3-氨基-8-叠氮-5-苯乙烯-6-苯基菲酚溴化物 | 58880-05-0

• 物质功能分类: 生物化工 - 生化试剂 - 荧光成像
• 分子结构分类: 有机化合物 - 有机杂环化合物 - 喹啉及其衍生物
• 中文名称: 3-氨基-8-叠氮-5-苯乙烯-6-苯基菲酚溴化物
• 中文别名: 溴化乙锭单叠氮溴；EMA[单叠氮化溴乙非啶]；溴代叠氮化乙锭；溴化乙锭单叠氮化物
• 英文名称: 8-Azido-5-ethyl-6-phenylphenanthridin-3(5H)-imine--hydrogen bromide (1/1)
• 英文别名: 8-azido-5-ethyl-6-phenylphenanthridin-3-imine;hydrobromide
• CAS: 58880-05-0
• 化学式: C21H18BrN5
• 分子量: 420.3
• InChiKey: GHUXAYLZEGLXDA-UHFFFAOYSA-N

物化性质

• 熔点：
  >200℃
• 溶解度：
  在DMF中可溶
• 最大波长(λmax)：
  462 nm
• 稳定性/保质期：

  常温常压下稳定，避免接触水。

计算性质

• 辛醇/水分配系数(LogP)：
  2.5
• 重原子数：
  27
• 可旋转键数：
  3
• 环数：
  4.0
• sp3杂化的碳原子比例：
  0.1
• 拓扑面积：
  44.3
• 氢给体数：
  1
• 氢受体数：
  4

安全信息

• WGK Germany：
  3

文献信息

• Linear polyethylenimine-sterol conjugates for gene delivery
  申请人：The University of Utah Research Foundation

  公开号：US20040137050A1

  公开（公告）日：2004-07-15

  Linear polyethylenimine was modified with sterols, such as cholesterol, in three different geometries: linear shaped (L), T-shaped (T), and a combined linear- and T-shaped (LT), to result in linear polyethylenimine-sterol conjugates. These conjugates were mixed with nucleic acids to form complexes for delivery of the nucleic acids into cells. Mammalian cells transfected with these complexes showed protein expression levels higher than linear polyethylenimine alone, and twice that of branched polyethylenimine, but without any significant loss in cell viability. Methods of making these compositions and methods of using them for gene delivery are also described.

  线性聚乙烯亚胺被固醇（如胆固醇）以三种不同的几何形状进行改性：线性形状（L）、T形状（T）和线性和T形状的组合（LT），从而形成线性聚乙烯亚胺-固醇共轭物。这些共轭物与核酸混合形成复合物，用于将核酸传递到细胞中。转染这些复合物的哺乳动物细胞显示出比单独使用线性聚乙烯亚胺更高的蛋白表达水平，且比支化聚乙烯亚胺高出一倍，但没有任何显著的细胞存活率损失。还描述了制备这些组合物的方法以及将其用于基因传递的方法。
• Use of phenanthridium derivatives for distinguishing between intact and membrane-compromised cells using molecular nucleic acid-based techniques
  申请人：Montana State University

  公开号：EP2495338A1

  公开（公告）日：2012-09-05

  The present invention provides novel chemicals and methods for selectively excluding DNA of dead cells from a mixture containing live and dead cells from molecular detection.

  本发明提供了从含有活细胞和死细胞的混合物中选择性地排除死细胞 DNA 以进行分子检测的新型化学物质和方法。
• Hydrogel particles with tunable optical properties and methods for using the same
  申请人：Slingshot Biosciences, Inc.

  公开号：US10753846B2

  公开（公告）日：2020-08-25

  Hydrogel particles and their use in cytometric applications are described. The hydrogel particles described herein are selectively tunable to have at least one optical property substantially similar to the at least one optical property of a target cell. In this regard, the hydrogel particles provided herein in one aspect, are used as a calibration reagent for the detection of a target cell in a sample.

  本文描述了水凝胶微粒及其在细胞测量应用中的使用。本文所述的水凝胶微粒可选择性地调节，使其至少具有一种与目标细胞的至少一种光学特性基本相似的光学特性。在这方面，本文提供的水凝胶微粒在一个方面可用作校准试剂，用于检测样品中的目标细胞。
• USE OF PHENANTHRIDIUM DERIVATIVES FOR DISTINGUISHING BETWEEN INTACT AND MEMBRANE-COMPROMISED CELLS USING MOLECULAR NUCLEIC ACID-BASED TECHNIQUES
  申请人：Montana State University

  公开号：EP2004843A2

  公开（公告）日：2008-12-24
• METHODS OF ISOLATING AND PURIFYING NUCLEIC ACID-BINDING BIOMOLECULES AND COMPOSITIONS INCLUDING SAME
  申请人：Cytocure, Inc.

  公开号：EP2126075A2

  公开（公告）日：2009-12-02