N²-isobutyryl-O⁶-diphenylcarbamoyl-3'-O-azidomethyl-2'-deoxyguanosine

• 分子结构分类: 有机化合物 - 核苷、核苷酸和类似物 - 嘌呤核苷
• 英文名称: N²-isobutyryl-O⁶-diphenylcarbamoyl-3'-O-azidomethyl-2'-deoxyguanosine
• 英文别名: N2-isobutyryl-O6-diphenylcarbamoyl-3'-O-azidomethyl-2'deoxyguanosine；[9-[(2R,4S,5R)-4-(azidomethoxy)-5-(hydroxymethyl)oxolan-2-yl]-2-(2-methylpropanoylamino)purin-6-yl] N,N-diphenylcarbamate
• 化学式: C₂₈H₂₉N₉O₆
• 分子量: 587.595
• InChiKey: KVSULFYXHCSRQG-BHDDXSALSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  4.2
• 重原子数：
  43
• 可旋转键数：
  11
• 环数：
  5.0
• sp3杂化的碳原子比例：
  0.32
• 拓扑面积：
  155
• 氢给体数：
  2
• 氢受体数：
  11

反应信息

• 作为产物：
  描述：

  二苯氨基甲酰氯 在 吡啶 、 氟化铵 、 sodium azide 、 磺酰氯 、 N,N-二异丙基乙胺 作用下， 以 甲醇 、 二氯甲烷 、 乙酸乙酯 、 N,N-二甲基甲酰胺 、 环己烯 为溶剂， 反应 31.5h， 生成 N²-isobutyryl-O⁶-diphenylcarbamoyl-3'-O-azidomethyl-2'-deoxyguanosine
  参考文献：
  名称：

  Methods and compositions for incorporating nucleotides

  摘要：

  该发明提供了一种确定核苷酸序列中核酸的身份的方法和组合物，包括但不限于算法、计算机可读介质、计算机程序、设备和系统，例如使用通过合成方法测序获得的数据。该发明的方法包括纠正在核苷酸测序过程中遇到的一个或多个现象，例如使用合成方法测序。这些现象包括但不限于序列领先、序列滞后、光谱串扰以及由于照明和/或滤波器响应变化而导致的噪声。

  公开号：

  US09399799B2
• 作为试剂：
  描述：

  N²-isobutyryl-O⁶-diphenylcarbamoyl-3'-O-(methylthiomethyl)-5'-O-(tert-butyldimethylsilyl)-2'-deoxyguanosine 、 二氯甲烷 、 叠氮化钠 、 氟化铵 在 氮气 、 N,N-二甲基甲酰胺 、 二氯甲烷 、 Sodium sulfate-III 、 resultant residue 、 甲醇 、 crude product 、 ethyl acetate n-hexane 、 N²-isobutyryl-O⁶-diphenylcarbamoyl-3'-O-azidomethyl-2'-deoxyguanosine 作用下， 以 水 为溶剂， 反应 28.67h， 以to produce N2-Isobutyryl-O6-(diphenylcarbamoyl)-3′-O-azidomethyl-2′-deoxyguanosine (FIG. 10, compound 12) as a white powder (230 mg; 36% yield)的产率得到N²-isobutyryl-O⁶-diphenylcarbamoyl-3'-O-azidomethyl-2'-deoxyguanosine
  参考文献：
  名称：

  MODIFIED TEMPLATE-INDEPENDENT ENZYMES FOR POLYDEOXYNUCLEOTIDE SYNTHESIS

  摘要：

  该发明涉及一种识别聚合酶的方法，例如改性末端核苷酸转移酶（TdT），该聚合酶能够无需使用模板，将包含可移除的3'-O阻断基团的核苷酸结合到核酸启动子上。该发明还包括已识别的聚合酶以及使用该聚合酶进行预定寡核苷酸序列的新生合成的方法。

  公开号：

  US20160108382A1

文献信息

• Hydroxymethyl linkers for labeling nucleotides
  申请人：INTELLIGENT BIOSYSTEMS, INC.

  公开号：US09322050B2

  公开（公告）日：2016-04-26

  The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.

  本发明提供了用于确定核苷酸序列中核酸的身份的方法和组合物，包括但不限于算法、计算机可读介质、计算机程序、装置和系统，例如使用由合成测序方法获得的数据。本发明的方法包括纠正在核苷酸测序过程中遇到的一个或多个现象，例如使用合成测序方法。这些现象包括但不限于序列引导、序列滞后、光谱串扰以及由照明和/或滤光片响应变化引起的噪声。
• Methods And Compositions For Incorporating Nucleotides
  申请人：Gordon Steven

  公开号：US20100035253A1

  公开（公告）日：2010-02-11

  The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.

  本发明提供了用于利用通过合成方法测序获得的数据，确定核酸序列中核苷酸的身份的方法和组合物，包括但不限于算法、计算机可读介质、计算机程序、设备和系统。本发明的方法包括纠正在核苷酸测序中遇到的一个或多个现象，例如使用通过合成方法进行测序。这些现象包括但不限于序列超前、序列滞后、光谱串扰以及由于照明和/或滤光片响应变化而产生的噪声。
• Methods And Compositions For Base Calling Nucleic Acids
  申请人：GORDON STEVEN

  公开号：US20100063743A1

  公开（公告）日：2010-03-11

  The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.

  本发明提供了一种确定核酸序列中核苷酸身份的方法和组合物，包括但不限于算法、计算机可读介质、计算机程序、装置和系统，例如使用通过合成方法测序获得的数据。本发明的方法包括纠正在核苷酸测序中遇到的一个或多个现象，例如使用通过合成方法测序。这些现象包括但不限于序列提前、序列滞后、光谱串扰以及由于照明和/或滤波器响应变化而产生的噪声。
• METHODS AND COMPOSITIONS FOR SEQUENCING NUCLEIC ACIDS USING CHARGE
  申请人：Gordon Steven

  公开号：US20120052489A1

  公开（公告）日：2012-03-01

  The invention provides methods and compositions, and systems for determining the identity of nucleic acids in nucleotide sequences, including sequences with one or more homopolymer regions. The methods of the invention include improvements so as to accurately identify sequences, including the difficult homopolymer sequences that are encountered during nucleotide sequencing, such as pyrosequencing.

  本发明提供了一种用于确定核苷酸序列中核酸的身份的方法、组合物和系统，包括具有一个或多个同聚物区域的序列。本发明的方法包括改进，以准确地识别序列，包括在核苷酸测序期间遇到的难以识别的同聚物序列，例如荧光测序。
• Methods And Solutions For Inhibiting Undesired Cleaving Of Labels
  申请人：GORDON STEVEN

  公开号：US20100159531A1

  公开（公告）日：2010-06-24

  The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.

  本发明提供了方法和组合物，包括但不限于算法、计算机可读介质、计算机程序、设备和系统，用于利用从合成测序方法中获得的数据，确定核苷酸序列中核酸的身份。本发明的方法包括纠正在核苷酸测序过程中遇到的一个或多个现象，例如使用合成测序方法。这些现象包括但不限于序列领先、序列滞后、光谱串扰以及由于照明和/或滤波器响应变化而导致的噪声。