rapidly synthesize sugar phosphates. Compared with chemical synthesis, enzymatic (kinase) routes to sugar phosphates would be attractive for this application. This work focuses upon the development of a high-throughput colorimetric galactokinase (GalK) assay and its application toward probing the substrate specificity and kinetic parameters of Escherichia coli GalK. The demonstrated dinitrosalicylic
体外糖随机化(IVG)技术取决于快速合成糖
磷酸酯的能力。与
化学合成相比,酶促(激酶)途径转化为糖
磷酸酯对于该应用而言将是有吸引力的。这项工作专注于高通量比色半
乳糖激酶(GalK)检测方法的开发及其在探测大肠杆菌GalK的底物特异性和动力学参数方面的应用。证明的二硝基
水杨酸测定法也应普遍适用于多种糖加工酶。[反应:看文字]