hexa-his | 64134-30-1

• 物质功能分类: 生物化工 - 多肽
• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 羧酸及其衍生物
• 英文名称: hexa-his
• 英文别名: HHHHHH；hexahistidine；(His)6；6X-histidine；H-HHHHHH-OH；6His；(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-amino-3-(1H-imidazol-5-yl)propanoyl]amino]-3-(1H-imidazol-5-yl)propanoyl]amino]-3-(1H-imidazol-5-yl)propanoyl]amino]-3-(1H-imidazol-5-yl)propanoyl]amino]-3-(1H-imidazol-5-yl)propanoyl]amino]-3-(1H-imidazol-5-yl)propanoic acid
• CAS: 64134-30-1
• 化学式: C₃₆H₄₄N₁₈O₇
• 分子量: 840.862
• InChiKey: XSYUPRQVAHJETO-WPMUBMLPSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -5.7
• 重原子数：
  61
• 可旋转键数：
  23
• 环数：
  6.0
• sp3杂化的碳原子比例：
  0.33
• 拓扑面积：
  381
• 氢给体数：
  13
• 氢受体数：
  14

制备方法与用途

生物活性

Hexa-His 是由 6 个组氨酸构成的六肽，常作为融合蛋白的金属结合位点。

多肽

六聚组氨酸是由 6 个组氨酸构成的线性多肽，序列中仅含有 His 一种氨基酸，常作为融合蛋白的金属结合位点。其主要官能团是咪唑基，另外还具有一个氨基和一个羧基。

参考质量标准

• 外观：白色粉末
• 纯度（HPLC）≥98.0%
• 醋酸根含量≤12.0%
• 水分含量≤8.0%
• 肽含量≥80.0%
• 内毒素≤50EU/mg
• 氨基酸组成分析≤±10%

反应信息

• 作为反应物：
  描述：

  hexa-his 、 麦芽七糖 在 sodium cyanoborohydride 、 溶剂黄146 作用下， 以 二甲基亚砜 为溶剂， 生成
  参考文献：
  名称：

  标记糖以在质谱分析中增强信号

  摘要：

  MALDI-MS可对大型生物分子（例如蛋白质和核酸）进行快速灵敏的分析。但是，寡糖和多糖在质谱分析中灵敏度较低，部分原因是它们具有中性和亲水性，导致电离效率低。在这项研究中，通过还原末端的适当标签修饰了醛糖，氨基醛糖，醛糖醛酸和α-酮酸这四种低聚糖，以提高其电离效率。缓激肽（BK）是一种血管活性的九肽（RPPGFSPFR），含有两个精氨酸和两个苯丙氨酸残基，被证明是麦芽七糖，GlcNAc低聚物和低聚半乳糖醛酸的出色MS信号增强剂。在以2,5-二羟基苯甲酸（2,5-DHB）为基质的MALDI-TOF-MS分析中，还原胺化制得的GalA4-BK和GalA5-BK结合物的检出限为0.1 fmol，即比未修饰的五内酯酸高约800倍。MS的显着增强归因于碱性精氨酸残基对高质子亲和力和疏水性苯基丙氨酸残基对电离的协同作用。四肽GFGR（OMe）和精氨酸连接的苯二胺（H因此2 N）2 Ph-R（OMe）

  DOI：

  10.1002/jms.1887

文献信息

• Factor VIII compositions and methods of making and using same
  申请人：Schellenberger Volker

  公开号：US10421798B2

  公开（公告）日：2019-09-24

  The present invention relates to compositions comprising factor VIII coagulation factors linked to extended recombinant polypeptide (XTEN), isolated nucleic acids encoding the compositions and vectors and host cells containing the same, and methods of making and using such compositions in treatment of factor VIII-related diseases, disorders, and conditions.

  本发明涉及由与延伸重组多肽（XTEN）连接的因子 VIII 凝血因子组成的组合物、编码该组合物的分离核酸、含有该组合物的载体和宿主细胞，以及制造和使用这种组合物治疗因子 VIII 相关疾病、失调和病症的方法。
• Coordination of Two High-Affinity Hexamer Peptides to Copper(II) and Palladium(II) Models of the Peptide−Metal Chelation Site on IMAC Resins
  作者：Ya Chen、Richard Pasquinelli、Mohammed Ataai、Richard R. Koepsel、Richard A. Kortes、Rex E. Shepherd

  DOI：10.1021/ic990520z

  日期：2000.3.1

  The coordination of peptides Ser-Pro-His-His-Gly-Gly (SPHHGG) and (His)(6) (HHHHHH) to [Pd-II(mida)(D2O)] (mida(2-) = N-methyliminodiacetate) was studied by H-1 NMR as model reactions for Cu-II(iminodiacetate)immobilized metal affinity chromatography (IMAC) sites. This is the first direct physical description of peptide coordination for IMAC. A three-site coordination is observed which involves the first, third, and fourth residues along the peptide chain. The presence of proline in position 2 of SPHHGG achieves the best molecular mechanics and bonding angles in the coordinated peptide and enhances the interaction of the serine amino nitrogen. Histidine coordination of H-1, H-3, and H-4 of (His)(6) and H-3 and H-4 of SPHHGG was detected by H-1 NMR contact shifts and H/D exchange of histidyl protons. The EPR spectra of SPHHGG and HHHHHH attached to the [Cu-II(mida)] unit were obtained for additional modeling of IMAC sites. EPR parameters of the parent [Cu(mida)(H2O)(2)] complex are representative: g(u) = 2.31; g(yy) = 2.086; g(xx) = 2.053; A(ll) = 161G; A(N) = 19G (three line, one N coupling). Increased rhombic distortion is detected relative to the starting aqua complex in the order of [Cu(mida)L] for distortion of HHHHHH > SPHHGG > (H2O)(2) The lowering of symmetry is also seen in the decrease in the N-shf coupling, presumably to the imino nitrogen of mida(2-) in the order 19 G (H2O), 16 G (SPHHGG) and 11 G (HHHHHH). Visible spectra of the [Cu(mida)(SPHHGG)] and [Cu(mida)(HHHHHH)] as a function of pH indicate coordination of one histidyl donor at ca. 4.5, two in the range of pH 5-7, and two chelate ring attachments involving the terminal amino donor for SPHHGG or another histidyl donor of HHHHHH in the pH domain of 7-8 in agreement with the [Pd-II(mida)L] derivatives which form the two-chelate-ring attachment even at lower pH as shown by the H-1 NMR methods.
• BACTERIAL HOST STRAIN EXPRESSING RECOMBINANT DSBC
  申请人：UCB Pharma, S.A.

  公开号：EP2731973A1

  公开（公告）日：2014-05-21
• FACTOR VIII COMPOSITIONS AND METHODS OF MAKING AND USING SAME
  申请人：Amunix Operating Inc.

  公开号：EP2814840A1

  公开（公告）日：2014-12-24
• US9725516B2
  申请人：——

  公开号：US9725516B2

  公开（公告）日：2017-08-08