1(3h)-Isobenzofuranone, 3-[4-hydroxy-2-methyl-5-(1-methylethyl)phenyl]-3-[2-methyl-5-(1-methylethyl)-4-(phosphonooxy)phenyl]- | 17016-43-2

• 分子结构分类: 有机化合物 - 脂质和类脂质分子 - 异戊烯醇脂质
• 英文名称: 1(3h)-Isobenzofuranone, 3-[4-hydroxy-2-methyl-5-(1-methylethyl)phenyl]-3-[2-methyl-5-(1-methylethyl)-4-(phosphonooxy)phenyl]-
• 英文别名: [4-[1-(4-hydroxy-2-methyl-5-propan-2-ylphenyl)-3-oxo-2-benzofuran-1-yl]-5-methyl-2-propan-2-ylphenyl] dihydrogen phosphate
• CAS: 17016-43-2
• 化学式: C₂₈H₃₁O₇P
• 分子量: 510.5
• InChiKey: JWBPMLSZYOGYFD-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  5.5
• 重原子数：
  36
• 可旋转键数：
  6
• 环数：
  4.0
• sp3杂化的碳原子比例：
  0.32
• 拓扑面积：
  113
• 氢给体数：
  3
• 氢受体数：
  7

文献信息

• Novel phosphoric ester and a method for quantitative analysis of an alkaline phosphatase using the same as a substrate
  申请人：Fuji Photo Film Co., Ltd.

  公开号：EP0061731A2

  公开（公告）日：1982-10-06

  New phosphoric ester having the following formula (I) and a method for the analysis of an alkaline phosphatase, using the phosphoric ester as a substrate; wherein M is a hydrogen atom or an alkali metal atom; A is phenyl, naphthyl, 2- or 4-pyridyl, 2- or 4-pyrimidyl, pyrazinyl, sym-triazinyl, 2-imidazolyl, 2-oxazolyl, 2-thiazolyl, or sym-thiadiazolyl group which may or may not be substituted with other substituent(s) or may form a condensed ring together with a benzene ring, if possible; B is paraphenylene group or may form naphthylene group by condensation together with a benzene ring, said groups may or may not be substituted with other substituent(s).

  具有下式(I)的新磷酸酯和以该磷酸酯为底物分析碱性磷酸酶的方法； 其中 M 是氢原子或碱金属原子；A 是苯基、萘基、2-或 4-吡啶基、2-或 4-嘧啶基、吡嗪基、共三嗪基、2-咪唑基、2-恶唑基、2-噻唑基或共噻二唑基，这些基团可以或不可以被其他取代基取代，或者可以与苯环一起形成缩合环；B 是对苯二甲酰基，或可与苯环缩合形成萘基，上述基团可被或不被其他取代基取代。
• Dry analytical element for alkaline phosphatase assay
  申请人：EASTMAN KODAK COMPANY (a New Jersey corporation)

  公开号：EP0132830A2

  公开（公告）日：1985-02-13

  Disclosed herein is a dry analytical element and a method of using same for quantitatively detecting alkaline phosphatase in an aqueous liquid. The element comprises, in fluid contact, first and second reagent zones wherein the first reagent zone contains a substrate for alkaline phosphatase, e.g. p-nitrophenyl phosphate, and the second reagent zone contains a buffer which is an alkali metal or ammonium salt and has a pKa in the range of 9-11.5, e.g. an alkali metal salt of carbonic acid.

  本文公开了一种干式分析元件及其使用方法，用于定量检测水性液体中的碱性磷酸酶。该元件包括流体接触的第一试剂区和第二试剂区，其中第一试剂区含有碱性磷酸酶的底物，例如对硝基苯磷酸，第二试剂区含有缓冲液，缓冲液是碱金属盐或铵盐，pKa 在 9-11.5 之间，例如碳酸的碱金属盐。
• Integral multilayer analytical element for use in the measurement of alkaline phosphatase acitivity
  申请人：FUJI PHOTO FILM CO., LTD.

  公开号：EP0182179A1

  公开（公告）日：1986-05-28

  An integral multilayer analytical element for use in the measurement of alkaline phosphatase activity, which comprises a porous spreading layer of woven fabric or knitted fabric containing an alkaline phosphatase-sensitive self-developing substrate, a buffer layer containing one or more compounds functioning as a phosphoric acid acceptor and a buffering agent and a support layer in a laminated form.

  一种用于测量碱性磷酸酶活性的整体式多层分析元件，它包括多孔的机织物或针织物铺展层，其中含有对碱性磷酸酶敏感的自显影基质；缓冲层，其中含有一种或多种用作磷酸受体和缓冲剂的化合物；以及层压形式的支撑层。
• Integral multilayer analytical element for measurement of alkaline phosphatase activity
  申请人：FUJI PHOTO FILM CO., LTD.

  公开号：EP0205950A1

  公开（公告）日：1986-12-30

  An integral multilayer analytical element for the measurement of alkaline phosphatase activity, which comprises a porous spreading layer containing a self-developable substrate in response to contact with alkaline phosphatase, a buffer layer containing a diffusion-resistant base and a support in a laminated form.

  一种用于测量碱性磷酸酶活性的整体多层分析元件，它包括一个多孔铺展层，内含与碱性磷酸酶接触后可自动显影的基底；一个缓冲层，内含抗扩散基底；以及一个层叠形式的支撑物。
• Hydrolyzable fluorescent substrates for phosphatases and analytical use thereof
  申请人：EASTMAN KODAK COMPANY (a New Jersey corporation)

  公开号：EP0232129A2

  公开（公告）日：1987-08-12

  Hydrolyzable substrates for acid and alkaline phosphatases comprise blocked dye moieties which, when cleaved from the substrate during hydrolysis, provide fluorescent dyes having maximum absorptions above 530 nm and maximum fluorescent emissions at least 580 nm. The dyes are blocked prior to hydrolysis with a phosphono or thioxophosphono group or a salt thereof. These substrates can be used in analytical determinations of acid or alkaline phosphatase, or in competitive binding reactions to determine immunologically reactive substances.

  酸性和碱性磷酸酶的可水解底物包括被封端的染料分子，当水解过程中染料分子从底物上裂解时，可产生最大吸收波长高于 530 nm、最大荧光发射波长至少为 580 nm 的荧光染料。 这些染料在水解前被膦酰基或硫代膦酰基或其盐封端。 这些底物可用于酸性或碱性磷酸酶的分析测定，或用于竞争性结合反应以测定免疫反应性物质。