6-fluoro-4-((4-((3-morpholinopropyl)amino)phenyl)amino)quinoline-3-carboxylic acid

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 喹啉及其衍生物
• 英文名称: 6-fluoro-4-((4-((3-morpholinopropyl)amino)phenyl)amino)quinoline-3-carboxylic acid
• 英文别名: 6-Fluoro-4-[4-(3-morpholinopropylamino)anilino]quinoline-3-carboxylic acid；6-fluoro-4-[4-(3-morpholin-4-ylpropylamino)anilino]quinoline-3-carboxylic acid
• 化学式: C₂₃H₂₅FN₄O₃
• 分子量: 424.475
• InChiKey: HAVLBPZXTFNRAU-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  1.6
• 重原子数：
  31
• 可旋转键数：
  8
• 环数：
  4.0
• sp3杂化的碳原子比例：
  0.3
• 拓扑面积：
  86.7
• 氢给体数：
  3
• 氢受体数：
  8

反应信息

• 作为产物：
  描述：

  特定基氨基甲酸脂酶 在 lithium hydroxide monohydrate 、 水 、 potassium carbonate 、 对甲苯磺酸 、 三氟乙酸 作用下， 以 四氢呋喃 、 甲醇 、 二氯甲烷 、 N,N-二甲基甲酰胺 为溶剂， 反应 10.0h， 生成 6-fluoro-4-((4-((3-morpholinopropyl)amino)phenyl)amino)quinoline-3-carboxylic acid
  参考文献：
  名称：

  4-Aminoquinoline derivatives as novel Mycobacterium tuberculosis GyrB inhibitors: Structural optimization, synthesis and biological evaluation

  摘要：

  Mycobacterial DNA gyrase B subunit has been identified to be one of the potentially underexploited drug targets in the field of antitubercular drug discovery. In the present study, we employed structural optimization of the reported GyrB inhibitor resulting in synthesis of a series of 46 novel quinoline derivatives. The compounds were evaluated for their in vitro Mycobacterium smegmatis GyrB inhibitory ability and Mycobacterium tuberculosis DNA supercoiling inhibitory activity. The antitubercular activity of these compounds was tested over Mtb H37Rv strain and their safety profile was checked against mouse macrophage RAW 264.7 cell line. Among all, three compounds (23, 28, and 53) emerged to be active displaying IC50 values below 1 mu M against Msm GyrB and were found to be non-cytotoxic at 50 mu M concentration. Compound 53 was identified to be potent GyrB inhibitor with 0.86 +/- 0.16 mu M and an MIC (minimum inhibitory concentration) of 3.3 mu M. The binding affinity of this compound towards GyrB protein was analysed by differential scanning fluorimetry which resulted in a positive shift of 3.3 degrees C in melting temperature (T-m) when compared to the native protein thereby reacertaining the stabilization effect of the compound over protein. (C) 2015 Elsevier Masson SAS. All rights reserved.

  DOI：

  10.1016/j.ejmech.2015.06.032

文献信息

• 4-Aminoquinoline derivatives as novel Mycobacterium tuberculosis GyrB inhibitors: Structural optimization, synthesis and biological evaluation
  作者：Brahmam Medapi、Priyanka Suryadevara、Janupally Renuka、Jonnalagadda Padma Sridevi、Perumal Yogeeswari、Dharmarajan Sriram

  DOI：10.1016/j.ejmech.2015.06.032

  日期：2015.10

  Mycobacterial DNA gyrase B subunit has been identified to be one of the potentially underexploited drug targets in the field of antitubercular drug discovery. In the present study, we employed structural optimization of the reported GyrB inhibitor resulting in synthesis of a series of 46 novel quinoline derivatives. The compounds were evaluated for their in vitro Mycobacterium smegmatis GyrB inhibitory ability and Mycobacterium tuberculosis DNA supercoiling inhibitory activity. The antitubercular activity of these compounds was tested over Mtb H37Rv strain and their safety profile was checked against mouse macrophage RAW 264.7 cell line. Among all, three compounds (23, 28, and 53) emerged to be active displaying IC50 values below 1 mu M against Msm GyrB and were found to be non-cytotoxic at 50 mu M concentration. Compound 53 was identified to be potent GyrB inhibitor with 0.86 +/- 0.16 mu M and an MIC (minimum inhibitory concentration) of 3.3 mu M. The binding affinity of this compound towards GyrB protein was analysed by differential scanning fluorimetry which resulted in a positive shift of 3.3 degrees C in melting temperature (T-m) when compared to the native protein thereby reacertaining the stabilization effect of the compound over protein. (C) 2015 Elsevier Masson SAS. All rights reserved.