Method for Preparing Isoprenyl Cysteine Compounds and Analogs Thereof
申请人:Rapole Keshava
公开号:US20090192332A1
公开(公告)日:2009-07-30
Methods of preparing isoprenyl cysteine compounds by coupling a cysteine compound with an activated (i.e. halogenated) lipid are disclosed. Also disclosed, among other things, are methods of making activated (i.e. halogenated) lipids, and methods of purifying isoprenyl cysteine compounds.
Processes for the stereospecific synthesis of coenzyme Q10, ubiquinone, are disclosed; a total synthetic procedure using geraniol as the starting material. The process of the invention results in high yields of isometrically pure ubiquinone. The synthetic coenzyme Q 10 can be used as an antioxidant, a nutritional supplement and as a pharmaceutical in treating many conditions.
Detection and Quantitation Method for Proteomics of Post-Translational Modifications
申请人:Sichuan University
公开号:US20200033361A1
公开(公告)日:2020-01-30
The present disclosure relates to the technical field of comparative proteomics, in particular to a detection and quantitation method for proteomics of post-translational modifications. With this method, the protein samples to be studied and internal standards are labeled with isobaric tandem mass tags, and tandem mass spectrometry analysis is carried out for the labeled peptide mixture, wherein the internal standard is a peptide mixture rich in post-translational modifications to be detected. Through this method, the signal of peptides containing the post-translational modifications to be detected can be amplified under the situation that mass spectrometer sensitivity is unchanged, and enrichment of the post-translational modification peptides is not needed. The probability of detecting the peptides containing the post-translational modifications to be detected by mass spectrometer and being selected for subsequent MS/MS analysis is increased.