isopropyl-β-D-thiogalactopyranoside

• 分子结构分类: 有机化合物 - 有机氧化合物
• 英文名称: isopropyl-β-D-thiogalactopyranoside
• 英文别名: (2R,3R,4S,5R,6R)-2-(hydroxymethyl)-6-propan-2-ylsulfanyloxane-3,4,5-triol
• 化学式: C₉H₁₈O₅S
• 分子量: 238.305
• InChiKey: BPHPUYQFMNQIOC-QMGXLNLGSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -0.5
• 重原子数：
  15
• 可旋转键数：
  3
• 环数：
  1.0
• sp3杂化的碳原子比例：
  1.0
• 拓扑面积：
  115
• 氢给体数：
  4
• 氢受体数：
  6

反应信息

• 作为反应物：
  描述：

  isopropyl-β-D-thiogalactopyranoside 、 3,5-二氟苯磺酰氯 在 二丁基二氯化锡 、 1,2,2,6,6-五甲基哌啶 作用下， 以 丙酮 为溶剂， 反应 12.17h， 以94%的产率得到isopropyl 3-O-(3,5-difluorobenzensulfonyl)-α-D-thiogalactopyranoside
  参考文献：
  名称：

  Chemo- and Regioselective Monosulfonylation of Nonprotected Carbohydrates Catalyzed by Organotin Dichloride under Mild Conditions

  摘要：

  The catalytic regioselective monosulfonylation of nonprotected carbohydrates using organotin dichloride under mild conditions is examined. The carbohydrates were chemo- and regioselectively converted to the corresponding monosulfonates in the presence of monoalcohols using catalytic dibutyltin dichloride. The regioselectivity of the sulfonylation is attributed to the intrinsic character of the carbohydrates derived from the relative stereochemistry between their hydroxy groups.

  DOI：

  10.1021/jo301353k
• 作为试剂：
  描述：

  glucose 在 5-氨基乙酰丙酸 、 isopropyl-β-D-thiogalactopyranoside 作用下， 反应 48.0h， 生成 indigo
  参考文献：
  名称：

  Biosynthesis of indigo in Escherichia coli expressing self-sufficient CYP102A from Streptomyces cattleya

  摘要：

  Cytochrome P450 monooxygenases (CYP) are a superfamily of heme-thiolate proteins which catalyze the incorporation of oxygen atoms into substrates. Here, a self-sufficient CYP102A from Streptomyces cattleya (CYP102A_scat) was cloned, produced recombinantly in Escherichia coli strain BL21 (DE3), and the characteristic features were investigated. However, unlike other self-sufficient CYP102A enzymes that have been reported, CYP102A_scat was found to be able to catalyze intracellular hydroxylation of indole molecules with 3-C specific regioselectivity. Consequently, E. coli strains producing CYP102A_scat could synthesize approximately 1.0 g/L of indigo in LB media. Optimization of indigo synthesis was investigated through additional feeding of indole precursors such as glucose, L-tryptophan, and indole. Indigo production reached up to 3.8 +/- 0.1 g/L by adding 20 mu M of extracellular indole and 0.2 mM of L-tryptophan to the LB media. To our knowledge, this is a record and the highest yield achieved so far. 2017 Elsevier Ltd. All rights reserved.

  DOI：

  10.1016/j.dyepig.2017.01.029

文献信息

• Glycoconjugate and sugar nucleotide synthesis using solid supports
  申请人：——

  公开号：US20020150968A1

  公开（公告）日：2002-10-17

  This invention relates to methods and compositions for the in vitro production of glycoconjugates. In particular, a preferred production system is provided that comprises a solid support, at least one sugar nucleotide producing enzyme, at least one glycosyltransferase, at least one bioenergetic, and at least one acceptor. The sugar nucleotide producing enzyme(s) is preferably immobilized on the solid support. The glycosyltransferase may be co-immobilized on the solid support with the sugar nucleotide producing enzyme(s), or may be provided to the solid support in solution.

