selina-3,7(11)-diene | 222187-60-2

• 分子结构分类: 有机化合物 - 脂质和类脂质分子 - 异戊烯醇脂质
• 英文名称: selina-3,7(11)-diene
• 英文别名: Selina-3,7(11)-dien；γ-Selinen；3,7(11)-Eudesmadiene；5,8a-dimethyl-3-propan-2-ylidene-1,2,4,4a,7,8-hexahydronaphthalene
• CAS: 222187-60-2
• 化学式: C₁₅H₂₄
• 分子量: 204.356
• InChiKey: WNRBYZQFEBIUGD-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  4.4
• 重原子数：
  15
• 可旋转键数：
  0
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.73
• 拓扑面积：
  0
• 氢给体数：
  0
• 氢受体数：
  0

反应信息

• 作为反应物：
  描述：

  大麻 、 毕澄茄烯 、 α-芹子烯 、 selina-3,7(11)-diene 、 (1S,8aR)-1-异丙基-4,7-二甲基-1,2,3,5,6,8a-六氢萘 、 α-愈创木烯 生成 germacrene C
  参考文献：
  名称：

  Sesquiterpene synthases from grand fir (abies grandis), and methods of use

  摘要：

  从大冷杉（Abies grandis）中分离和测序了编码E-α-双萜烯合酶、δ-塞林烯合酶和γ-蒿莲烯合酶的cDNA，并确定了相应的氨基酸序列。因此，提供了编码大冷杉（Abies grandis）中的E-α-双萜烯合酶（SEQ ID No：13）、δ-塞林烯合酶（SEQ ID No：20）和γ-蒿莲烯合酶（SEQ ID No：24）表达的分离DNA序列（SEQ ID No：12；SEQ ID No：19和SEQ ID No：23）。在其他方面，提供了可复制的重组克隆载体，其编码E-α-双萜烯合酶、δ-塞林烯合酶和γ-蒿莲烯合酶，或编码与E-α-双萜烯合酶、δ-塞林烯合酶或γ-蒿莲烯合酶DNA或RNA的至少一部分足够互补的碱基序列，以使其与之杂交。在其他方面，提供了经改造的宿主细胞，它们已经转化、转染、感染和/或注入了重组克隆载体和/或编码E-α-双萜烯合酶、δ-塞林烯合酶或γ-蒿莲烯合酶的DNA序列。因此，提供了用于重组表达前述重组倍半萜合酶的系统和方法，可用于促进它们的大量生产、分离和纯化。重组E-α-双萜烯合酶、δ-塞林烯合酶和γ-蒿莲烯合酶可用于在植物中获得E-α-双萜烯合酶、δ-塞林烯合酶和γ-蒿莲烯合酶的表达或增强表达，以增强倍半萜类物质的生产，或者可用于调节或表达E-α-双萜烯合酶、δ-塞林烯合酶和γ-蒿莲烯合酶，或其产物的生产。

  公开号：

  US20020038001A1

文献信息

• Gymnosperm nucleic acid molecules encoding sesquiterpene synthases and methods of use
  申请人：Washington State University Foundation

  公开号：US06265639B1

  公开（公告）日：2001-07-24

  cDNAs encoding E-&agr;-bisabolene synthase, &dgr;-selinene synthase and &ggr;-humulene synthase from Grand Fir (Abies grandis) have been isolated and sequenced, and the corresponding amino acid sequences have been determined. Accordingly, isolated DNA sequences (SEQ ID No:12 and SEQ ID No:19 and SEQ ID No:23) are provided which code for the expression of E-&agr;-bisabolene synthase (SEQ ID No:13), &dgr;-selinene synthase (SEQ ID No:20) and &ggr;-humulene synthase (SEQ ID No: 24), respectively, from Grand Fir (Abies grandis). In other aspects, replicable recombinant cloning vehicles are provided which code for E-&agr;-bisabolene synthase, &dgr;-selinene synthase and &ggr;-humulene synthase, or for a base sequence sufficiently complementary to at least a portion of E-&agr;-bisabolene synthase, &dgr;-selinene synthase or &ggr;-humulene synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding E-&agr;-bisabolene synthase, &dgr;-selinene synthase or &ggr;-humulene synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant sesquiterpene synthases that may be used to facilitate their production, isolation and purification in significant amounts. Recombinant E-&agr;-bisabolene synthase, &dgr;-selinene synthase and &ggr;-humulene synthase may be used to obtain expression or enhanced expression of E-&agr;-bisabolene synthase, &dgr;-selinene synthase and &ggr;-humulene synthase in plants, or may be otherwise employed for the regulation or expression of E-&agr;-bisabolene synthase, &dgr;-selinene synthase and &ggr;-humulene synthase.

