9-(2,3-dideoxy-3-C-methyl-β-D-erythro-pentofuranosyl)guanine | 1058243-89-2

• 分子结构分类: 有机化合物 - 核苷、核苷酸和类似物 - 嘌呤核苷
• 英文名称: 9-(2,3-dideoxy-3-C-methyl-β-D-erythro-pentofuranosyl)guanine
• 英文别名: 2-amino-9-[(2R,4S,5S)-5-(hydroxymethyl)-4-methyloxolan-2-yl]-1H-purin-6-one
• CAS: 1058243-89-2
• 化学式: C₁₁H₁₅N₅O₃
• 分子量: 265.272
• InChiKey: BEODESUKJMHEJO-RRKCRQDMSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -0.6
• 重原子数：
  19
• 可旋转键数：
  2
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.55
• 拓扑面积：
  115
• 氢给体数：
  3
• 氢受体数：
  4

反应信息

• 作为产物：
  描述：

  9-(5-benzoyl-2,3-dideoxy-3-C-methyl-β-D-erythro-pentofuranosyl)-N²-acetylguanine 在 氨 作用下， 以 甲醇 为溶剂， 反应 12.0h， 以83%的产率得到9-(2,3-dideoxy-3-C-methyl-β-D-erythro-pentofuranosyl)guanine
  参考文献：
  名称：

  3'-脱氧-3'-C-甲基核苷衍生物的合成。

  摘要：

  合成带有五个天然存在的核酸碱基的2'，3'-二脱氧-3'-C-甲基核苷。另外，制备了带有5-氨基咪唑-4-羧酰胺以及1,2,4-三唑-3-羧酰胺部分的3'-脱氧-3'-C-甲基核苷类似物。还完成了相应的2'，3'-二脱氧-3'-C-甲基三唑衍生物的合成。通过从它们的3'-脱氧-3'-C-甲基母体核糖核苷进行自由基脱氧制备二脱氧核苷衍生物。当在细胞培养实验中评估其抗病毒活性时，这些化合物均未显示任何显着的抗病毒活性。

  DOI：

  10.1016/j.bmc.2008.06.011

文献信息

• [EN] LANGERIN+ CELL TARGETING<br/>[FR] CIBLAGE DE CELLULES DE LANGERINE+
  申请人：MAX PLANCK GESELLSCHAFT

  公开号：WO2019141731A1

  公开（公告）日：2019-07-25

  The present invention relates to the use of a vehicle for specific molecular targeting of Langerin+ cells, wherein the vehicle is capable of specifically binding to a Langerin+ cell, said vehicle comprising (a) at least one carrier and (b) at least one saccharide moiety-based conjugate for a targeted cargo delivery into a Langerin+ cell, as well as pharmaceutical compositions and uses comprising the inventive vehicle.

  本发明涉及使用一种车辆来针对Langerin+细胞进行特定分子靶向，其中该车辆能够特异性地结合到Langerin+细胞，所述车辆包括（a）至少一种载体和（b）至少一种基于糖苷基的共轭物，用于将目标载荷传递到Langerin+细胞内，以及包括该创新车辆的药物组合物和用途。
• Method for performing molecular reactions by using immiscible intermediate fluids
  申请人：Koninklijke Philips Electronics N.V.

