alpha-D-苏-己糖吡喃糖苷-(1->4)-alpha-D-苏-己糖吡喃糖苷-(1->4)-alpha-D-苏-己糖吡喃糖苷-(1->6)-beta-D-苏-吡喃己糖 | 13264-95-4

• 分子结构分类: 有机化合物 - 有机氧化合物
• 中文名称: alpha-D-苏-己糖吡喃糖苷-(1->4)-alpha-D-苏-己糖吡喃糖苷-(1->4)-alpha-D-苏-己糖吡喃糖苷-(1->6)-beta-D-苏-吡喃己糖
• 英文名称: 4²-α-isomaltosylmaltose
• 英文别名: alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-alpha-D-Glcp；(2S,3R,4S,5S,6R)-2-[[(2R,3S,4S,5R,6R)-6-[(2R,3S,4R,5R,6R)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2R,3S,4R,5R,6S)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-3,4,5-trihydroxyoxan-2-yl]methoxy]-6-(hydroxymethyl)oxane-3,4,5-triol
• CAS: 13264-95-4
• 化学式: C₂₄H₄₂O₂₁
• 分子量: 666.585
• InChiKey: FPBCRLIOSBQLHS-UJDJLXLFSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -9
• 重原子数：
  45
• 可旋转键数：
  10
• 环数：
  4.0
• sp3杂化的碳原子比例：
  1.0
• 拓扑面积：
  348
• 氢给体数：
  14
• 氢受体数：
  21

反应信息

• 作为反应物：
  描述：

  alpha-D-苏-己糖吡喃糖苷-(1->4)-alpha-D-苏-己糖吡喃糖苷-(1->4)-alpha-D-苏-己糖吡喃糖苷-(1->6)-beta-D-苏-吡喃己糖 在 4-α-isomaltooligosylglucose 4-glucanohydrolase from Sarocladium kiliense U4520 作用下， 以 aq. phosphate buffer 为溶剂， 反应 0.33h， 生成 可溶性淀粉 、 α-isomaltose
  参考文献：
  名称：

  发现一种新型葡聚糖水解酶 4-α-异麦芽寡糖基葡萄糖 4-葡聚糖水解酶，可用于高效生产异麦芽糖

  摘要：

  我们在寻求改进异麦芽糖生产方法的过程中发现了一种新型酶。来自Sarocladium kiliense U4520的 4-α-异麦芽寡糖基葡萄糖 4-葡聚糖水解酶可识别泛糖基序 (α- d -Glc p -(1 → 6)-α- d -Glc p -(1 → 4)- d -Glc p )，水解α-1,6-糖苷键的还原端侧的α-1,4-糖苷键。panose 基序非还原端的结构对于酶识别底物很重要，并且底物特异性是独特的，与以前报道的酶不同。该酶催化panose的水解，具有kcat /K m为31.2 s -1 mM -1，催化作用导致异头倒转。这些酶促特性表明，该酶可与球孢芽孢杆菌N75 中的 1,4-α-葡聚糖 6-α-葡糖基转移酶很好地配对，从而有效地从淀粉中生产异麦芽糖。

  DOI：

  10.1016/j.carres.2022.108578
• 作为产物：
  描述：

  alkaline earth salt of/the/ methylsulfuric acid 在 pullulanase 、 yeast 、 sodium acetate 作用下， 以47%的产率得到alpha-D-苏-己糖吡喃糖苷-(1->4)-alpha-D-苏-己糖吡喃糖苷-(1->4)-alpha-D-苏-己糖吡喃糖苷-(1->6)-beta-D-苏-吡喃己糖
  参考文献：
  名称：

  Preparation of 63-α-D-glucosyimaltotriose

  摘要：

  DOI：

  10.1016/s0008-6215(00)84574-7

文献信息

• Synthesis of Glucose-Containing Linear Oligosaccharides Having<i>α</i>(1→4) and<i>α</i>(1→6) Linkages Using Stereoselective Dehydrative Glycosylation
  作者：Shinkiti Koto、Hisamitsu Haigoh、Sonoko Shichi、Motoko Hirooka、Teiko Nakamura、Chika Maru、Misuzu Fujita、Ayano Goto、Tomoko Sato、Masato Okada、Shonosuke Zen、Kazuo Yago、Fumiya Tomonaga

  DOI：10.1246/bcsj.68.2331

  日期：1995.8

  g linear tetra-, hexa-, and octasaccharides of D-glucose having α(1→4) and α(1→6) linkages were synthesized using a stereoselective dehydrative glycosylation with a reagent mixture of p-nitrobenzenesulfonyl chloride, silver trifluoromethanesulfonate, N,N-dimethylacetamide, and triethylamine. A cross-condensation of a quasi-stoichiometric amount of a donor and an acceptor of an octasaccharide, followed

