赫斯特荧光染料34580 | 23555-00-2

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 苯并咪唑类
• 中文名称: 赫斯特荧光染料34580
• 中文别名: N,N-二甲基-4-(5-(4-甲基哌嗪-1-基)-1H,1'H-[2,5'-联苯并[D]咪唑]-2'-基)苯胺；赫斯特34580游离
• 英文名称: N,N-dimethyl-4-[5-(4-methyl-piperazin-1-yl)-1(3)H,1'(3')H-[2,5']bibenzoimidazolyl-2'-yl]-aniline
• 英文别名: 4-dimethylamino-1-{5'-[5''-(4'''-methylpiperazin-1'''-yl)-1H-benzimidazol-2''-yl]-3H-benzimidazol-2'-yl}benzene；Hoechst 34580；N,N-dimethyl-4-[6-[6-(4-methylpiperazin-1-yl)-1H-benzimidazol-2-yl]-1H-benzimidazol-2-yl]aniline
• CAS: 23555-00-2
• 化学式: C₂₇H₂₉N₇
• 分子量: 451.574
• InChiKey: VMCOQLKKSNQANE-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  4.4
• 重原子数：
  34
• 可旋转键数：
  4
• 环数：
  6.0
• sp3杂化的碳原子比例：
  0.26
• 拓扑面积：
  67.1
• 氢给体数：
  2
• 氢受体数：
  5

反应信息

• 作为产物：
  描述：

  3,4-二氨基苯甲腈 在 盐酸 、 sodium disulfite 、 溶剂黄146 作用下， 以 乙醇 为溶剂， 反应 13.5h， 生成 赫斯特荧光染料34580
  参考文献：
  名称：

  Kelly, David P.; Bateman, Stuart A.; Hook, Robert J., Australian Journal of Chemistry, 1994, vol. 47, # 9, p. 1751 - 1770

  摘要：

  DOI：

文献信息

• Advanced drug development and manufacturing
  申请人：Los Alamos National Security, LLC

  公开号：EP2511844A2

  公开（公告）日：2012-10-17

  There is described an apparatus for measuring protein characteristics comprising an X-ray fluorescence (XRF) spectrometer comprising a source of polychromatic X-rays, an X-ray detector, a protein, a molecule that has been exposed to and at least weakly binds to the protein, a plurality of X-ray fluorescence signal data obtained by irradiating chemical elements in the protein and molecule with the polychromatic X-rays and a security system for maintaining records for the data from the plurality of X-ray fluorescence signal measurements. There is also described an x-ray microscope for measuring a sample.

  描述了一种测量蛋白质特性的仪器，该仪器包括一个 X 射线荧光 (XRF) 光谱仪，其中包括一个多色 X 射线源、一个 X 射线探测器、一个蛋白质、一个已暴露于该蛋白质并至少与该蛋白质弱结合的分子、通过用多色 X 射线照射蛋白质和分子中的化学元素而获得的多个 X 射线荧光信号数据，以及一个用于维护多个 X 射线荧光信号测量数据记录的安全系统。此外，还介绍了一种用于测量样品的 X 射线显微镜。
• METHOD AND KIT FOR DETERMINING THE AMOUNT OF DNA IN SAMPLES, AND USE THEREOF
  申请人：Univerzita Palackého v Olomouci

  公开号：EP3395956A1

  公开（公告）日：2018-10-31

  The present invention concerns a method of determining the amount of DNA in samples, comprising the following steps: (i) a sample is incubated in a solution of at least one fluorescent substance selected from the group consisted of 4',6-diamidino-2-phenylindole, 2-(4-hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bi-1H-benzimidazole, 2'-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bi-1H-benzimidazole and N,N-dimethyl-4-[5-(4-methyl-1-piperazinyl)[2,5'-bi-1H-benzimidazole]-2'-yl]benzenamine, which, in the course of this incubation, binds to the DNA present in the sample; (ii) the sample is washed with a washing solution and/or water, which washed away the fluorescent substances not bound to the DNA in the sample; (iii) the fluorescent substances bound to the DNA in the sample are eluted from the DNA with an aqueous elution solution of at least one substance selected from the group consisted of sodium dodecylsulphate, lithium dodecylsulphate and sodium [dodecanoyl(methyl)amino] acetate, wherein the concentration of sodium dodecylsulphate, lithium dodecylsulphate and/or sodium [dodecanoyl(methyl)amino]acetate is at least 0.1 % (w/v); (iv) the amount of DNA is determined in the elution solution from the previous step by measuring the fluorescent signal of the fluorescent substance used in step (i). The present invention further relates to a method of determining the amount of 5-ethynyl-2'-deoxyuridine and/or 5-bromo-2'-deoxyuridine and/or 5-chloro-2'-deoxyuridine and/or 5-iodo-2'-deoxyuridine incorporated into DNA and/or the content of proteins and/or the amount of RNA and/or the amount of specific DNA sequences and/or the amount of sugars and/or the amount of lipids and/or the amount of fluorescently-labelled DNA and/or fluorescently-labelled RNA and/or fluorescently-labelled proteins in cells and tissues; to a method of determining the replication activity in cells and tissues; to a kit for determining the amount of DNA in a sample, and to the use thereof.

