菜豆双氢异黄酮 | 40105-60-0

• 分子结构分类: 有机化合物 - 苯丙烷和聚酮 - 异黄酮类化合物
• 中文名称: 菜豆双氢异黄酮
• 英文名称: Kievitone
• 英文别名: 3-(2,4-dihydroxyphenyl)-5,7-dihydroxy-8-(3-methylbut-2-enyl)-2,3-dihydrochromen-4-one
• CAS: 40105-60-0
• 化学式: C₂₀H₂₀O₆
• 分子量: 356.4
• InChiKey: MERHMOCEIBOOMA-UHFFFAOYSA-N

物化性质

• 沸点：
  639.6±55.0 °C(Predicted)
• 密度：
  1.384±0.06 g/cm3(Predicted)
• LogP：
  4.600 (est)

计算性质

• 辛醇/水分配系数(LogP)：
  4.1
• 重原子数：
  26
• 可旋转键数：
  3
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.25
• 拓扑面积：
  107
• 氢给体数：
  4
• 氢受体数：
  6

反应信息

• 作为产物：
  描述：

  Kievitone hydrate 生成 水 、 菜豆双氢异黄酮
  参考文献：
  名称：

  从 Fusarium solani f. 中诱导和纯化基维酮水合酶。sp. 菜豆

  摘要：

  茄病镰刀菌 f. sp. 菜豆能够解毒其宿主植物菜豆产生的主要异黄酮植物抗毒素。所涉及的酶活性之一是基维酮水合酶 (KHase)，这是一种分泌糖蛋白，可催化基维酮转化为真菌毒性较低的衍生物基维酮水合物。即使在底物诱导条件下，酶的表达水平也太低而不能令人满意地纯化。因此，测试了几种其他异黄酮作为培养物中的诱导剂。在宿主植物产生的植物抗毒素中，菜豆异黄烷是最好的诱导剂，将分泌酶的水平提高了八倍。用鹰嘴豆产品 biochanin A 处理后，分泌活性增加了 16 倍。在添加 biochanin A 后 9-24 小时观察到最大诱导速率，在此期间还存在诱导剂的几种代谢物。KHase 是从 biochanin A 诱导培养物的滤液中纯化出来的。变性凝胶电泳表明，Mr 47,000 和 49,000 两种物质与活性共纯化。N-末端序列分析表明这两个物种具有相关或相同的多肽部分。与之前确定为约 100,000

  DOI：

  10.1016/0031-9422(90)87088-c

文献信息

• [EN] BACTERIA ENGINEERED FOR CONVERSION OF ETHYLENE TO N-BUTANOL<br/>[FR] BACTÉRIES GÉNÉTIQUEMENT MODIFIÉES POUR LA CONVERSION D'ÉTHYLÈNE EN N-BUTANOL
  申请人：UNIV CALIFORNIA

  公开号：WO2015191611A1

  公开（公告）日：2015-12-17

  The present disclosure provides recombinant bacteria with elevated production of ethanol and/or n-butanol from ethylene. Methods for the production of the recombinant bacteria, as well as for use thereof for production of ethanol and/or n-butanol are also provided.

  本公开提供了具有从乙烯中产生乙醇和/或正丁醇产量升高的重组细菌。还提供了用于生产这些重组细菌以及用于生产乙醇和/或正丁醇的方法。
• The<i>Fusarium solani</i>Gene Encoding Kievitone Hydratase, a Secreted Enzyme that Catalyzes Detoxification of a Bean Phytoalexin
  作者：Daoxin Li

  DOI：10.1094/mpmi-8-0388

  日期：——

  vulgaris (French bean) is the prenylated isoflavonoid, kievitone. The bean pathogen, Fusarium solani f. sp. phaseoli, secretes a glycoenzyme, kievitone hydratase (EC 4.2.1.95), which catalyzes conversion of kievitone to a less toxic metabolite. Among F. solani strains, those that are highly virulent to P. vulgaris also produce kievitone hydratase constitutively, suggesting that the enzyme is a virulence

  菜豆（French bean）产生的抗菌素植物抗毒素中有异戊烯基异黄酮，酮基酮。蚕豆病原菌Fusarium solani f。sp。菜豆油会分泌一种糖酵素，基维酮水合酶（EC 4.2.1.95），可催化基维酮转化为毒性较小的代谢物。在茄形镰刀菌菌株中，对寻常型毕赤酵母具有高毒性的菌株也组成型地产生酮维酮水合酶，表明该酶是一种毒力因子。基于纯化的酶的N末端氨基酸序列，克隆了酮酸水合酶cDNA和基因（khs）。khs和cDNA的同一性通过它们在转基因Neurospora crassa和Emericella nidulans中的表达得以证实。根据基因和cDNA序列，预计khs可编码350个氨基酸的前蛋白，在成熟和分泌过程中从中除去19个氨基酸的N末端转运肽。成熟多肽的预测质量为37 kDa，与纯化酶的电泳估计的47至49 kDa大小相反，这证实了该酶被广泛糖基化。推断的多肽序列具有七个用于N-糖基化的规范位点。F
• Enhancement of plant metabolite production by timed elicitation
  申请人：IDAHO RESEARCH FOUNDATION, INC.

