH3取代的双环核苷，以及由其制备的寡聚化合物和使用这些寡聚化合物的方法。更具体地，每个新颖的5'-(S)-CH3取代的双环核苷的呋喃糖环包括一个2'到4'的桥联基团。预计这些5'-(S)-CH3取代的双环核苷将有助于增强它们所并入的寡聚化合物的一个或多个性质，例如增加结合亲和力。在某些实施例中，本文提供的寡聚化合物与靶RNA的部分杂交，导致靶RNA的正常功能丧失。

  公开号：

  WO2011115818A1

文献信息

• Synthesis and Biophysical Evaluation of 2′,4′-Constrained 2′<i>O</i>-Methoxyethyl and 2′,4′-Constrained 2′<i>O</i>-Ethyl Nucleic Acid Analogues
  作者：Punit P. Seth、Guillermo Vasquez、Charles A. Allerson、Andres Berdeja、Hans Gaus、Garth A. Kinberger、Thazha P. Prakash、Michael T. Migawa、Balkrishen Bhat、Eric E. Swayze

  DOI：10.1021/jo902560f

  日期：2010.3.5

  We have recently shown that combining the structural elements of 2'O-methoxyethyl (MOE) and locked nuclecic acid (LNA) nucleosides yielded a series of nucleoside modification (cMOE, 2',4'-constrained MOE; cEt, 2',4'-constrained ethyl) that display improved potency over MOE and an improved therapeutic index relative to that of LNA antisense oligonucleotides. In this report we present details regarding the synthesis of the cMOE and cEt nucleoside phosphoramidites and the biophysical evaluation of oligonucleotides containing these nucleoside modification. The synthesis of the cMOE and eEt nucleoside phosphoramidites was efficiently accomplished starting from inexpensive commercially available diacetone allofuranose. The synthesis features the use of a seldom used 2-naphthylmethyl protecting group that provides crystalline intermediates during the synthesis and can be cleanly deprotected under mild conditions. The synthesis was greatly facilitated by the crystallinity of a key mono-TBDPS-protected diol intermediate. In the case of the cEt nucleosides, the introduction of the methyl group in either configuration was accomplished in a stereoselective manner. Ring closure of the 2'-hydroxyl group onto a secondary mesylate leaving group with clean inversion of stereochemistry was achieved under suprisingly mild conditions. For the S-cEt modification, the synthesis of all four (thymine, 5-methylcytosine, adenine, and guanine) nucleobase-modified phosphoramidites was accomplished on a multigram scale. Biophysical evaluation of the cMOE- and cEt-containing oligonucletides revealed that they possess hybridization and mismatch discrimination attributes similar to those of LNA but greatly improved resistance to exonuclease digestion.
• [EN] 5'-SUBSTITUTED BICYCLIC NUCLEOSIDES AND OLIGOMERIC COMPOUNDS PREPARED THEREFROM<br/>[FR] NUCLÉOSIDES BICYCLIQUES 5'-SUBSTITUÉS ET COMPOSÉS OLIGOMÈRES SYNTHÉTISÉS À PARTIR DESDITS NUCLÉOSIDES
  申请人：ISIS PHARMACEUTICALS INC

  公开号：WO2011115818A1

  公开（公告）日：2011-09-22

  Provided herein are novel 5'-(S)-CH3 substituted bicyclic nucleosides, oligomeric compounds prepared therefrom and methods of using the oligomeric compounds. More particularly, the furanose ring of each of the novel 5'-(S)-CH3 substituted bicyclic nucleosides includes a 2' to 4' bridging group. The 5'-(S)-CH3 substituted bicyclic nucleosides are expected to be useful for enhancing one or more properties of the oligomeric compounds they are incorporated into such as for example increasing the binding affinity. In certain embodiments, the oligomeric compounds provided herein hybridize to a portion of a target RNA resulting in loss of normal function of the target RNA.

  本文提供了新颖的5'-(S)-CH3取代的双环核苷，以及由其制备的寡聚化合物和使用这些寡聚化合物的方法。更具体地，每个新颖的5'-(S)-CH3取代的双环核苷的呋喃糖环包括一个2'到4'的桥联基团。预计这些5'-(S)-CH3取代的双环核苷将有助于增强它们所并入的寡聚化合物的一个或多个性质，例如增加结合亲和力。在某些实施例中，本文提供的寡聚化合物与靶RNA的部分杂交，导致靶RNA的正常功能丧失。