  已经分离和测序了来自大冷杉（Abies grandis）的编码E-α-藿烯合酶、δ-塞林烯合酶和γ-葎草烯合酶的cDNA，并确定了相应的氨基酸序列。因此，提供了编码来自大冷杉的E-α-藿烯合酶（SEQ ID No：13）、δ-塞林烯合酶（SEQ ID No：20）和γ-葎草烯合酶（SEQ ID No：24）表达的隔离DNA序列（SEQ ID No：12和SEQ ID No：19和SEQ ID No：23）。在其他方面，提供了可复制的重组克隆载体，其编码E-α-藿烯合酶、δ-塞林烯合酶和γ-葎草烯合酶，或者编码足够互补至少一部分E-α-藿烯合酶、δ-塞林烯合酶或γ-葎草烯合酶DNA或RNA的碱基序列，以使其能够与其杂交。在其他方面，提供了经过改造的宿主细胞，其已转化、转染、感染和/或注入重组克隆载体和/或编码E-α-藿烯合酶、δ-塞林烯合酶或γ-葎草烯合酶的DNA序列。因此，提供了用于重组表达前述重组倍半萜合酶的系统和方法，可用于促进其大量生产、分离和纯化。重组的E-α-藿烯合酶、δ-塞林烯合酶和γ-葎草烯合酶可用于在植物中获得E-α-藿烯合酶、δ-塞林烯合酶和γ-葎草烯合酶的表达或增强表达，或者可用于调节或表达E-α-藿烯合酶、δ-塞林烯合酶和γ-葎草烯合酶。
• Sesquiterpene synthases from grand fir (Abies grandis), and methods of use
  申请人：Washington State University Research Foundation

  公开号：US06451576B1

  公开（公告）日：2002-09-17

  cDNAs encoding, E-&agr;-bisabolene synthase, &dgr;-selinene synthase and &ggr;-humulene synthase from Grand Fir (Abies grandis) have been isolated and sequenced, and the corresponding amino acid sequences have been determined. Accordingly, isolated DNA sequences (SEQ ID No:12; SEQ ID No:19 and SEQ ID No:23) are provided which code for the expression of E-&agr;-bisabolene synthase (SEQ ID No:13), &dgr;-selinene synthase (SEQ ID No:20) and &ggr;-humulene synthase (SEQ ID No:24), respectively, from Grand Fir (Abies grandis). In other aspects, replicable recombinant cloning vehicles are provided which code for E-&agr;-bisabolene synthase, &dgr;-selinene synthase and &ggr;-humulene synthase, or for a base sequence sufficiently complementary to at least a portion of E-&agr;-bisabolene synthase, &dgr;-selinene synthase or &ggr;-humulene synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding E-&agr;-bisabolene synthase, &dgr;-selinene synthase or ,&ggr;-humulene synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant sesquiterpene syntheses that may be used to facilitate their production, isolation and purification in significant amounts. Recombinant E-&agr;-bisabolene synthase, &dgr;-selinene synthase and &ggr;-humulene synthase may be used to obtain expression or enhanced expression of E-&agr;-bisabolene synthase, &dgr;-selinene synthase and &ggr;-humulene synthase in plants in order to enhance the production of sesquiterpenoids, or may be otherwise employed for the regulation or expression of E-&agr;-bisabolene synthase, &dgr;-selinene synthase and &ggr;-humulene synthase, or the production of their products.

