花氰染料CY5 | 146368-15-2

• 物质功能分类: 生物 - 蛋白与多肽 - 蛋白质免疫印迹 (WB)
• 分子结构分类: 有机化合物 - 有机杂环化合物 - 吲哚及其衍生物
• 中文名称: 花氰染料CY5
• 中文别名: 荧光染料CY5
• 英文名称: Cy 5
• 英文别名: 3H-Indolium,1-[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-2-[5-[1-[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-1,3-dihydro-3,3-dimethyl-5-sulfo-2H-indol-2-ylidene]-1,3-pentadienyl]-3,3-dimethyl-5-sulfo-, inner salt；1-[6-(2,5-dioxopyrrolidin-1-yl)oxy-6-oxohexyl]-2-[(1Z,3E,5Z)-5-[1-[6-(2,5-dioxopyrrolidin-1-yl)oxy-6-oxohexyl]-3,3-dimethyl-5-sulfoindol-2-ylidene]penta-1,3-dienyl]-3,3-dimethylindol-1-ium-5-sulfonate
• CAS: 146368-15-2
• 化学式: C₄₅H₅₂N₄O₁₄S₂
• 分子量: 937.058
• InChiKey: MGIODCZGPVDROX-UHFFFAOYSA-N

物化性质

• 溶解度：
  甲醇（微溶）、水（微溶）

计算性质

• 辛醇/水分配系数(LogP)：
  3.6
• 重原子数：
  65
• 可旋转键数：
  20
• 环数：
  6.0
• sp3杂化的碳原子比例：
  0.44
• 拓扑面积：
  262
• 氢给体数：
  1
• 氢受体数：
  15

反应信息

• 作为产物：
  描述：

  N-羟基丁二酰亚胺 、 CY5,双酸 在 N,N'-二环己基碳二亚胺 作用下， 以 N,N-二甲基甲酰胺 为溶剂， 反应 10.0h， 生成 花氰染料CY5
  参考文献：
  名称：

  Novel asymmetric Cy5 dyes: Synthesis, photostabilities and high sensitivity in protein fluorescence labeling

  摘要：

  Several novel water-soluble asymmetric pentamethine cyanine dyes were synthesized. The maximum absorption and emission wavelengths of the dyes in different solvents were in the range from 647 to 665 nm and exhibited negative solvatochromism with increasing solvent polarity. The fluorescence quantum yields of the dyes were about 0.1 in water, and were obviously higher than those of hydrophobic cyanine dyes. Dyes with N-benzyl groups and N-sulfo-groups displayed greater photostability than dyes with N-carboxypentyl groups in water. The limit of detection of dye 5a for BSA was 1.2 x 10(-8) mol L-1 by high performance liquid chromatography with fluorescent director about 100-fold lower than that by UV detection (1.0 x 10(-6) mol L-1). Therefore, Dye 5a could be used to improve photostability and detection sensitivity in protein analysis. (C) 2010 Elsevier B.V. All rights reserved.

  DOI：

  10.1016/j.jphotochem.2010.01.002

文献信息

• Multivalent binding composition for nucleic acid analysis
  申请人：Element Biosciences, Inc.

  公开号：US10768173B1

  公开（公告）日：2020-09-08

  Multivalent binding compositions including a particle-nucleotide conjugate having a plurality of copies of a nucleotide attached to the particle are described. The multivalent binding compositions allow one to localize detectable signals to active regions of biochemical interaction, e.g., sites of protein-protein interaction, protein-nucleic acid interaction, nucleic acid hybridization, or enzymatic reaction, and can be used to identify sites of base incorporation in elongating nucleic acid chains during polymerase reactions and to provide improved base discrimination for sequencing and array based applications.

  多价结合组合物包括颗粒-核苷酸共轭物，颗粒上附有多个核苷酸拷贝。多价结合组合物可以将可检测信号定位到生化相互作用的活性区，如蛋白质-蛋白质相互作用、蛋白质-核酸相互作用、核酸杂交或酶反应的位点，并可用于在聚合酶反应过程中识别碱基掺入延伸核酸链的位点，以及为测序和基于阵列的应用提供更好的碱基鉴别。
• Compositions and methods for pairwise sequencing
  申请人：Element Biosciences, Inc.

  公开号：US11236388B1

  公开（公告）日：2022-02-01

  The present disclosure provides compositions and methods that employ the compositions for conducting pairwise sequencing and for generating concatemer template molecules for pairwise sequencing. The concatemers can be generated using a rolling circle amplification reaction which is conducted either on-support, or conducted in-solution and then distributed onto a support. The rolling circle amplification reaction generates concatemers containing tandem copies of a sequence of interest and at least one universal adaptor sequence. An increase in the number of tandem copies in a given concatemer increases the number of sites along the concatemer for hybridizing to multiple sequencing primers which serve as multiple initiation sites for polymerase-catalyzed sequencing reactions. When the sequencing reaction employs detectably labeled nucleotides and/or detectably labeled multivalent molecules (e.g., having nucleotide units), the signals emitted by the nucleotides or nucleotide units that participate in the parallel sequencing reactions along the concatemer yields an increased signal intensity for each concatemer.

  本公开提供了使用这些组合物进行配对测序和生成用于配对测序的连接子模板分子的组合物和方法。可以使用滚圆扩增反应生成连接体，该反应可以在支架上进行，也可以在溶液中进行，然后分布到支架上。滚圆扩增反应生成的同源物含有感兴趣序列和至少一个通用适配体序列的串联拷贝。特定连接体中串联拷贝数量的增加会增加连接体上与多个测序引物杂交的位点数量，而测序引物是聚合酶催化测序反应的多个起始位点。当测序反应使用可检测标记的核苷酸和/或可检测标记的多价分子（例如，具有核苷酸单元）时，沿连接体参与平行测序反应的核苷酸或核苷酸单元发出的信号会增加每个连接体的信号强度。
• NANOCARRIER SYSTEM FOR MICRORNAS AND USES THEREOF
  申请人：RAMOT AT TEL-AVIV UNIVERSITY LTD.

  公开号：US20160145628A1

  公开（公告）日：2016-05-26

  Described herein are novel polyglycerol-amine polymeric nanocarriers in complex with microRNAs and their uses in the treatment of cancer, in particular glioblastoma. Delivery of the polymeric nanocarriers in complex with microRNAs in cell lines and in vivo inhibited cell proliferation, cell cycle progression, cell migration and tumor growth.
• MULTIVALENT BINDING COMPOSITION FOR NUCLEIC ACID ANALYSIS
  申请人：Element Biosciences, Inc.

  公开号：US20210072234A1

  公开（公告）日：2021-03-11

  Multivalent binding compositions including a particle-nucleotide conjugate having a plurality of copies of a nucleotide attached to the particle are described. The multivalent binding compositions allow one to localize detectable signals to active regions of biochemical interaction, e.g., sites of protein-protein interaction, protein-nucleic acid interaction, nucleic acid hybridization, or enzymatic reaction, and can be used to identify sites of base incorporation in elongating nucleic acid chains during polymerase reactions and to provide improved base discrimination for sequencing and array based applications.
• METHODS FOR CELLULARLY ADDRESSABLE NUCLEIC ACID SEQUENCING
  申请人：ELEMENT BIOSCIENCES, INC.

  公开号：US20210123098A1

  公开（公告）日：2021-04-29

  Provided are methods and systems for analyzing nucleic acids in a biological sample in a manner that retains the spatial and/or cellular origin of the nucleic acids within the biological sample. Compositions and kits are also provided that enable the methods and systems of the instant disclosure.