N-glycolyl-D-mannosamine

• 分子结构分类: 有机化合物 - 有机氧化合物
• 英文名称: N-glycolyl-D-mannosamine
• 英文别名: ManNGc；Glycolyl-D-mannosamine；2-hydroxy-N-[(2S,3S,4R,5S,6R)-2,4,5-trihydroxy-6-(hydroxymethyl)oxan-3-yl]acetamide
• 化学式: C₈H₁₅NO₇
• 分子量: 237.21
• InChiKey: KSWRTSFNOKOHBE-BIAPBZAGSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -2.4
• 重原子数：
  16
• 可旋转键数：
  3
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.88
• 拓扑面积：
  140
• 氢给体数：
  6
• 氢受体数：
  7

反应信息

• 作为反应物：
  描述：

  N-glycolyl-D-mannosamine 、 丙酮酸 生成 N-glycolylneuraminic acid
  参考文献：
  名称：

  BAUMANN, WOLFGANG;FREIDENREICH, JURGEN;WEISSHAAR, GERHARD;BROSSMER, REINH+, BIOL. CHEM. /HOPPE-SEYLER, 370,(1989) N, C. 141-149

  摘要：

  DOI：
• 作为产物：
  描述：

  N-glycolylneuraminic acid 生成 N-glycolyl-D-mannosamine
  参考文献：
  名称：

  BAUMANN, WOLFGANG;FREIDENREICH, JURGEN;WEISSHAAR, GERHARD;BROSSMER, REINH+, BIOL. CHEM. /HOPPE-SEYLER, 370,(1989) N, C. 141-149

  摘要：

  DOI：

文献信息

• Human Neuraminidase Isoenzymes Show Variable Activities for 9-<i>O</i>-Acetyl-sialoside Substrates
  作者：Carmanah D. Hunter、Neha Khanna、Michele R. Richards、Reza Rezaei Darestani、Chunxia Zou、John S. Klassen、Christopher W. Cairo

  DOI：10.1021/acschembio.7b00952

  日期：2018.4.20

  Recognition of terminal sialic acids is central to many cellular processes, and structural modification of sialic acid can disrupt these interactions. A prominent, naturally occurring, modification of sialic acid is 9-O-acetylation (9-O-Ac). Study of this modification through generation and analysis of 9-O-Ac sialosides is challenging because of the lability of the acetate group. Fundamental questions

  末端唾液酸的识别是许多细胞过程的核心，唾液酸的结构修饰可以破坏这些相互作用。唾液酸的一种明显的，天然存在的修饰是9- O-乙酰化（9- O- Ac）。由于乙酸酯基团的不稳定性，通过生成和分析9- O- Ac唾液酸苷进行这种修饰的研究具有挑战性。关于9- O- Ac唾液酸的作用的基本问题仍未得到解答，包括其对人神经氨酸酶（hNEU）的识别和水解可能产生何种作用。调查9- O-乙酰化唾液酸（Neu5,9Ac 2），我们合成了乙酰化的荧光hNEU底物2'-（4-甲基伞形酮）-9 - O-乙酰基-α - d - N-乙酰基神经氨酸。此外，我们生成了一组包含改性唾液酸的辛基唾液酸乳糖苷，包括键合，9- O-乙酰化和C-5基团（Neu5Gc）的变化。使用荧光光谱法和电喷雾电离质谱法测定hNEU裂解底物的相对速率。我们报道9- O-乙酰化对hNEU的唾液酸水解具有显着的差异影响，具有一般底物耐受性，遵循Neu5Ac>
• BAUMANN, WOLFGANG;FREIDENREICH, JURGEN;WEISSHAAR, GERHARD;BROSSMER, REINH+, BIOL. CHEM. /HOPPE-SEYLER, 370,(1989) N, C. 141-149
  作者：BAUMANN, WOLFGANG、FREIDENREICH, JURGEN、WEISSHAAR, GERHARD、BROSSMER, REINH+

  DOI：——

  日期：——
• Probe Compound for Detecting and Isolating Enzymes and Means and Methods Using the Same
  申请人：Golyshin Peter N.

  公开号：US20120231972A1

  公开（公告）日：2012-09-13

  The present invention relates to a probe compound that can comprise any substrate or metabolite of an enzymatic reaction in addition to an indicator component, such as, for example, a fluorescence dye, or the like. Moreover, the present invention relates to means for detecting enzymes in form of an array, which comprises any number of probe compounds of the invention which each comprise a different metabolite of interconnected metabolites representing the central pathways in all forms of life. Moreover, the present invention relates to a method for detecting enzymes involving the application of cell extracts or the like to the array of the invention which leads to reproducible enzymatic reactions with the substrates. These specific enzymatic reactions trigger the indicator (e.g. a fluorescence signal) and bind the enzymes to the respective cognate substrates. Moreover, the invention relates to means for isolating enzymes in form of nanoparticles coated with the probe compound of the invention. The immobilisation of the cognate substrates or metabolites on the surface of nanoparticles by means of the probe compounds allows capturing and isolating the respective enzyme, e.g. for subsequent sequencing.
• [EN] PROBE COMPOUND FOR DETECTING AND ISOLATING ENZYMES AND MEANS AND METHODS USING THE SAME<br/>[FR] COMPOSÉ SONDE POUR DÉTECTER ET ISOLER DES ENZYMES ET MOYENS ET PROCÉDÉS POUR L'UTILISER
  申请人：HELMHOLTZ INFEKTIONSFORSCHUNG

  公开号：WO2010105851A1

  公开（公告）日：2010-09-23

  The present invention relates to a probe compound that can comprise any substrate or metabolite of an enzymatic reaction in addition to an indicator component, such as, for example, a fluorescence dye, or the like. Moreover, the present invention relates to means for detecting enzymes in form of an array, which comprises any number of probe compounds of the invention which each comprise a different metabolite of interconnected metabolites representing the central pathways in all forms of life. Moreover, the present invention relates to a method for detecting enzymes involving the application of cell extracts or the like to the array of the invention which leads to reproducible enzymatic reactions with the substrates. These specific enzymatic reactions trigger the indicator (e.g. a fluorescence signal) and bind the enzymes to the respective cognate substrates. Moreover, the invention relates to means for isolating enzymes in form of nanoparticles coated with the probe compound of the invention. The immobilisation of the cognate substrates or metabolites on the surface of nanoparticles by means of the probe compounds allows capturing and isolating the respective enzyme, e.g. for subsequent sequencing.