申请人:Berry David A.
公开号:US20100087634A1
公开(公告)日:2010-04-08
Fluorous-tagged oligonucleotide reagents and an oligonucleotide purification methodology making use thereof, the method comprising: Synthesizing oligonucleotides using oligonucleotide reagents each bearing at least one fluorous group to yield a mixture of synthesis products and reagents, the mixture including at least one target synthesized oligonucleotide bearing at least one fluorous group; passing the mixture through a separation medium having an affinity for the at least one fluorous group so that the target synthesized oligonucleotide bearing at least one fluorous group is adsorbed by the separation medium; washing the separation medium with at least a first solvent to selectively dissociate therefrom substantially all synthesis products and reagents of the heterogenous mixture other than the at least one target synthesized oligonucleotide bearing at least one fluorous group; and subsequently dissociating the at least one synthesized oligonucleotide from the separation medium, with or without the fluorous group.
含氟标记的寡核苷酸试剂及其利用的寡核苷酸纯化方法,该方法包括:使用每个带有至少一个氟基团的寡核苷酸试剂合成寡核苷酸,以产生一种合成产物和试剂的混合物,其中包括至少一种带有至少一个氟基团的目标合成寡核苷酸;将混合物通过具有亲和力的分离介质,使带有至少一个氟基团的目标合成寡核苷酸被分离介质吸附;使用至少一种第一溶剂清洗分离介质,以选择性地分离除带有至少一个氟基团的目标合成寡核苷酸之外的异质混合物中的所有合成产物和试剂;随后将至少一个合成寡核苷酸与或不与氟基团从分离介质中解离。