The mode of incorporation into nicotinamide of label from13C-labeled samples of D-glucose, in Escherichia coli and Saccharomyces cerevisiae, was determined by means of13C NMR spectroscopy. The results, which confirm and extend early studies with radioactive tracers, permit a definitive choice to be made between alternative biogenetic proposals.Key words: nicotinic acid, nicotinamide, biosynthesis, glucose incorporation,13C NMR.
使用
13C标记的
D-葡萄糖样品,在大肠杆菌和酿酒酵母中,通过
13C NMR光谱法确定了尼克酰胺的合成方式。这些结果确认并扩展了早期放射性示踪剂的研究,使得可以在不同的
生物合成提议之间做出明确的选择。关键词:
烟酸,尼克酰胺,
生物合成,
葡萄糖合并,
13C NMR。