  公开号：EP0378921A2

  公开（公告）日：1990-07-25

  Methods are provided for maximizing the production of secondary metabolites produced from plant cell cultures. The methods involve monitoring the physiological state of the culture cells so that elicitors, which stimulate metabolite production, can be applied at an optimum time. This enhanced production of metabolites following elicitation can be further maximized by the addition of cell viability stabilizers and/or nutrients at the time of elicitation.

  本研究提供了使植物细胞培养物产生的次生代谢物产量最大化的方法。这些方法包括监测培养细胞的生理状态，以便在最佳时间使用刺激代谢物产生的诱导剂。通过在诱导时添加细胞活力稳定剂和/或营养物质，可进一步最大限度地提高诱导后代谢物的产量。
• Plant polynucleotides encoding prenyl proteases
  申请人：BASF Plant Science GmbH

  公开号：EP2157183A1

  公开（公告）日：2010-02-24

  The present invention provides novel polynucleotides encoding plant prenyl protease polypeptides, fragments and homologs thereof. Also provided arc vectors, host cells, antibodies, and recombinant methods for producing said polypeptides. The invention further provides novel polynucleotides encoding plant promoters, polypeptides, fragments and homologs thereof. The invention further relates to methods of applying these novel plant polypeptides to the identification, prevention, and/or conferment of resistence to various plant diseases and/or disorders, particularly drought resistence.

  本发明提供了编码植物前酰蛋白酶多肽、片段及其同源物的新型多核苷酸。还提供了用于生产所述多肽的弧形载体、宿主细胞、抗体和重组方法。本发明进一步提供了编码植物启动子、多肽、片段及其同源物的新型多核苷酸。本发明进一步涉及将这些新型植物多肽应用于鉴定、预防和/或赋予对各种植物病害和/或失调的抗性，特别是抗旱性的方法。
• Cancer cell growth inhibition by black bean (phaseolus vulgaris l) extracts
  申请人：Instituto Tecnologico y de Estudios Superiores de Monterrey

  公开号：EP2311476A2

  公开（公告）日：2011-04-20

  A group of phytochemical compounds extracted from whole germinated or not germinated black beans (Phaseolus vulgaris L) and/or their dry milled fractions such as seed coats or hulls and cotyledons is described. These phytochemicals are classified as phenolics, such as polyphenols, flavonoids, coumarins, and tannins, triterpenes such as saponins, phytosterols and other antioxidant compounds that proved to be effective to decrease cancer proliferation of hormone dependent mammary (MCF-7), hormone independent hepatic (HepG2) and colon (Caco2) cancer cells. They also effectively protect against chemical damage induced with DMBA and furthermore prevented cancer induced by this chemical in Wistar rats and also reduced tumor size when consumed after tumor detection. Methanol, acetone, ethanol, water and mixtures of these solvents were used to obtain the extracts from raw, cooked, and germinated black beans of different varieties, both from the whole grain and the hulls. The black bean malting process produced extracts rich in aglycone forms of the previous mentioned compounds that were more biologically active against MCF-7 cancer cell proliferation. Both raw extracts, C-18 HPLC refined extracts and fractionated extracts may be used in the treatment, prophylaxis, amelioration or defense against a variety of hormone dependent and independent cancers. The extracts and compounds have additional utilities, e.g., lower cholesterol or lower oxidation of LDL and/or inhibit cholesterol synthesis (or the enzyme therefor) and/or reduce liver fibrosis and/or reduce symptoms of menopause and/or stimulate calcium absorption and/or have estrogenic activity such as feminizing estrogenic activity and/or are antioxidants, for instance, as an active ingredient in a nutritional supplement and/or as a food, cosmetic or pharmaceutical antioxidants or colorants.

  本文介绍了从整个发芽或未发芽的黑豆（Phaseolus vulgaris L）和/或其干磨部分（如种皮或种壳和子叶）中提取的一组植物化学物质。这些植物化学物质分为酚类（如多酚、类黄酮、香豆素和单宁）、三萜类（如皂苷）、植物甾醇和其他抗氧化化合物，事实证明它们能有效减少依赖激素的乳腺癌细胞（MCF-7）、不依赖激素的肝癌细胞（HepG2）和结肠癌细胞（Caco2）的癌细胞增殖。它们还能有效抵御 DMBA 诱导的化学损伤，并进一步防止 Wistar 大鼠因这种化学物质而诱发癌症，在检测到肿瘤后食用还能缩小肿瘤大小。研究人员使用甲醇、丙酮、乙醇、水和这些溶剂的混合物，从不同品种的生黑豆、熟黑豆和发芽黑豆（包括整粒黑豆和豆壳）中提取提取物。黑豆发芽过程产生的提取物富含上述化合物的苷元形式，对 MCF-7 癌细胞增殖具有更强的生物活性。生提取物、C-18 HPLC 精炼提取物和分馏提取物均可用于治疗、预防、改善或防御各种激素依赖型和独立型癌症。这些提取物和化合物还具有其他效用，例如，降低胆固醇或降低低密度脂蛋白的氧化和/或抑制胆固醇合成（或其酶）和/或减轻肝纤维化和/或减轻更年期症状和/或刺激钙吸收和/或具有雌激素活性，如雌性化雌激素活性和/或抗氧化剂，例如，作为营养补充剂的活性成分和/或作为食品、化妆品或药品的抗氧化剂或着色剂。