| 1384553-98-3

• 分子结构分类: 有机化合物 - 有机氧化合物
• CAS: 1384553-98-3
• 化学式: C₄₆H₅₈N₁₈O₂₀P₂S₂
• 分子量: 1309.15
• InChiKey: JTDYXKOQLGRZDT-PIVTYYKXSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -1.59
• 重原子数：
  88.0
• 可旋转键数：
  31.0
• 环数：
  8.0
• sp3杂化的碳原子比例：
  0.43
• 拓扑面积：
  558.42
• 氢给体数：
  14.0
• 氢受体数：
  30.0

反应信息

• 作为反应物：
  描述：

  在 三乙胺 、 2-巯基乙醇 、 sodium hydroxide 作用下， 以 水 、 N,N-二甲基甲酰胺 为溶剂， 反应 1.0h， 生成
  参考文献：
  名称：

  Synthesis of photolabile transcription initiators and preparation of photocleavable functional RNA by transcription

  摘要：

  Two new photolabile adenosine-containing transcription initiators with terminal thiol and amino functionalities are chemically synthesized. Transcription in the presence of the transcription initiators under the T7 phi2.5 promoter produces 50 thiol- and amino-functionalized RNA conjugated by a photocleavable (PC) linker. Further RNA functionalization with biotin may be achieved through acyl transfer reactions from either biotinyl AMP to the RNA thiol group or biotin NHS to the RNA amino group. Photocleavage of the PC linker displays relatively fast kinetics with a half-life of 4-5 min. The availability of these transcription initiators makes new photolabile RNA accessible for affinity purification of RNA, in vitro selection of functional RNAs, and functional RNA caging. (C) 2012 Elsevier Ltd. All rights reserved.

  DOI：

  10.1016/j.bmcl.2012.05.028
• 作为产物：
  描述：

  在 三异丙基硅烷 、 三乙胺 、 三氟乙酸 作用下， 以 二氯甲烷 、 N,N-二甲基甲酰胺 为溶剂， 反应 96.75h， 生成
  参考文献：
  名称：

  Synthesis of photolabile transcription initiators and preparation of photocleavable functional RNA by transcription

  摘要：

  Two new photolabile adenosine-containing transcription initiators with terminal thiol and amino functionalities are chemically synthesized. Transcription in the presence of the transcription initiators under the T7 phi2.5 promoter produces 50 thiol- and amino-functionalized RNA conjugated by a photocleavable (PC) linker. Further RNA functionalization with biotin may be achieved through acyl transfer reactions from either biotinyl AMP to the RNA thiol group or biotin NHS to the RNA amino group. Photocleavage of the PC linker displays relatively fast kinetics with a half-life of 4-5 min. The availability of these transcription initiators makes new photolabile RNA accessible for affinity purification of RNA, in vitro selection of functional RNAs, and functional RNA caging. (C) 2012 Elsevier Ltd. All rights reserved.

  DOI：

  10.1016/j.bmcl.2012.05.028