4-胺苯苯基-1-硫代-beta-D-半乳糖苷 | 29558-05-2

• 物质功能分类: 生物化工 - 糖类化合物 - 单糖
• 分子结构分类: 有机化合物 - 有机氧化合物
• 中文名称: 4-胺苯苯基-1-硫代-beta-D-半乳糖苷
• 中文别名: 4-氨基苯基-1-硫代-Β-D-半乳糖苷
• 英文名称: p-Aminophenyl 1-thio-β-D-galaktopyranosid
• 英文别名: (2S,3R,4S,5R,6R)-2-(4-aminophenyl)sulfanyl-6-(hydroxymethyl)oxane-3,4,5-triol
• CAS: 29558-05-2
• 化学式: C₁₂H₁₇NO₅S
• 分子量: 287.337
• InChiKey: XCNVFDDRUPMRPU-IIRVCBMXSA-N

物化性质

• 熔点：
  172-173°C
• 沸点：
  587℃
• 密度：
  1.54
• 闪点：
  309℃

计算性质

• 辛醇/水分配系数(LogP)：
  -0.4
• 重原子数：
  19
• 可旋转键数：
  3
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.5
• 拓扑面积：
  142
• 氢给体数：
  5
• 氢受体数：
  7

安全信息

• 安全说明：
  S22,S24/25
• 海关编码：
  2932999099

反应信息

• 作为产物：
  描述：

  4-氨基苯硫酚 、 4-硝基苯基-D-吡喃葡糖苷 在 E₁₅₉Q mutant of thioglycoligase from Dictyoglomus thermophilum 作用下， 以 aq. buffer 为溶剂， 反应 24.0h， 以79%的产率得到4-胺苯苯基-1-硫代-beta-D-半乳糖苷
  参考文献：
  名称：

  一系列硫糖苷的酶法合成：具有有效抗色素沉着特性的熊果苷类似物

  摘要：

  生物催化合成导致制备了一系列熊果苷的硫糖苷类似物。这些原始分子显示出有效的酪氨酸酶抑制特性以及与细胞表面凝集素相互作用的能力。因此，硫原子的存在证明增加了这些糖苷的生物效力。

  DOI：

  10.1002/ejoc.202100672

文献信息

• Functionalized nanotubes
  申请人：Hyperion Catalysis International, Inc.

  公开号：US20040202603A1

  公开（公告）日：2004-10-14

  Graphitic nanotubes, which includes tubular fullerenes (commonly called “buckytubes”) and fibrils, which are functionalized by chemical substitution or by adsorption of functional moieties. More specifically the invention relates to graphitic nanotubes which are uniformly or non-uniformly substituted with chemical moieties or upon which certain cyclic compounds are adsorbed and to complex structures comprised of such functionalized nanotubes linked to one another. The invention also relates to methods for introducing functional groups onto the surface of such nanotubes. The invention further relates to uses for functionalized nanotubes.

  石墨烯纳米管包括管状富勒烯（通常称为“巴基管”）和纤维，它们通过化学取代或吸附功能基团进行功能化。更具体地说，该发明涉及均匀或不均匀取代化学基团或吸附某些环状化合物的石墨烯纳米管，以及由这些功能化纳米管相互连接而成的复杂结构。该发明还涉及将功能基团引入这些纳米管表面的方法。该发明进一步涉及功能化纳米管的用途。
• Complexing of biologically active or functional compounds and methods of preparing and utilizing same
  申请人：ENZO BIOCHEM, INC.