  公开号：EP2502674A1

  公开（公告）日：2012-09-26

  The present invention relates to a method for performing molecular reactions in a device comprising the steps of (a) introducing one or more reagent solution(s) and an immiscible intermediate fluid into the device, wherein the device comprises a substrate, on which chemically or biochemically recognizable entities are immobilized; (b) performing molecular reactions between the immobilized chemically or biochemically recognizable entities and the reagent solution(s); or on the immobilized chemically or biochemically recognizable entities in the presence of the reagent solution(s); (c) displacing the reagent solution(s) present on the substrate by the immiscible intermediate fluid; (d) separating the immiscible intermediate fluid and the reagent solution(s); and reusing the reagent solution(s) and/or immiscible intermediate fluid for one or more repetitions of steps (a) to (e). The invention further relates to a device for performing a molecular reaction, comprising a reaction zone, reservoirs and liquid connections and a collection and regeneration zone wherein the immiscible intermediate fluid and the reagent solution(s) are separable by gravitational separation; or a redirection and distribution module, wherein the immiscible intermediate fluid and reagent solution(s) are separated. The invention also relates to the use of an immiscible intermediate fluid for displacing a reagent solution present in a reaction zone in a microfluidic device, as well as the use of a corresponding device for performing a sequencing reaction or a nucleic acid synthesis reaction.

  本发明涉及一种在装置中进行分子反应的方法，该方法包括以下步骤：(a) 将一种或多种试剂溶液和不溶性中间流体引入装置，其中装置包括基底，在基底上固定化学或生物化学可识别实体；(b) 在固定的化学或生物化学可识别实体和试剂溶液之间进行分子反应；或在试剂溶液存在的情况下，在固定化的化学或生化可识别实体上进行分子反应； (c) 用不溶中间液置换存在于基质上的试剂溶液； (d) 分离不溶中间液和试剂溶液；以及将试剂溶液和/或不溶中间液重复用于步骤(a)至(e)的一次或多次重复。本发明还涉及一种用于进行分子反应的装置，该装置包括反应区、储液器和液体连接装置以及收集和再生区，其中不相溶中间液和试剂溶液可通过重力分离；或一个重新定向和分配模块，其中不相溶中间液和试剂溶液可分离。本发明还涉及使用不相溶中间液置换微流控装置反应区中的试剂溶液，以及使用相应装置进行测序反应或核酸合成反应。
• Devices and methods for efficient capture of nucleic acids
  申请人：Koninklijke Philips Electronics N.V.

  公开号：EP2532754A1

  公开（公告）日：2012-12-12

  The present invention relates a device for the efficient binding of nucleic acids on a microarray, comprising a reaction zone comprising a microarray, and a capture zone comprising a porous membrane substrate, wherein the capture zone is capable of specifically capturing sense strands of target molecules, whereas complementary antisense strands are captured in the reaction zone, or vice versa. The invention further envisages temperature regulating units in the device allowing to bring or keep the capture at a temperature not allowing hybridizing or binding of nucleic acid(s) to the capture molecules or to a temperature suitable for nucleic acid hybridization. The invention also relates to a method of efficiently binding nucleic acids on a microarray, comprising introducing a medium containing one or more target molecules in denatured form into a capture zone of a device of the present invention, performing an interaction reaction between the target molecules and immobilized capture molecules in said capture zone, and transporting not bound target molecule strands to a reaction zone comprising a microarray, thereby allowing an interaction between the target molecule strands and immobilized capture molecules on the microarray. In a further aspect the invention relates to the use of such a device for enriching target molecules, specifically selecting a target molecules, expression analysis, comparative genomic hybridization, the detection of SNPs, or for microarray-based genomic selection-based sequencing.

  本发明涉及一种在微阵列上有效结合核酸的装置，包括一个由微阵列组成的反应区和一个由多孔膜基底组成的捕获区，其中捕获区能够特异性地捕获目标分子的有义链，而互补的反义链则被捕获在反应区，反之亦然。本发明进一步设想了装置中的温度调节单元，可将捕获温度保持在不允许核酸与捕获分子杂交或结合的温度或适合核酸杂交的温度。本发明还涉及一种在微阵列上有效结合核酸的方法，包括将含有一种或多种变性靶分子的介质引入本发明装置的捕获区，在所述捕获区进行靶分子与固定化捕获分子之间的相互作用反应，并将未结合的靶分子链输送到包含微阵列的反应区，从而使靶分子链与微阵列上的固定化捕获分子相互作用。本发明的另一个方面涉及将这种装置用于富集目标分子、特别是选择目标分子、表达分析、比较基因组杂交、SNPs 检测或基于微阵列的基因组选择测序。
• Long rigid spacers to enhance binding kinetics in immunoassays
  申请人：Koninklijke Philips Electronics N.V.