  本发明涉及用于体外制备糖基共轭物的方法和组合物。特别地，提供了一种首选的生产系统，该系统包括固体支持物、至少一种糖核苷酸产生酶、至少一种糖基转移酶、至少一种生物能量和至少一种受体。最好将糖核苷酸产生酶固定在固体支持物上。糖基转移酶可以与糖核苷酸产生酶共同固定在固体支持物上，或者可以以溶液形式提供给固体支持物。
• Live vaccine and its method of production
  申请人：Research Corporation

  公开号：EP0080806A1

  公开（公告）日：1983-06-08

  A vaccine for the immunization of a vertebrate comprising a non-pathogenic microbe that expresses a recombinant gene derived from an organism that is a pathogen of, or that produces an allergen of, said animal, where the gene codes for a gene product that is an antigen capable of being recognized by the immune system of the vertebrate and inducing antibody formation against the pathogen or allergen. ' The use of microbes of the nature described above to produce non-living vaccines comprising such gene products essentially in the absence of other antigenic material from the parent organism.

  一种用于脊椎动物免疫接种的疫苗，包括一种非致病性微生物，该微生物表达一种重组基因，该基因来源于一种生物体，该生物体是所述动物的病原体，或产生所述动物的过敏原，其中该基因编码的基因产物是一种抗原，能够被脊椎动物的免疫系统识别，并诱导形成针对病原体或过敏原的抗体。 '使用上述性质的微生物生产非活体疫苗，该疫苗包含这种基因产物，基本上不需要来自母体生物体的其他抗原物质。
• The cloning and expression of nucleotide sequences encoding bovine growth hormone
  申请人：MGI PHARMA, INC.

  公开号：EP0111814A2

  公开（公告）日：1984-06-27

  Methods and compositions are provided for the cloning and expression of bovine growth hormone (BGH) genetic sequences in single-cell host organisms. Also described are methods for culturing these novel single cell organisms to produce polypeptides having growth hormone activity and methods for purification of the gene product. Recombinant plasmids were constructed so as to provide an "optimal" system for the expression of BGH gene sequences in compatible host cells. The BGH related proteins produced by the recombinant DNA techniques described herein may be used in animal husbandry and in veterinary medicine.

  本文提供了在单细胞宿主生物体中克隆和表达牛生长激素（BGH）基因序列的方法和组合物，还描述了培养这些新型单细胞生物体以产生具有生长激素活性的多肽的方法以及纯化基因产物的方法。 重组质粒的构建为在兼容的宿主细胞中表达 BGH 基因序列提供了一个 "最佳 "系统。 本文描述的 DNA 重组技术产生的 BGH 相关蛋白可用于畜牧业和兽医学。
• Immunologically reactive non-glycosylated amino acid chains of glycoprotein B of herpes virus types 1 and 2
  申请人：Person, Stanley, Dr.

  公开号：EP0133063A1

  公开（公告）日：1985-02-13

  Plasmids are constructed containing the nucleotide sequence for selectively expressing immunologically active amino acid chains of glycoprotein B of HSV-1 and HSV-2 and chains are prepared to serve in vaccines.

  构建的质粒含有选择性表达 HSV-1 和 HSV-2 糖蛋白 B 的免疫活性氨基酸链的核苷酸序列，并制备了用于疫苗的氨基酸链。
• A xenogeneic expression control system, a method of using it, expression vectors containing it, cells transformed thereby and heterologous proteins produced therefrom
  申请人：GENENTECH, INC.

  公开号：EP0136907A2

  公开（公告）日：1985-04-10

  A portable supplementary control system comprising a promoter/operator operably linked to a gene encoding the desired heterologous protein plus a gene encoding a compatible supplementary repressor protein operably linked to an inducible promotor, thus remedying any deficiency of repressor provided by the host's own genome, so that expression of a gene encoding a heterologous protein is repressed until a favourable point in the growth cycle of the host. The control system offers a method whereby heterologous protein production can be regulated by manipulation of the environment or of the medium.

  一种便携式补充控制系统，包括一个与编码所需异源蛋白的基因可操作连接的启动子/操作器，以及一个与可诱导启动子可操作连接的编码兼容补充抑制蛋白的基因，从而弥补宿主自身基因组所提供的抑制因子的不足，使编码异源蛋白的基因的表达受到抑制，直到宿主生长周期中的有利时刻。该控制系统提供了一种方法，可以通过对环境或培养基的操作来调节异源蛋白的生产。