L-丙氨酰对硝基苯胺盐酸盐 | 31796-55-1

• 物质功能分类: 生物化工 - 氨基酸及其衍生物 - 丙氨酸类衍生物
• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 羧酸及其衍生物
• 中文名称: L-丙氨酰对硝基苯胺盐酸盐
• 中文别名: 4-硝基苯胺盐酸盐-L-丙氨酸；L-丙氨酸4-硝基酰苯胺盐酸盐；H-Ala-pNA・HCl；β-丙氨酸甲酯.HCl
• 英文名称: H-Ala-pNA*HCl
• 英文别名: H-Ala-pNA hydrochloride；L-alanine-4-nitroanilide hydrochloride；[(2S)-1-(4-nitroanilino)-1-oxopropan-2-yl]azanium;chloride
• CAS: 31796-55-1
• 化学式: C₉H₁₁N₃O₃*ClH
• 分子量: 245.666
• InChiKey: YEXRLSXNWLNHQR-RGMNGODLSA-N

物化性质

• 溶解度：
  H2O：50 mg/mL，澄清，黄色

计算性质

• 辛醇/水分配系数(LogP)：
  1.3
• 重原子数：
  16
• 可旋转键数：
  2
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.22
• 拓扑面积：
  101
• 氢给体数：
  3
• 氢受体数：
  4

安全信息

• 安全说明：
  S22,S24/25
• WGK Germany：
  3
• 海关编码：
  2924299090

制备方法与用途

用途：蛋白酶底物

反应信息

• 作为反应物：
  描述：

  methyl (2-aminobenzoyl)phenylalaninate 、 L-丙氨酰对硝基苯胺盐酸盐 在 N-甲基吗啉 、 chymotrypsin 作用下， 以 N,N-二甲基甲酰胺 为溶剂， 反应 13.0h， 以70%的产率得到2-amino-N-((S)-1-(((S)-1-((4-nitrophenyl)amino)-1-oxopropan-2-yl)amino)-1-oxo-3-phenylpropan-2-yl)benzamide
  参考文献：
  名称：

  Chemoenzymatic synthesis of new fluorogenous substrates for cysteine proteases of the papain family

  摘要：

  A chemoenzymatic synthesis was developed for new highly specific fluorogenic substrates for cysteine proteases of the papain family, Abz-Phe-Ala-pNA (I) and Glp-Phe-Ala-Amc (II) (Abz, pNA, Glp, and Amc are o-aminobenzoyl,p-nitroanilide, pyroglutamyl, and 4-amino-7-methylcoumaride, respectively). Substrate (I) was obtained in an aqueous-organic medium using native chymotrypsin. Substrate (II) was synthesized in DMF-MeCN by the treatment with chymotrypsin and subtilisin Carlsberg immobilized on polyvinyl alcohol cryogel. Hydrolysis of substrate (I) with papain, ficin, and bromelain was accompanied by a 15-fold increase in fluorescence intensity, and that of substrate (II), by a change in the fluorescence spectrum. Unambiguity of enzymatic hydrolysis of the substrates after the Ala residue was shown. The specific activity of the substrate hydrolysis with papain, bromelain, and ficin and was determined. Papain showed the greatest activity for both substrates. The activity of all proteases under study was essentially higher for substrate (II), than for substrate (I). The lowest detectable papain concentrations were 2.4 x 10(-10) M for (I) and 1.2 x 10(-11) M for (II). A high selectivity of cysteine proteases for Glp-Phe-Ala-Ame was established.

  DOI：

  10.1134/s1068162008030151

文献信息

• Methods and compounds for inhibiting beta-amyloid peptide release and/or its synthesis
  申请人：——

  公开号：US20030229024A1

  公开（公告）日：2003-12-11

  Disclosed are compounds which inhibit &bgr;-amyloid peptide release and/or its synthesis, and, accordingly, have utility in treating Alzheimer's disease. Also disclosed pharmaceutical compositions comprising a compound which inhibits &bgr;-amyloid peptide release and/or its synthesis as well as methods for treating Alzheimer's disease both prophylactically and therapeutically with such pharmaceutical compositions.