  公开号：EP0123300A2

  公开（公告）日：1984-10-31

  Complexes containing a biologically active or functional compound or compounds are prepared by attaching or fixing a first compound, which may be a biologically active or functional compound, to a solid substrate under conditions such that the active site of said first compound is protected, thereupon derivatizing or combining said first compound with a derivatizing or modifying compound while said first compound is attached to said solid substrate. The resulting complex or complexed first compound is then released from attachment to said solid substrate to yield the complex, said complex comprising said derivatizing compound attached to said first compound, said first compound, upon release from the solid substrate having available its functional or biologically active site. For example, alkaline phosphatase is attached to a solid substrate, such as dextran beads, preferably through a ligand, such as a competitive inhibitor for alkaline phosphatase, e.g. para-aminobenzyl-phosphonic acid (p-ABPA). The dextran beads would be first treated with (p-ABPA) and the alkaline phosphatase brought into contact with the thus-treated dextran beads. The competitive inhibitor p-ABPA serves as a ligand for attachment of the alkaline phosphatase to the dextran beads while at the same time occupying and protecting the active site of the alkaline phosphatase. Thereupon, the thus-attached alkaline phosphatase is derivatized or modified, such as by biotinylation. Upon biotinylation of the alkaline phosphatase, the resulting biotinylated alkaline phosphatase is eluted or removed from attachment to the p-ABPA treated solid substrate or dextran beads to which it was fixed, yielding a biotinylated alkaline phosphatase with the active or functional site of the alkaline phosphatase intact. The complex, while still attached to the dextran beads, could be further treated with a second compound or component which might also, if desired, provide an active or functional site, such as by contact with avidin, which would bind to the modifying or derivatizing moiety, i.e. biotin, of the biotinylated alkaline phosphatase and the thus-produced avidin-biotinylated alkaline phosphatase complex then eluted. This complex, such as a complex comprising avidin-biotinylated alkaline phosphatase, could be employed as a detector in a detection system for the identification and/or location of another compound, such as a biotinylated compound, e.g. a biotinylated nucleotide or biotinylated DNA.

  制备含有一种或多种生物活性或功能性化合物的复合物的方法是：在使所述第一种化合物的活性位点受到保护的条件下，将第一种化合物（可能是一种生物活性或功能性化合物）附着或固定在固体基质上，然后在所述第一种化合物附着在所述固体基质上的同时，将所述第一种化合物与一种衍生或修饰化合物衍生或结合。然后将得到的复合物或复配的第一化合物从附着在所述固体底物上释放出来，得到复合物，所述复合物包括附着在所述第一化合物上的所述衍生化合物，所述第一化合物从固体底物上释放出来后，其功能或生物活性位点可用。例如，碱性磷酸酶最好通过配体（如碱性磷酸酶的竞争性抑制剂，如对氨基苄基膦酸（p-ABPA））附着在固体基质（如葡聚糖珠）上。首先用（p-ABPA）处理葡聚糖珠，然后让碱性磷酸酶与这样处理过的葡聚糖珠接触。竞争性抑制剂 p-ABPA 可作为配体，使碱性磷酸酶附着在葡聚糖珠上，同时占据并保护碱性磷酸酶的活性位点。然后，对这样附着的碱性磷酸酶进行衍生化或修饰，如生物素化。在对碱性磷酸酶进行生物素化后，得到的生物素化碱性磷酸酶会被洗脱或去除附着在经 p-ABPA 处理过的固体底物或葡聚糖珠上的固定物，从而得到生物素化的碱性磷酸酶，而碱性磷酸酶的活性或功能位点完好无损。当该复合物仍附着在葡聚糖珠上时，可以用第二种化合物或成分进一步处理，如果需要，这种化合物或成分还可以提供一个活性或功能位点，例如通过与阿维丁接触，与生物素化碱性磷酸酶的修饰或衍生分子（即生物素）结合，然后洗脱由此产生的阿维丁-生物素化碱性磷酸酶复合物。这种复合物，如由阿维菌素-生物素化碱性磷酸酶组成的复合物，可用作检测系统中的检测器，用于识别和/或定位另一种化合物，如生物素化化合物，如生物素化核苷酸或生物素化 DNA。
• Purification process for hybrid proteins
  申请人：Merck & Co., Inc.

  公开号：EP0244147A2

  公开（公告）日：1987-11-04

  A hybrid protein containing a β-galactosidase moiety linked to a desired protein through a renin cleavage site is adsorbed to an affinity matrix under conditions in which no or minimal nonspecific adsorption occurs. The matrix-hybrid protein complex is then washed with buffer to remove loosely bound or associated proteins and resuspended in incubation buffer and renin is added to cleave the hybrid protein from the complex. The desired protein is released to the supernatant liquid while the β-galactosidase moiety remains bound to the affinity matrix. Purification of the desired protein is accomplished by anion exchange chromatography.

