Ethyl(2s,3s)-3-[[1-(isopentylcarbamoyl)-3-methyl-butyl]carbamoyl]oxirane-2-carboxylate | 88321-09-9

• 物质功能分类: 生物化工 - 抑制剂 - 蛋白酶体(Proteases)
• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 羧酸及其衍生物
• 英文名称: Ethyl(2s,3s)-3-[[1-(isopentylcarbamoyl)-3-methyl-butyl]carbamoyl]oxirane-2-carboxylate
• 英文别名: ethyl (2S,3S)-3-[[4-methyl-1-(3-methylbutylamino)-1-oxopentan-2-yl]carbamoyl]oxirane-2-carboxylate
• CAS: 88321-09-9
• 化学式: C₁₇H₃₀N₂O₅
• 分子量: 342.4
• InChiKey: SRVFFFJZQVENJC-TTZKSVMKSA-N

物化性质

• 熔点：
  126.2°C
• 沸点：
  477.88°C (rough estimate)
• 密度：
  1.0657 (rough estimate)
• 溶解度：
  溶于DMSO、DMF或乙醇

计算性质

• 辛醇/水分配系数(LogP)：
  2.3
• 重原子数：
  24
• 可旋转键数：
  11
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.82
• 拓扑面积：
  97
• 氢给体数：
  2
• 氢受体数：
  5

安全信息

• 危险品标志：
  Xi
• 安全说明：
  S24/25,S26,S36
• 危险类别码：
  R36/37/38
• WGK Germany：
  2
• 海关编码：
  29242990
• 危险品运输编号：
  3077
• RTECS号：
  RR0404300
• 危险性防范说明：
  P261,P305+P351+P338
• 危险性描述：
  H315,H319,H335

制备方法与用途

生物活性

Aloxistatin (E64d) 是一种不可逆的膜透过性半胱氨酸蛋白酶抑制剂，具有抑制血小板聚集的活性。研究表明，SARS-CoV-2病毒入侵依赖于半胱氨酸蛋白酶cathepsin L，而使用aloxistatin 处理可显著减少92.3%的SARS-CoV-2病毒颗粒对细胞的侵入。

靶点

Target	Value
半胱氨酸蛋白酶

体外研究

Aloxistatin 可以进入完整的血小板，并通过抑制钙蛋白酶而阻断蛋白质水解。此外，它还减弱了甲状旁腺激素诱导的细胞增殖，并抑制成骨细胞分化。

体内研究

在不同动物模型中，Aloxistatin 表现出显著的效果：

• 在仓鼠骨骼肌、心脏和肝脏组织中，100毫克/千克剂量 (p.o.) 的 Aloxistatin 强烈抑制了组织蛋白酶 B 和 L 的活性。
• 对脊椎损伤（SCI）大鼠的研究表明，在 SCI 病变区和半影区提供了神经保护作用。
• 在阿尔茨海默病动物模型中，Aloxistatin 通过抑制组织蛋白酶 B 减少了大脑淀粉样蛋白-β，并改善了记忆障碍。

用途

一种半胱氨酸蛋白酶（cysteine protease）抑制剂。它能够渗透完整的细胞并阻断钙蛋白酶的活性，从而保护蛋白质激酶 C (PKC) 不受降解。E64d 还是 cathepsin B、cathepsin H 和 cathepsin L 的抑制剂。研究发现 E64-d 可以增强自然杀伤细胞和白细胞杀菌作用，并在 Xenopus 胚胎细胞中引起有丝分裂前期和中期的细胞周期阻滞。

文献信息

• Method of Screening for Plant Defense Activators, Plant Defense Activators, and Method of Enhancing Immune Response
  申请人：Kuchitsu Kazuyuki

  公开号：US20150141252A1

  公开（公告）日：2015-05-21

  A method of screening for a plant defense activator, which enhances an immune response of a plant, from at least one candidate substance includes: contacting a plant cell in which a jasmonic acid-dependent defense pathway and a salicylic acid-dependent defense pathway of a plant defense system are capable of working independently from each other, with a candidate substance; contacting the plant cell with a trigger material that induces an immune response; and assaying the plant cell after contacting with the trigger material based on an index representing an immune response, to select a target substance that enhances an immune response of the plant. A method of enhancing an immune response of a plant includes use of a specific compound, and a plant immune response enhancer includes the specific compound.
• INHIBITION OF VASCULAR ENDOTHELIAL CELL-MEDIATED PHAGOCYTIC PROCESSES FOR TREATMENT OF DEMYELINATING CONDITIONS
  申请人：FLORIDA STATE UNIVERSITY RESEARCH FOUNDATION, INC.

  公开号：US20200165608A1

  公开（公告）日：2020-05-28

  The present invention concerns a method for treating a demyelinating condition in a subject, by administering an agent to the subject that inhibits vascular endothelial cell phagocytosis. The method of the invention is useful in treating, for example, a demyelinating condition associated with an injury, such as a spinal cord injury or traumatic brain injury, as well as other demyelinating conditions, such as multiple sclerosis.
• US9739767B2
  申请人：——

  公开号：US9739767B2

  公开（公告）日：2017-08-22
• [EN] METHOD OF SCREENING FOR PLANT DEFENSE ACTIVATORS, PLANT DEFENSE ACTIVATORS, AND METHOD OF ENHANCING IMMUNE RESPONSE<br/>[FR] PROCÉDÉ DE CRIBLAGE POUR DES ACTIVATEURS DE DÉFENSE VÉGÉTALE, ACTIVATEURS DE DÉFENSE VÉGÉTALE ET PROCÉDÉ D'AUGMENTATION DE LA RÉPONSE IMMUNITAIRE
  申请人：UNIV TOKYO SCI EDUC FOUND

  公开号：WO2012029539A1

  公开（公告）日：2012-03-08

  A method of screening for a plant defense activator, which enhances an immune response of a plant, from at least one candidate substance includes: contacting a plant cell in which a jasmonic acid-dependent defense pathway and a salicylic acid-dependent defense pathway of a plant defense system are capable of working independently from each other, with a candidate substance; contacting the plant cell with a trigger material that induces an immune response; and assaying the plant cell after contacting with the trigger material based on an index representing an immune response, to select a target substance that enhances an immune response of the plant. A method of enhancing an immune response of a plant includes use of a specific compound, and a plant immune response enhancer includes the specific compound.