  本发明涉及一种测定样品中 DNA 含量的方法，包括以下步骤： (i) 将样品置于至少一种荧光物质的溶液中孵育，该荧光物质选自以下组别：4',6-二脒基-2-苯基吲哚、2-(4-羟基苯基)-5-(4-甲基-1-哌嗪基)-2,5'-双-1H-苯并咪唑、2'-(4-乙氧基苯基)-5-(4-甲基-1-哌嗪基)-2,5'-bi-1H-苯并咪唑和 N,N-二甲基-4-[5-(4-甲基-1-哌嗪基)[2,5'-bi-1H-苯并咪唑]-2'-基]苯胺，在孵育过程中与样品中的 DNA 结合； (ii) 用洗涤液和/或水清洗样品，洗去样品中未与 DNA 结合的荧光物质； (iii) 用至少一种选自十二烷基硫酸钠、十二烷基硫酸锂和[十二烷酰基(甲基)氨基]乙酸钠组的物质的洗脱液洗脱样品中与 DNA 结合的荧光物质，其中十二烷基硫酸钠、十二烷基硫酸锂和/或[十二烷酰基(甲基)氨基]乙酸钠的浓度至少为 0.1 % (w/v)； (iv) 通过测量步骤(i)中使用的荧光物质的荧光信号，确定上一步洗脱液中 DNA 的量。 本发明还涉及一种测定 5-乙炔基-2'-脱氧尿苷和/或 5-溴-2'-脱氧尿苷和/或 5-氯-2'-脱氧尿苷和/或 5-碘-2'-脱氧尿苷的量的方法。或细胞和组织中荧光标记 DNA 和/或荧光标记 RNA 和/或荧光标记蛋白质的量；确定细胞和组织中复制活性的方法；确定样品中 DNA 含量的试剂盒及其用途。
• Method and process for quantitative evaluation of pharmacokinetics, therapeutic effects and safety of delivery of drug compounds to the inner ear for treatment of auditory disease
  申请人：The Charles Stark Draper Laboratory, Inc.

  公开号：US10596267B2

  公开（公告）日：2020-03-24

  The present disclosure provides compositions and methods for treating an auditory disease in a subject in need thereof comprising administering an effective amount of a gel-based precursor that includes an inner ear-specific therapeutic compound directly into the cochlea of the subject.

  本公开提供了用于治疗有需要的受试者听觉疾病的组合物和方法，包括将有效量的凝胶前体直接注入受试者的耳蜗，该凝胶前体包括内耳特异性治疗化合物。
• Counterstains for a biological sample
  申请人：Key Marc

  公开号：US10718694B2

  公开（公告）日：2020-07-21

  The present disclosure relates to counterstains for staining a biological sample on a single slide in preparation for microscopic examination. The counterstains are used to analyze the sample on the single slide using both brightfield and fluorescent illumination. The counterstains can identify both morphological details and molecular structures within the cells contained in the sample. The counterstains can be used in conjunction with other molecular stains.

  本公开涉及用于在单张载玻片上对生物样本进行染色以备显微镜检查的反染色剂。使用明视野和荧光照明对单张载玻片上的样本进行分析。反印迹可以识别样本中细胞的形态细节和分子结构。反印迹可与其他分子染色法结合使用。
• Methods to mobilize tissue resident cells for adoptive t cell therapy
  申请人：Emory University

  公开号：US11213546B2

  公开（公告）日：2022-01-04

  The present disclosure includes compositions, methods, and uses for a subset of T cells, SP T (TSP) cells, which display a quiescent (G0) phenotype. Aspects of the disclosure include methods for obtaining and mobilizing TSP cells in a subject. Other aspects include methods of adoptive cell transfer in a subject utilizing TSP cells.

  本公开包括显示静止（G0）表型的 T 细胞亚群 SP T（TSP）细胞的组合物、方法和用途。本公开的各个方面包括在受试者体内获得和动员 TSP 细胞的方法。其他方面包括利用 TSP 细胞在受试者体内进行收养性细胞转移的方法。