sucrose 6'-phosphate | 4549-10-4

• 分子结构分类: 有机化合物 - 有机氧化合物
• 英文名称: sucrose 6'-phosphate
• 英文别名: (O⁶-phosphono-β-D-fructofuranosyl)-α-D-glucopyranoside；(O⁶-Phosphono-β-D-fructofuranosyl)-α-D-glucopyranosid；[(2R,3S,4S,5S)-3,4-dihydroxy-5-(hydroxymethyl)-5-[(2R,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyoxolan-2-yl]methyl dihydrogen phosphate
• CAS: 4549-10-4
• 化学式: C₁₂H₂₃O₁₄P
• 分子量: 422.28
• InChiKey: PJTTXANTBQDXME-UGDNZRGBSA-N

物化性质

• 沸点：
  810.7±75.0 °C(Predicted)
• 密度：
  1.90±0.1 g/cm3(Temp: 20 °C; Press: 760 Torr)(Predicted)

计算性质

• 辛醇/水分配系数(LogP)：
  -5.3
• 重原子数：
  27
• 可旋转键数：
  7
• 环数：
  2.0
• sp3杂化的碳原子比例：
  1.0
• 拓扑面积：
  236
• 氢给体数：
  9
• 氢受体数：
  14

反应信息

• 作为产物：
  描述：

  蔗糖 在 吡啶 、 calcium sulfate 、 对甲苯磺酸 、 三氯氧磷 作用下， 以 N,N-二甲基甲酰胺 、 乙腈 为溶剂， 反应 3.75h， 生成 sucrose 6'-phosphate
  参考文献：
  名称：

  A new synthesis of sucrose 6′-phosphate

  摘要：

  Sucrose 6'-phosphate (3) is the key intermediate for sucrose (1) synthesis in plants [1]. It has recently become commercially available at ca. $1500/g (Sigma). The only chemical synthesis is that of Buchanan et al. [2]. This six-step procedure, while unambiguous, gives an overall yield of only ca. 6%. We describe here a simplified route (2 steps) with an unoptimized yield of ca. 15%. Our strategy was to use a phosphorylation reagent selective for primary hydroxyl groups and thus to avoid the necessity for blocking all of the secondary ones. Sowa and Ouchi [3] described a suitable system which they used very effectively for the synthesis of 5'-nucleotides from unprotected nucleosides. We applied this reagent to 2,1':4,6-di-O-isopropylidenesucrose (2) [4] in which the only unprotected primary hydroxyl group is that at the 6'-position (Scheme 1). The identity of the product was established by comparison of its rotation with the literature value and by the correspondence of its 1H and 13C NMR spectra with those of an authentic sample synthesized by Buchanan's method (Sigma) 1H assignments were made with the help of the assignments of du Penhoat et al. [5] for sucrose and the results of a one-bond H-C COSY experiment (Fig. 1). The 13C spectrum showed that all of the resonances were shifted downfield by ca. 0.5 ppm as compared with sucrose [6] except for the C-5' doublet which was shifted upfield by 0.5 ppm and the C-6' doublet which was shifted downfield by 2.2 ppm (Table 1).

  DOI：

  10.1016/0008-6215(94)00003-x

文献信息

• Sucrose phosphate synthase (SPS), its process for preparation, its cDNA, and utilization of cDNA to modify the expression of SPS in the plant cells
  申请人：ROUSSEL UCLAF

  公开号：EP0466995A2

  公开（公告）日：1992-01-22

  Sucrose phosphate synthase (SPS), its process for preparation, its cDNA, and utilization of cDNA to modify the expression of SPS in the plant cells.

  蔗糖磷酸合成酶（SPS）、其制备过程、其 cDNA 以及利用 cDNA 改变 SPS 在植物细胞中的表达。
• Sucrose phosphate synthase (SPS), its process for preparation, its cDNA, and utilisation of cDNA to modify the expression of SPS in the plant cells
  申请人：ROUSSEL UCLAF

  公开号：EP0718401A1

  公开（公告）日：1996-06-26

  Sucrose phosphate synthase (SPS), its process for preparation, its cDNA, and utilization of cDNA to modify the expression of SPS in plant cells.

  蔗糖磷酸合成酶（SPS）、其制备过程、其 cDNA 以及利用 cDNA 改变 SPS 在植物细胞中的表达。
• Sucrose phosphate synthase (sps), its process for preparation, its cdna and utilization of cdna to modify the expression if sps in the plant cells
  申请人：ROUSSEL-UCLAF

  公开号：EP0807685A2

  公开（公告）日：1997-11-19

  Sucrose phosphate synthase (SPS), its process for preparation, its cDNA, and utilization of cDNA to modify the expression of SPS in the plant cells.

  蔗糖磷酸合成酶（SPS）、其制备过程、其 cDNA 以及利用 cDNA 改变 SPS 在植物细胞中的表达。
• Probe compound for detecting and isolating enzymes and means and methods using the same
  申请人：Helmholtz-Zentrum für Infektionsforschung GmbH

  公开号：EP2230312A1

  公开（公告）日：2010-09-22

  The present invention relates to a probe compound that can comprise any substrate or metabolite of an enzymatic reaction in addition to an indicator component, such as, for example, a fluorescence dye, or the like. Moreover, the present invention relates to means for detecting enzymes in form of an array, which comprises any number of probe compounds of the invention which each comprise a different metabolite of interconnected metabolites representing the central pathways in all forms of life. Moreover, the present invention relates to a method for detecting enzymes involving the application of cell extracts or the like to the array of the invention which leads to reproducible enzymatic reactions with the substrates. These specific enzymatic reactions trigger the indicator (e.g. a fluorescence signal) and bind the enzymes to the respective cognate substrates. Moreover, the invention relates to means for isolating enzymes in form of nanoparticles coated with the probe compound of the invention. The immobilisation of the cognate substrates or metabolites on the surface of nanoparticles by means of the probe compounds allows capturing and isolating the respective enzyme, e.g. for subsequent sequencing.

  本发明涉及一种探针化合物，它可以包括酶反应的任何底物或代谢物，此外还包括指示成分，例如荧光染料或类似物。此外，本发明还涉及以阵列形式检测酶的方法，该阵列由任意数量的本发明探针化合物组成，每种探针化合物由代表所有生命形式中中心途径的相互关联的代谢物中的不同代谢物组成。此外，本发明还涉及一种检测酶的方法，该方法涉及将细胞提取物或类似物应用于本发明的阵列，从而导致与底物发生可重复的酶反应。这些特定的酶反应会触发指示剂（如荧光信号），并将酶与各自的同源底物结合。此外，本发明还涉及以涂覆有本发明探针化合物的纳米颗粒形式分离酶的方法。通过探针化合物将同源底物或代谢物固定在纳米颗粒表面，可以捕获和分离相应的酶，例如用于后续测序。
• Compositions comprising promoter sequences and methods of use
  申请人：NORTH CAROLINA STATE UNIVERSITY

  公开号：EP2361983A1

  公开（公告）日：2011-08-31

  Nucleic acid molecules, fragments and variants thereof having promoter activity are provided in the current invention. The invention also provides vectors containing a nucleic acid molecule of the invention and cells comprising the vectors. Methods for making and using the nucleic acid molecules of the invention are further provided.

  本发明提供了具有启动子活性的核酸分子、片段及其变体。本发明还提供了含有本发明核酸分子的载体和含有该载体的细胞。本发明还提供了制造和使用本发明核酸分子的方法。