  公开号：EP2594942A1

  公开（公告）日：2013-05-22

  The present invention relates to a device for detecting a target molecule within a sample comprising a sample container for the measurement of the target molecule within a sample, a first particle, wherein said first particle is functionalized with a first binding molecule capable of specifically binding to said target molecule, and a surface structure comprising a second binding molecule, wherein said surface structure covers a flat sensor or is present on a second particle, wherein said first particle is capable of binding said second binding molecule of the surface structure directly or indirectly; wherein said first and/or second binding molecule is directly or indirectly attached to the particle surface of said first and/or second particle and/or the flat sensor surface via a long and rigid linker molecule; wherein the length and the consistency of said linker molecule is selected such as to result in an average extension length of said linker of more than 60 nm; and wherein the number of particle clusters or of bound particles is directly or inversely related to the amount of target molecules present in the sample. In a further aspect the present invention relates to a method of detecting the presence or amount of a target molecule within a sample. The present invention also describes the use of a particle according to the invention for detecting a target molecule within a sample.

  本发明涉及一种用于检测样品中目标分子的装置，该装置包括用于测量样品中目标分子的样品容器、第一粒子（其中所述第一粒子被官能化，具有能够与所述目标分子特异性结合的第一结合分子）和包括第二结合分子的表面结构（其中所述表面结构覆盖平面传感器或存在于第二粒子上），其中所述第一粒子能够直接或间接结合表面结构的所述第二结合分子；其中，所述第一和/或第二结合分子通过长而坚硬的连接分子直接或间接地连接到所述第一和/或第二粒子的粒子表面和/或平面传感器表面；其中，所述连接分子的长度和一致性经过选择，以使所述连接分子的平均延伸长度超过 60 nm；以及其中，粒子簇或结合粒子的数量与样品中存在的目标分子的数量直接或成反比。本发明的另一个方面涉及一种检测样品中目标分子的存在或数量的方法。本发明还描述了根据本发明的粒子用于检测样品中目标分子的用途。
• Particle repulsion to enhance surface contact in magnetic particle immunoassays
  申请人：Koninklijke Philips Electronics N.V.

  公开号：EP2594940A1

  公开（公告）日：2013-05-22

  The present invention relates to a device for detecting a target molecule within a sample comprising a sample container for the measurement of the target molecule within a sample, a magnetic particle, wherein said particle is functionalized with a first binding molecule capable of specifically binding to said target molecule, wherein said first binding molecule is attached to the particle, and a repulsive surface structure, which is directly attached to the surface of said particle, wherein said repulsive surface structure covers the surface of the magnetic particle so as to result in a specific net charge and/or steric repulsion of the magnetic particle, and a sensor surface comprising a second binding molecule, wherein said magnetic particles are capable of binding said second binding molecule of the sensor surface directly or indirectly; wherein the number of bound particles is directly or inversely related to the amount of target molecules present in the sample; and wherein said repulsive surface structure conveys an electrostatic and/or steric pushing effect on said magnetic particles towards said sensor surface.

  本发明涉及一种用于检测样品中目标分子的装置，该装置包括一个用于测量样品中目标分子的样品容器、一个磁性颗粒，其中所述颗粒用能够特异性结合所述目标分子的第一结合分子功能化，其中所述第一结合分子附着在颗粒上，以及一个斥性表面结构、其中，所述斥力表面结构覆盖磁性粒子表面，从而导致磁性粒子的特定净电荷和/或立体斥力，以及传感器表面，该传感器表面包含第二结合分子，其中，所述磁性粒子能够直接或间接结合传感器表面的所述第二结合分子；其中，结合颗粒的数量与样品中存在的目标分子的数量直接或成反比；以及其中，所述斥性表面结构对所述磁性颗粒向所述传感器表面传递静电和/或立体推动效应。