Phosphoric acid diethyl ester 2-formyl-4-nitro-phenyl ester | 150196-45-5

• 分子结构分类: 有机化合物 - 苯类化合物 - 苯和取代衍生物
• 英文名称: Phosphoric acid diethyl ester 2-formyl-4-nitro-phenyl ester
• 英文别名: Diethyl 2-formyl-4-nitrophenyl phosphate；diethyl (2-formyl-4-nitrophenyl) phosphate
• CAS: 150196-45-5
• 化学式: C₁₁H₁₄NO₇P
• 分子量: 303.208
• InChiKey: IZQXLPPZRBWITE-UHFFFAOYSA-N

物化性质

• 沸点：
  394.8±42.0 °C(Predicted)
• 密度：
  1.357±0.06 g/cm3(Predicted)

计算性质

• 辛醇/水分配系数(LogP)：
  1.4
• 重原子数：
  20
• 可旋转键数：
  7
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.36
• 拓扑面积：
  108
• 氢给体数：
  0
• 氢受体数：
  7

反应信息

• 作为反应物：
  描述：

  Phosphoric acid diethyl ester 2-formyl-4-nitro-phenyl ester 在 palladium on activated charcoal 三甲基溴硅烷 、 二乙胺基三氟化硫 、 氢气 、 1-(3-二甲基氨基丙基)-3-乙基碳二亚胺 作用下， 以 二氯甲烷 为溶剂， 反应 0.42h， 生成
  参考文献：
  名称：

  Direct Screening for Phosphatase Activity by Turnover-Based Capture of Protein Catalysts This work was supported by BBSRC studentships (to J.B. and H.D.), by an EPSRC studentship (to J.H.R.), and by an EC TMR studentship (to S.C.-T.).

  摘要：

  DOI：

  10.1002/1521-3773(20020301)41:5<775::aid-anie775>3.0.co;2-f
• 作为产物：
  描述：

  氯磷酸二乙酯 、 5-硝基水杨醛 在 三乙胺 作用下， 以 二氯甲烷 为溶剂， 反应 1.0h， 以85%的产率得到Phosphoric acid diethyl ester 2-formyl-4-nitro-phenyl ester
  参考文献：
  名称：

  [EN] QUINONE METHIDE ANALOG SIGNAL AMPLIFICATION
  [FR] AMPLIFICATION DU SIGNAL D'ANALOGUES DE QUINONEMÉTHIDES

  摘要：

  本文披露了一种新型的醌甲烯类似物前体，以及使用该前体的方法和套件的实施例，用于检测生物样本中的一个或多个靶标。检测方法包括将样本与检测探针接触，然后将样本与包含酶的标记共轭物接触。酶与包含可检测标记的醌甲烯类似物前体相互作用，形成具有可检测标记的反应性醌甲烯类似物，该类似物与生物样本中的靶标近距离或直接结合。然后检测可检测标记。在某些实施例中，通过多个醌甲烯类似物前体与不同酶相互作用，无需酶去活化步骤即可检测多个靶标。

  公开号：

  WO2015124703A1

文献信息

• Activity-based fluorescent probes that target phosphatases
  作者：Qing Zhu、Xuan Huang、Grace Y.J. Chen、Shao Q. Yao

  DOI：10.1016/s0040-4039(03)00348-4

  日期：2003.3

  We have successfully designed and synthesized two fluorescently-labeled, activity-based probes, Probe I and Probe 2, which were shown to label protein tyrosine phosphatases specifically, as well as other types of phosphatases. The probes were not reactive towards the other non-phosphatase enzymes tested. These probes may find potential applications in large-scale proteomic experiments whereby subclasses of proteins may be selectively identified. (C) 2003 Elsevier Science Ltd. All rights reserved.
• QUINONE METHIDE ANALOG SIGNAL AMPLIFICATION
  申请人：Ventana Medical Systems, Inc.

  公开号：US20170089911A1

  公开（公告）日：2017-03-30

  Disclosed herein are novel quinone methide analog precursors and embodiments of a method and a kit of using the same for detecting one or more targets in a biological sample. The method of detection comprises contacting the sample with a detection probe, then contacting the sample with a labeling conjugate that comprises an enzyme. The enzyme interacts with a quinone methide analog precursor comprising a detectable label, forming a reactive quinone methide analog, which binds to the biological sample proximally to or directly on the target. The detectable label is then detected. In some embodiments, multiple targets can be detected by multiple quinone methide analog precursors interacting with different enzymes without the need for an enzyme deactivation step.
• [EN] QUINONE METHIDE ANALOG SIGNAL AMPLIFICATION<br/>[FR] AMPLIFICATION DU SIGNAL D'ANALOGUES DE QUINONEMÉTHIDES
  申请人：VENTANA MED SYST INC

  公开号：WO2015124703A1

  公开（公告）日：2015-08-27

  Disclosed herein are novel quinone methide analog precursors and embodiments of a method and a kit of using the same for detecting one or more targets in a biological sample. The method of detection comprises contacting the sample with a detection probe, then contacting the sample with a labeling conjugate that comprises an enzyme. The enzyme interacts with a quinone methide analog precursor comprising a detectable label, forming a reactive quinone methide analog, which binds to the biological sample proximally to or directly on the target. The detectable label is then detected. In some embodiments, multiple targets can be detected by multiple quinone methide analog precursors interacting with different enzymes without the need for an enzyme deactivation step.

  本文披露了一种新型的醌甲烯类似物前体，以及使用该前体的方法和套件的实施例，用于检测生物样本中的一个或多个靶标。检测方法包括将样本与检测探针接触，然后将样本与包含酶的标记共轭物接触。酶与包含可检测标记的醌甲烯类似物前体相互作用，形成具有可检测标记的反应性醌甲烯类似物，该类似物与生物样本中的靶标近距离或直接结合。然后检测可检测标记。在某些实施例中，通过多个醌甲烯类似物前体与不同酶相互作用，无需酶去活化步骤即可检测多个靶标。
• Direct Screening for Phosphatase Activity by Turnover-Based Capture of Protein Catalysts This work was supported by BBSRC studentships (to J.B. and H.D.), by an EPSRC studentship (to J.H.R.), and by an EC TMR studentship (to S.C.-T.).
  作者：Jason R. Betley、Sandro Cesaro-Tadic、Abdelaziz Mekhalfia、James H. Rickard、Hazel Denham、Lynda J. Partridge、Andreas Plückthun、G. Michael Blackburn

  DOI：10.1002/1521-3773(20020301)41:5<775::aid-anie775>3.0.co;2-f

  日期：2002.3.1