N²-n-butyl-2'-deoxyguanosine

• 分子结构分类: 有机化合物 - 核苷、核苷酸和类似物 - 嘌呤核苷
• 英文名称: N²-n-butyl-2'-deoxyguanosine
• 英文别名: N²-nBu-dG；2-(butylamino)-9-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-1H-purin-6-one
• 化学式: C₁₄H₂₁N₅O₄
• 分子量: 323.352
• InChiKey: KJHYZESUDUEXOY-IVZWLZJFSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  0.7
• 重原子数：
  23
• 可旋转键数：
  6
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.64
• 拓扑面积：
  121
• 氢给体数：
  4
• 氢受体数：
  6

反应信息

• 作为产物：
  描述：

  C₁₉H₃₃N₅O₄Si 在 溶剂黄146 作用下， 反应 24.0h， 生成 N²-n-butyl-2'-deoxyguanosine
  参考文献：
  名称：

  DNA 聚合酶 η 促进人类细胞中 N2-烷基-2'-脱氧鸟苷加合物的转录旁路

  摘要：

  为了应对未修复的 DNA 损伤，细胞配备了 DNA 损伤耐受机制，包括跨损伤合成 (TLS)。虽然 TLS 聚合酶在促进受损 DNA 模板的复制方面得到充分证明，但 TLS 聚合酶是否参与细胞中 DNA 损伤的转录旁路仍然未知。在这里，我们采用竞争性转录和加合物旁路测定来检查跨N 2 -烷基-2'-脱氧鸟苷（N 2 -烷基-dG，烷基 = 甲基、乙基、正丙基或n -丁基）HEK293T 细胞损伤。我们发现N 2-烷基-dG损伤强烈阻断转录并在新生转录物中引发CC→AA串联突变，其中腺苷与损伤及其相邻的5'核苷相对错入。此外，Pol η 的基因消融，但不是 Pol κ、Pol ι 或 Pol ζ，导致N 2 -烷基-dG 病变的转录旁路效率显着降低，这因 Pol η 缺陷中 Rev1 的共同缺失而加剧背景。我们还观察到N 2 - n Bu-dG 的修复不受 Pol η 或 Rev1 基因缺失的显着影响。因此，我们的结果提供了对N

  DOI：

  10.1021/jacs.1c07374

文献信息

• Multifunctional ligand system for cell-specific transfer of nucleic acid
  申请人：HOECHST AKTIENGESELLSCHAFT

  公开号：EP0846772A1

  公开（公告）日：1998-06-10

  The invention relates to a multifunctional ligand system that is not immunogenic and that carries out cell-specific transfer of nucleotide sequences. The system comprises at least one target cell-specific ligand, at least one linker and at least one gene construct-specific ligand, with the gene construct-specific ligand advantageously comprising an antibody, or a part thereof, which binds directly or indirectly to the gene construct. The invention also relates to ligand systems for preparing a pharmaceutical or a vaccine for the treatment or prevention of a disease of the skin, of the mucous membranes, of the nervous system, of the internal organs, of blood coagulation, of the hemopoietic system, of the immune system, of the musculature, of the sustentacular tissue or of the joints.

  本发明涉及一种多功能配体系统，该系统无免疫原性，可进行核苷酸序列的细胞特异性转移。该系统包括至少一种靶细胞特异性配体、至少一种连接体和至少一种基因构建体特异性配体，其中基因构建体特异性配体有利地包括直接或间接与基因构建体结合的抗体或其一部分。本发明还涉及配体系统，用于制备治疗或预防皮肤、粘膜、神经系统、内脏器官、血液凝固、造血系统、免疫系统、肌肉组织、软组织或关节疾病的药物或疫苗。
• Novel complex-forming proteins
  申请人：——

  公开号：US20030054409A1

  公开（公告）日：2003-03-20

  The invention relates to a complex of specifically complex-forming proteins which are not naturally occurring, comprising the following components: a) at least one ligand specific for a target structure, b) at least one protein comprising a mutated dimerization domain, the mutated dimerization domain having been derived by mutation of a naturally occurring dimerization domain, it being possible for this mutated dimerization domain to interact specifically with component c) and the component b) being connected covalently to the component a), c) at least one protein comprising a mutated dimerization domain, the mutated dimerization domain having been derived by mutation of a naturally occurring dimerization domain, it being possible for this mutated dimerization domain to interact specifically with component b) and the component c) is linked covalently to the component d), and d) at least one effector. In addition, the invention relates to the use and preparation of these complexes, and to nucleic acid constructs coding for the proteins mentioned and use thereof.

  本发明涉及一种非天然存在的特异性复合物形成蛋白的复合物，由以下组分组成：a) 至少一种针对目标结构的特异性配体； b) 至少一种包含突变二聚化结构域的蛋白质，该突变二聚化结构域是由天然存在的二聚化结构域突变而来，该突变二聚化结构域可与成分 c) 发生特异性相互作用，成分 b) 与成分 a) 共价连接、c) 至少一种包含突变二聚化结构域的蛋白质，该突变二聚化结构域是通过突变天然存在的二聚化结构域而得到的，该突变二聚化结构域可与组分 b) 发生特异性相互作用，组分 c) 与组分 d) 共价连接；以及 d) 至少一种效应物。此外，本发明还涉及这些复合物的使用和制备，以及编码所述蛋白质的核酸构建体及其使用。
• Single-chain multiple antigen-binding molecule, its preparation and use
  申请人：Kontermann Roland

  公开号：US20050004352A1

  公开（公告）日：2005-01-06

  The present invention relates to a single-chain, multiple antigen-binding molecule with diverse variable domains of a heavy and of a light chain of an immunoglobulin, which are connected in the form of a VH-VL construct, which are in turn connected together via a peptide, and to the preparation and use thereof as pharmaceutical or diagnostic aid.

  本发明涉及一种单链多抗原结合分子，该分子具有免疫球蛋白重链和轻链的多种可变结构域，这些结构域以 VH-VL 构建形式连接在一起，而这些结构域又通过肽连接在一起，本发明还涉及其制备和用作药物或诊断辅助工具。
• A Convenient Method for the Preparation of <i>N</i>²-Ethylguanine Nucleosides and Nucleotides
  作者：Magoichi Sako、Hiroyoshi Kawada、Kosaku Hirota

  DOI：10.1021/jo990500e

  日期：1999.7.1
• Single-chain Multiple Antigen-binding Molecule, Its Preparation and Use
  申请人：Kontermann Roland

  公开号：US20090326206A1

  公开（公告）日：2009-12-31

  The present invention relates to a single-chain, multiple antigen-binding molecule with diverse variable domains of a heavy and of a light chain of an immunoglobulin, which are connected in the form of a VH-VL construct, which are in turn connected together via a peptide, and to the preparation and use thereof as pharmaceutical or diagnostic aid.