申请人:3M INNOVATIVE PROPERTIES COMPANY
公开号:US10640743B2
公开(公告)日:2020-05-05
A thin film culture device for detecting yeast and mold microorganisms in a sample is provided. The culture device comprises a body comprising a self-supporting substrate having a first major surface and a second major surface; a first adhesive composition disposed on a portion of the first major surface of the substrate; a substantially dry, cold-water-soluble first hydrogel-forming composition adhered to the first adhesive composition; and a plurality of indicator agents. The plurality of indicator agents comprises three indicator agents for detecting distinct glycosidase enzyme activities, an indicator agent for detecting an alkyl esterase enzyme activity, and an indicator agent for detecting a phosphatase enzyme activity, wherein each of the plurality of indicator agents comprises a detectable reporter group. A method of using the culture device is also provided.
提供了一种用于检测样品中酵母和霉菌微生物的薄膜培养装置。该培养装置包括一个主体,该主体包括具有第一大表面和第二大表面的自支撑基底;设置在基底第一大表面部分上的第一粘合剂组合物;粘附在第一粘合剂组合物上的基本干燥、冷水可溶的第一水凝胶形成组合物;以及多种指示剂。多种指示剂包括三种用于检测不同糖苷酶酶活性的指示剂、一种用于检测烷基酯酶酶活性的指示剂和一种用于检测磷酸酶酶活性的指示剂,其中多种指示剂中的每一种都包括可检测的报告基团。还提供了一种使用该培养装置的方法。