rCyd.rCyd.rAdo

• 分子结构分类: 有机化合物 - 核苷、核苷酸和类似物 - 嘌呤核苷
• 英文名称: rCyd.rCyd.rAdo
• 英文别名: 4-amino-1-[(2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]pyrimidin-2-one;(2R,3R,4S,5R)-2-(6-aminopurin-9-yl)-5-(hydroxymethyl)oxolane-3,4-diol
• 化学式: C28H39N11O14
• 分子量: 753.7
• InChiKey: UOAZXKZIOLKOOJ-JRJYXWDASA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -7.11
• 重原子数：
  53
• 可旋转键数：
  6
• 环数：
  7.0
• sp3杂化的碳原子比例：
  0.54
• 拓扑面积：
  397
• 氢给体数：
  12
• 氢受体数：
  18

文献信息

• MUTATED TRNA FOR CODON EXPANSION
  申请人：CHUGAI SEIYAKU KABUSHIKI KAISHA

  公开号：EP3904568A1

  公开（公告）日：2021-11-03

  In some embodiments, the present disclosure relates to mutated tRNAs in which the first letter of the anticodon has been substituted to lysidine or agmatidine, and translation systems containing the mutated tRNAs. In a specific embodiment, the present disclosure provides mutated tRNAs capable of selectively translating codon NNA. In another embodiment, the present disclosure provides translation systems capable of translating two or three types of amino acids from a single codon box. In still another embodiment, the present disclosure provides novel methods for synthesizing lysidine diphosphate, agmatidine diphosphate, and derivatives thereof.

  在某些实施方案中，本公开涉及反密码子的第一个字母被替换为赖氨酸或琼脂苷的突变 tRNA，以及含有该突变 tRNA 的翻译系统。在一个具体的实施方案中，本公开提供了能够选择性翻译密码子 NNA 的突变 tRNA。在另一个实施方案中，本公开提供了能够从单个密码子盒翻译两种或三种氨基酸的翻译系统。在另一个实施方案中，本公开提供了合成二磷酸赖氨酸、二磷酸阿加匹定及其衍生物的新方法。
• COMPOSITION FOR TRANSLATION, AND METHOD FOR PRODUCING PEPTIDE
  申请人：CHUGAI SEIYAKU KABUSHIKI KAISHA

  公开号：EP4026906A1

  公开（公告）日：2022-07-13

  An objective of the present invention is to provide novel means that allow reducing the rate of mistranslation into unintended amino acids due to misreading of codons. In a number of embodiments, the present disclosure provides compositions for translation which contain a tRNA having a specific combination of bases at positions 32, 33, 37, and 38 (tRNA numbering rule) in the tRNA. According to a specific embodiment of the present disclosure, the rate of introduction of unintended amino acids due to codon misreading can be reduced.

  本发明的一个目的是提供新的方法，以减少由于密码子的误读而导致的误翻译成非预期氨基酸的比率。在一些实施方案中，本发明公开了用于翻译的组合物，该组合物含有在 tRNA 的 32、33、37 和 38 位（tRNA 编号规则）具有特定碱基组合的 tRNA。根据本公开的一个具体实施例，可以降低由于密码子误读而导致的非预期氨基酸的引入率。
• Compositions and methods for making selenocysteine containing polypeptides
  申请人：Yale University

  公开号：US10023893B2

  公开（公告）日：2018-07-17

  Non-naturally occurring tRNASec and methods of using them for recombinant expression of proteins engineered to include one or more selenocysteine residues are disclosed. The non-naturally occurring tRNASec can be used for recombinant manufacture of selenocysteine containing polypeptides encoded by mRNA without the requirement of an SECIS element. In some embodiments, selenocysteine containing polypeptides are manufactured by co-expressing a non-naturally occurring tRNASec a recombinant expression system, such as E. coli, with SerRS, EF-Tu, SelA, or PSTK and SepSecS, and an mRNA with at least one codon that recognizes the anticodon of the non-naturally occurring tRNASec.

  本发明公开了非天然存在的 tRNASec 及其用于重组表达包含一个或多个硒半胱氨酸残基的工程蛋白的方法。非天然存在的 tRNASec 可用于重组制造由 mRNA 编码的含硒半胱氨酸的多肽，而不需要 SECIS 元件。在某些实施方案中，通过在重组表达系统（如大肠杆菌）中共表达非天然存在的 tRNASec 与 SerRS、EF-Tu、SelA 或 PSTK 和 SepSecS，以及至少有一个密码子能识别非天然存在的 tRNASec 的反密码子的 mRNA，来制造含硒半胱氨酸的多肽。
• Compositions and Methods for Making Selenocysteine Containing Polypeptides
  申请人：Soll Dieter

  公开号：US20140154744A1

  公开（公告）日：2014-06-05

  Non-naturally occurring tRNA Sec and methods of using them for recombinant expression of proteins engineered to include one or more selenocysteine residues are disclosed. The non-naturally occurring tRNA Sec can be used for recombinant manufacture of selenocysteine containing polypeptides encoded by mRNA without the requirement of an SECIS element. In some embodiments, selenocysteine containing polypeptides are manufactured by co-expressing a non-naturally occurring tRNA Sec a recombinant expression system, such as E. coli, with SerRS, EF-Tu, SelA, or PSTK and SepSecS, and an mRNA with at least one codon that recognizes the anticodon of the non-naturally occurring tRNA Sec .
• COMPOSITIONS AND METHODS FOR MAKING SELENOCYSTEINE CONTAINING POLYPEPTIDES
  申请人：Yale University

  公开号：US20170029858A1

  公开（公告）日：2017-02-02

  Non-naturally occurring tRNA Sec and methods of using them for recombinant expression of proteins engineered to include one or more selenocysteine residues are disclosed. The non-naturally occurring tRNA Sec can be used for recombinant manufacture of selenocysteine containing polypeptides encoded by mRNA without the requirement of an SECIS element. In some embodiments, selenocysteine containing polypeptides are manufactured by co-expressing a non-naturally occurring tRNA Sec a recombinant expression system, such as E. coli , with SerRS, EF-Tu, SelA, or PSTK and SepSecS, and an mRNA with at least one codon that recognizes the anticodon of the non-naturally occurring tRNA Sec .