  从大冷杉（Abies grandis）中分离和测序了编码E-α-芬烯合酶、δ-塞林烯合酶和γ-葎草烯合酶的cDNA，并确定了相应的氨基酸序列。因此，提供了编码大冷杉（Abies grandis）中的E-α-芬烯合酶（SEQ ID No:13）、δ-塞林烯合酶（SEQ ID No:20）和γ-葎草烯合酶（SEQ ID No:24）表达的分离DNA序列（SEQ ID No:12、SEQ ID No:19和SEQ ID No:23）。在其他方面，提供了可复制的重组克隆载体，其编码E-α-芬烯合酶、δ-塞林烯合酶和γ-葎草烯合酶，或编码与E-α-芬烯合酶、δ-塞林烯合酶或γ-葎草烯合酶DNA或RNA的至少一部分足够互补的碱基序列，以使其能够与之杂交。在其他方面，提供了经过改造的宿主细胞，这些细胞已被转化、转染、感染和/或注射了重组克隆载体和/或编码E-α-芬烯合酶、δ-塞林烯合酶或γ-葎草烯合酶的DNA序列。因此，提供了用于重组表达上述重组倍半萜合成物的系统和方法，可用于促进它们的大量生产、分离和纯化。重组E-α-芬烯合酶、δ-塞林烯合酶和γ-葎草烯合酶可用于在植物中获得E-α-芬烯合酶、δ-塞林烯合酶和γ-葎草烯合酶的表达或增强表达，以增强倍半萜类化合物的产生，或者可用于调节或表达E-α-芬烯合酶、δ-塞林烯合酶和γ-葎草烯合酶，或其产物的生产。
• Sesquiterpene synthases from grand fir (abies grandis), and methods of use
  申请人：Washington State University Research Foundation

  公开号：US20020038001A1

  公开（公告）日：2002-03-28

  cDNAs encoding E-&agr;-bisabolelene synthase, &dgr;-selinene synthase and &ggr;-humulene synthase from Grand Fir ( Abies grandis ) have been isolated and sequenced, and the corresponding amino acid sequences have been determined. Accordingly, isolated DNA sequences (SEQ ID No:12; SEQ ID No:19 and SEQ ID No:23) are provided which code for the expression of E-&agr;-bisabolene synthase (SEQ ID No:13), &dgr;-selinene synthase (SEQ ID No:20) and &ggr;-humulene synthase (SEQ ID No:24), respectively, from Grand Fir ( Abies grandis ). In other aspects, replicable recombinant cloning vehicles are provided which code for E-&agr;-bisabolene synthase, &dgr;-selinene synthase and &ggr;-humulene synthase, or for a base sequence sufficiently complementary to at least a portion of E-&agr;-bisabolene synthase, &dgr;-selinene synthase or &ggr;-humulene synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding E-&agr;-bisabolene synthase, &dgr;-selinene synthase or &ggr;-humulene synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant sesquiterpene synthases that may be used to facilitate their production, isolation and purification in significant amounts. Recombinant E-&agr;-bisabolene synthase, &dgr;-selinene synthase and &ggr;-humulene synthase may be used to obtain expression or enhanced expression of E-&agr;-bisabolene synthase, &dgr;-selinene synthase and &ggr;-humulene synthase in plants in order to enhance the production of sesquiterpenoids, or may be otherwise employed for the regulation or expression of E-&agr;-bisabolene synthase, &dgr;-selinene synthase and &ggr;-humulene synthase, or the production of their products.

  从大冷杉（Abies grandis）中分离和测序了编码E-α-双萜烯合酶、δ-塞林烯合酶和γ-蒿莲烯合酶的cDNA，并确定了相应的氨基酸序列。因此，提供了编码大冷杉（Abies grandis）中的E-α-双萜烯合酶（SEQ ID No：13）、δ-塞林烯合酶（SEQ ID No：20）和γ-蒿莲烯合酶（SEQ ID No：24）表达的分离DNA序列（SEQ ID No：12；SEQ ID No：19和SEQ ID No：23）。在其他方面，提供了可复制的重组克隆载体，其编码E-α-双萜烯合酶、δ-塞林烯合酶和γ-蒿莲烯合酶，或编码与E-α-双萜烯合酶、δ-塞林烯合酶或γ-蒿莲烯合酶DNA或RNA的至少一部分足够互补的碱基序列，以使其与之杂交。在其他方面，提供了经改造的宿主细胞，它们已经转化、转染、感染和/或注入了重组克隆载体和/或编码E-α-双萜烯合酶、δ-塞林烯合酶或γ-蒿莲烯合酶的DNA序列。因此，提供了用于重组表达前述重组倍半萜合酶的系统和方法，可用于促进它们的大量生产、分离和纯化。重组E-α-双萜烯合酶、δ-塞林烯合酶和γ-蒿莲烯合酶可用于在植物中获得E-α-双萜烯合酶、δ-塞林烯合酶和γ-蒿莲烯合酶的表达或增强表达，以增强倍半萜类物质的生产，或者可用于调节或表达E-α-双萜烯合酶、δ-塞林烯合酶和γ-蒿莲烯合酶，或其产物的生产。