  本发明涉及一种抑制&bgr;-淀粉样肽释放和/或合成的化合物，因此具有治疗阿尔茨海默病的用途。还公开了药物组合物，包括抑制&bgr;-淀粉样肽释放和/或合成的化合物，以及使用这样的药物组合物进行预防和治疗阿尔茨海默病的方法。
• Novel Methods for Bone Treatment by Modulating an Arachidonic Acid Metabolic or Signaling Pathway
  申请人：O'Connor James Patrick

  公开号：US20110105959A1

  公开（公告）日：2011-05-05

  Methods for promoting osteogenesis to accelerate or enhance bone fracture healing, treat bone defects, and enhance bone formation are disclosed. The methods rely on in vivo or ex vivo modulation of an arachidonic acid metabolic or signaling pathway in general, and, in particular, utilize 5-lipoxygenase inhibitors, leukotriene A4 hydrolase inhibitors, and/or leukotriene B4 receptor antagonists. These molecules can be delivered alone or in combination with one or more agents that inhibit bone resorption, regulate calcium resorption from bone, enhance bone accumulation, enhance bone formation, induce bone formation, impair growth of microorganisms, reduce inflammation, and/or reduce pain.

  公开了促进成骨作用以加速或增强骨折愈合、治疗骨缺陷和增强骨形成的方法。这些方法依赖于体内或体外调节花生四烯酸代谢或信号通路的总体，特别是利用5-脂氧合酶抑制剂、白三烯A4水解酶抑制剂和/或白三烯B4受体拮抗剂。这些分子可以单独或与一个或多个抑制骨吸收、调节从骨中钙吸收、增强骨积累、增强骨形成、诱导骨形成、抑制微生物生长、减少炎症和/或减轻疼痛的剂量联合使用。
• Dried spore germinative compound mixtures
  申请人：Envera, LLC

  公开号：US10308909B2

  公开（公告）日：2019-06-04

  In one aspect, the present invention is directed to a dried intimate mixture comprising a bacteria spore and a germinative compound, and methods for preparing the intimate mixture. In another aspect, this invention is directed to a composition comprising such an intimate mixture. The invention also relates to methods for increasing the germination, growth, metabolism, and/or enzyme activity of a bacteria spore comprising preparing an intimate mixture of a bacteria spore and a germinative compound.

  一方面，本发明涉及一种由细菌孢子和发芽化合物组成的干燥亲密混合物，以及制备这种亲密混合物的方法。另一方面，本发明涉及一种包含这种亲密混合物的组合物。本发明还涉及提高细菌孢子的发芽、生长、新陈代谢和/或酶活性的方法，包括制备细菌孢子和发芽化合物的亲密混合物。
• Cell culture system
  申请人：PRESIDENT AND FELLOWS OF HARVARD COLLEGE

  公开号：US10472612B2

  公开（公告）日：2019-11-12

  The embodiments of the invention described herein relate to systems and methods for culturing and/or maintaining intestinal cells, tissues and/or organoids in vitro. The cells, tissues and/or organoids cultured according to the methods and systems described herein can mimic or reproduce natural intestinal epithelial structures and behavior as well as support co-culture of intestinal microflora.

  本文所述的本发明的实施方案涉及体外培养和/或维持肠细胞、组织和/或器 官的系统和方法。根据本文所述方法和系统培养的细胞、组织和/或器质体可模仿或再现天然肠道上皮结构和行为，并支持肠道微生物菌群的共培养。
• Analytical system based upon spore germination
  申请人：——

  公开号：US20030008337A1

  公开（公告）日：2003-01-09

  An analytical system for rapid detection and identification of different analytes directly from a test sample by mixing test material with a germinogenic source and enzyme-free spores, allowing the mixture to stand for a time to allow analyte-induced spore germination and subsequent de novo activity of an enzyme capable of producing a germinant in the presence of the germinogenic source and detecting the presence of a germination-derived product. The germinant which is formed promotes further spore germination with concomitant additional de novo enzyme synthesis or activation which results in a propagating cascade of analyte-independent germination after which a germination-derived product can be easily detected. The technique is particularly efficient to conduct thousands of parallel assays in an array of microscopic wells.

  一种直接从测试样品中快速检测和鉴定不同分析物的分析系统，将测试材料与发芽源和无酶孢子混合，使混合物静置一段时间，使分析物诱导的孢子发芽，随后在发芽源存在的情况下能够产生发芽体的酶的新生活性，并检测发芽衍生产物的存在。形成的萌发剂可促进孢子进一步萌发，同时产生额外的新酶合成或激活，从而产生一连串不依赖分析物的萌发过程，之后便可轻松检测到萌发衍生产物。该技术尤其适用于在显微孔阵列中进行数千次平行检测。