phlein hexasaccharide | 137855-42-6

• 分子结构分类: 有机化合物 - 有机氧化合物
• 英文名称: phlein hexasaccharide
• 英文别名: levanopentaose；Fruf(b2-6)Fruf(b2-6)Fruf(b2-6)Fruf(b2-6)Fruf(b2-1a)Glc；(2R,3R,4S,5S,6R)-2-[(2S,3S,4S,5R)-5-[[(2R,3S,4S,5R)-5-[[(2R,3S,4S,5R)-5-[[(2R,3S,4S,5R)-5-[[(2R,3S,4S,5R)-3,4-dihydroxy-2,5-bis(hydroxymethyl)oxolan-2-yl]oxymethyl]-3,4-dihydroxy-2-(hydroxymethyl)oxolan-2-yl]oxymethyl]-3,4-dihydroxy-2-(hydroxymethyl)oxolan-2-yl]oxymethyl]-3,4-dihydroxy-2-(hydroxymethyl)oxolan-2-yl]oxymethyl]-3,4-dihydroxy-2-(hydroxymethyl)oxolan-2-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol
• CAS: 137855-42-6
• 化学式: C₃₆H₆₂O₃₁
• 分子量: 990.87
• InChiKey: JOKLMVRZIKIUMJ-TWMOFSFSSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -11.1
• 重原子数：
  67
• 可旋转键数：
  21
• 环数：
  6.0
• sp3杂化的碳原子比例：
  1.0
• 拓扑面积：
  506
• 氢给体数：
  20
• 氢受体数：
  31

反应信息

• 作为产物：
  描述：

  蔗糖 在 levansucrase from Bacillus amyloliquefaciens 、 endo-inulinase from Aspergillus niger 作用下， 以 aq. phosphate buffer 为溶剂， 生成 蔗果五糖 、 levanohexaose 、 phlein hexasaccharide 、 蔗果三糖 、 6-kestose 、 蔗果四糖
  参考文献：
  名称：

  Optimization of levansucrase/endo-inulinase bi-enzymatic system for the production of fructooligosaccharides and oligolevans from sucrose

  摘要：

  A bi-enzymatic system based on the combined use of levansucrase (LS) from Bacillus amyloliquefaciens and endo-inulinase from Aspergillus niger in a one-step reaction was investigated for the synthesis of fructooligosaccharides (FOSs) and oligolevans using sucrose as the sole substrate. Sucrose concentration was the most important independent variable, whilst LS to endo-inulinase ratio exhibited significant effects on the end-product profiles. The interaction between sucrose concentration and reaction time exhibited significant effect on all responses. At the initial stage of time course, short chain FOSs (scFOSs, 1-kestose, nystose, 1(F)-fructosylnystose) were the major products, whilst 6-kestose, medium chain fructooligosaccharides (mcFOSs, levanohexaose, levanopentaose) and oligolevans became the dominant ones at the late stage. The optimal conditions leading to a high yield of scFOSs (1:1 ratio, 0.5 h, 0.6 M) were different from those resulting in a high yield of mcFOSs and oligolevans (1.85:1 ratio, 1.77 h, 0.6 M). The bi-enzymatic system has a great potential for the production of FOSs and oligolevans at a large scale because of its high yield (57-65%, w/w) and productivity (65.8-266.8 g/L h), and its uses of low temperature (35 degrees C) and low concentration of sucrose. To the best of our knowledge, this is the first study on the optimization of a LS/endo-inulinase bi-enzymatic system. (C) 2014 Elsevier B.V. All rights reserved.

  DOI：

  10.1016/j.molcatb.2014.08.005

文献信息

• Optimization of levansucrase/endo-inulinase bi-enzymatic system for the production of fructooligosaccharides and oligolevans from sucrose
  作者：Feng Tian、Maryam Khodadadi、Salwa Karboune

  DOI：10.1016/j.molcatb.2014.08.005

  日期：2014.11

  A bi-enzymatic system based on the combined use of levansucrase (LS) from Bacillus amyloliquefaciens and endo-inulinase from Aspergillus niger in a one-step reaction was investigated for the synthesis of fructooligosaccharides (FOSs) and oligolevans using sucrose as the sole substrate. Sucrose concentration was the most important independent variable, whilst LS to endo-inulinase ratio exhibited significant effects on the end-product profiles. The interaction between sucrose concentration and reaction time exhibited significant effect on all responses. At the initial stage of time course, short chain FOSs (scFOSs, 1-kestose, nystose, 1(F)-fructosylnystose) were the major products, whilst 6-kestose, medium chain fructooligosaccharides (mcFOSs, levanohexaose, levanopentaose) and oligolevans became the dominant ones at the late stage. The optimal conditions leading to a high yield of scFOSs (1:1 ratio, 0.5 h, 0.6 M) were different from those resulting in a high yield of mcFOSs and oligolevans (1.85:1 ratio, 1.77 h, 0.6 M). The bi-enzymatic system has a great potential for the production of FOSs and oligolevans at a large scale because of its high yield (57-65%, w/w) and productivity (65.8-266.8 g/L h), and its uses of low temperature (35 degrees C) and low concentration of sucrose. To the best of our knowledge, this is the first study on the optimization of a LS/endo-inulinase bi-enzymatic system. (C) 2014 Elsevier B.V. All rights reserved.