development of therapeutics. Herein, we report a strategy for the capture of protein-protein interactions using photoaffinity palladium reagents. First, the palladium-mediated reagent site specifically transferred a photoaffinity modified aryl group to the designated cysteine residue. Next, the photoaffinity group was activated by UV radiation to trap the proximal protein residue for the formation of a crosslink
蛋白质-蛋白质相互作用(P
PI)在几乎所有细胞过程中都是必不可少的。复杂P
PI的探测提供了对感兴趣的
生物系统的新见解,并为治疗剂的开发铺平了道路。在本文中,我们报告了使用光亲和性
钯试剂捕获蛋白质-蛋白质相互作用的策略。首先,
钯介导的试剂位点特异性地将光亲和力修饰的芳基转移至指定的半胱
氨酸残基。接下来,光亲和基团被紫外线辐射激活,以捕获近端蛋白质残基以形成交联。此策略用于捕获PYL-ABA-PP2C相互作用,这是
脱落酸(ABA)信号传导途径的核心。