Provided is a microorganism that is able to efficiently produce protocatechuic acid or a salt thereof by using a saccharide as a raw material, and a method of efficiently producing protocatechuic acid or a salt thereof by using the microorganism.
Provided is a transformant having protocatechuic acid producing ability, subjected to modifications (A), (B), and (C) below:
(A) enhancement of 3-dehydroshikimate dehydratase activity;
(B) enhancement of chorismate pyruvate lyase activity; and
(C) enhancement of 4-hydroxybenzoate hydroxylase activity.
Also provided is a method of producing protocatechuic acid or a salt thereof, including the step of culturing the transformant in a reaction solution containing a saccharide so as to cause the transformant to produce protocatechuic acid or a salt thereof.
提供了一种能够以
糖类为原料高效生产
原儿茶酸或其盐的微
生物,以及一种利用该微
生物高效生产
原儿茶酸或其盐的方法。
本发明提供了一种具有
原儿茶酸生产能力的转化体,该转化体经过以下(A)、(B)和(C)改良:
(A) 提高 3-脱氢
莽草酸脱
水酶活性;
(B) 提高
氯代
丙酮酸裂解酶的活性;以及
(C) 提高
4-羟基
苯甲酸羟化酶的活性。
还提供了一种生产
原儿茶酸或其盐的方法,包括在含有糖的反应溶液中培养转化体,使转化体产生
原儿茶酸或其盐。