  在不发生或极少发生非特异性吸附的条件下，将含有通过肾素裂解位点与所需蛋白质相连的 β-半乳糖苷酶分子的杂交蛋白吸附到亲和基质上。 然后用缓冲液洗涤基质-杂交蛋白复合物，以除去松散结合或相关的蛋白质，并将其重新悬浮在孵育缓冲液中，然后加入肾素，使杂交蛋白从复合物中裂解。 所需的蛋白质释放到上清液中，而 β-半乳糖苷酶分子仍与亲和基质结合。 所需的蛋白质通过阴离子交换色谱法纯化。
• Novel fusion protein
  申请人：AMRAD CORPORATION LIMITED

  公开号：EP0293249A1

  公开（公告）日：1988-11-30

  A recombinant DNA molecule comprising a nucleotide sequence which codes an expression for a fusion protein in which a foreign protein or peptide is fused with the enzyme glutathione-S-transferase, is disclosed, as well as expression vectors or host cells containing such a molecule. Optionally, the foreign protein or peptide is fused to the enzyme through a cleavable link. Also disclosed is an expression vector having inserted therein a nucleotide sequence capable of being expressed as the enzyme glutathione-S-transferase followed by at least one restriction endonuclease recognition site for insertion of a nucleotide sequence capable of being expressed as a foreign protein or peptide fused to the glutathione-S-transferase.

  本发明公开了一种重组 DNA 分子，该分子包含编码融合蛋白表达的核苷酸序列，在该融合蛋白中，外来蛋白或多肽与谷胱甘肽-S-转移酶融合，本发明还公开了含有这种分子的表达载体或宿主细胞。外来蛋白或多肽可通过可裂解连接与酶融合。还公开了一种表达载体，其中插入了能够作为谷胱甘肽-S-转移酶表达的核苷酸序列，其后插入了至少一个限制性内切酶识别位点，用于插入能够作为与谷胱甘肽-S-转移酶融合的外来蛋白或肽表达的核苷酸序列。
• Method of assaying substance and immunoassay element
  申请人：KONICA CORPORATION

  公开号：EP0328106A2

  公开（公告）日：1989-08-16

  Disclosed is a method of assaying a target substance in a fluid sample. In this method, a) the target substance, b) a substance which specifically binds to the target substance, to which a biological active substance which does not bind to the target substance is attached, or to which a substance which specifically binds to a biological active substance which does not bind to the target substance is attached; c) a labelled substance which is the target substance or an analogue thereof labelled with β-D-galactosidase, or which is a substance which specifically binds to the target substance, labelled with β-D-galactosidase, d) a substance which specifically binds to the biological active substance and which does not bind to the target substance, or the biological active substance, which is fixed to a carrier, which carrier exists in a porous reaction layer of an assay element, and e) a substance which specifically binds to β-D-galactosidase and which changes a signal originated from β-D-galactosidase, which is fixed to said carrier or another carrier which exists in a porous reaction layer of an assay element are reacted and the change of the signal from β-D-galactosidase is measured.

  本发明公开了一种检测流体样品中目标物质的方法。在该方法中，a) 目标物质；b) 与目标物质特异性结合的物质，其上附有不与目标物质结合的生物活性物质，或附有与不与目标物质结合的生物活性物质特异性结合的物质；c) 标记物质，该标记物质是标记有β-D-半乳糖苷酶的目标物质或其类似物，或 是标记有β-D-半乳糖苷酶的能与目标物质特异性结合的物质； d) 能与生物活性物质特异性结合但不能与目标物质或生物活性物质结合的物质，该物 质固定在载体上、e) 一种能与β-D-半乳糖苷酶特异性结合的物质，它能改变来自β-D-半乳糖苷酶的信号，该物质固定在所述载体上或存在于检测元件多孔反应层中的另一种载体上，两者发生反应，并测量来自β-D-半乳糖苷酶的信号的变化。