  使用立体选择性脱水糖基化与对硝基苯磺酰基试剂混合物合成具有 α(1→4) 和 α(1→6) 键的 D-葡萄糖的与糖原储存相关的线性四糖、六糖和八糖氯化物、三氟甲磺酸银、N,N-二甲基乙酰胺和三乙胺。准化学计量量的八糖供体和受体交叉缩合，然后脱保护，得到葡糖十六糖。
• Dehydrating agent and method for dehydrating moist article using the agent and dehydrated article obtained by the method
  申请人：Kubota Michio

  公开号：US20060008791A1

  公开（公告）日：2006-01-12

  The present invention has the object to dehydrate hydrous matters without denaturing or deteriorating them by using a dehydrating agent comprising an anhydrous cyclotetrasaccharide, and provides a dehydrating agent comprising the cyclotetrasaccharide; a method for dehydrating hydrous matters through a step of incorporating, contacting or coexisting the cyclotetrasaccharide into, with, or in the hydrous matters; and dehydrated products obtainable thereby.

  本发明的目的是通过使用包含无水环四糖的脱水剂，将含水物质脱水而不使其变性或恶化，并提供包含环四糖的脱水剂；通过将环四糖并入、接触或共存于含水物质中的步骤来脱水含水物质的方法；以及由此获得的脱水产品。
• Gluco-oligomers initially formed by the reuteransucrase enzyme of Lactobacillus reuteri 121 incubated with sucrose and malto-oligosaccharides
  作者：Justyna M Dobruchowska、Xiangfeng Meng、Hans Leemhuis、Gerrit J Gerwig、Lubbert Dijkhuizen、Johannis P Kamerling

  DOI：10.1093/glycob/cwt048

  日期：2013.9

  The probiotic bacterium Lactobacillus reuteri 121 produces a complex, branched (1 → 4, 1 → 6)-α-d-glucan as extracellular polysaccharide (reuteran) from sucrose (Suc), using a single glucansucrase/glucosyltransferase (GTFA) enzyme (reuteransucrase). To gain insight into the reaction/product specificity of the GTFA enzyme and the mechanism of reuteran formation, incubations with Suc and/or a series of malto-oligosaccharides (MOSs) (degree of polymerization (DP2–DP6)) were followed in time. The structures of the initially formed products, isolated via high-performance anion-exchange chromatography, were analyzed by matrix-assisted laser-desorption ionization time-of-flight mass spectrometry and 1D/2D 1H/13C NMR spectroscopy. Incubations with Suc only, acting as both donor and acceptor, resulted in elongation of Suc with glucose (Glc) units via alternating (α1 → 4) and (α1 → 6) linkages, yielding linear gluco-oligosaccharides up to at least DP ∼ 12. Simultaneously with the ensemble of oligosaccharides, polymeric material was formed early on, suggesting that alternan fragments longer than DP ∼ 12 have higher affinity with the GTFA enzyme and are quickly extended, yielding high-molecular-mass branched reuteran (4 × 107 Da). MOSs (DP2–DP6) in the absence of Suc turned out to be poor substrates. Incubations of GTFA with Suc plus MOSs as substrates resulted in preferential elongation of MOSs (acceptors) with Glc units from Suc (donor). This apparently reflects the higher affinity of GTFA for MOSs compared with Suc. In accordance with the GTFA specificity, most prominent products were oligosaccharides with an (α1 → 4)/(α1 → 6) alternating structure.

  益生菌吕特氏乳杆菌（Lactobacillus reuteri）121利用一种单一的葡聚糖琥珀酸酶/葡糖基转移酶（GTFA）（reuteransucrase），从蔗糖（Suc）中产生一种复杂的支链（1→4，1→6）-α-d-葡聚糖作为胞外多糖（reuteran）。为了深入了解 GTFA 酶的反应/产物特异性和芦丁聚糖的形成机制，对 Suc 和/或一系列麦芽寡糖（MOS）（聚合度为 DP2-DP6）的孵育过程进行了及时跟踪。最初形成的产物通过高效阴离子交换色谱法分离出来，并通过基质辅助激光解吸电离飞行时间质谱法和 1D/2D 1H/13C NMR 光谱法分析其结构。在同时作为供体和受体的 Suc 诱导下，Suc 通过交替 (α1 → 4) 和 (α1 → 6) 连接与葡萄糖（Glc）单元发生伸长，产生线性葡萄糖寡糖，至少达到 DP ∼ 12。在低聚糖集合的同时，很早就形成了聚合物质，这表明长于 DP ∼ 12 的交替聚糖片段与 GTFA 酶的亲和力较高，并能迅速延伸，产生高分子质量的支链芦丁聚糖（4 × 107 Da）。在没有 Suc 的情况下，MOSs（DP2-DP6）是较差的底物。将 GTFA 与作为底物的 Suc 和 MOS 一起培养，结果是 MOS（接受者）优先与来自 Suc（供体）的 Glc 单位发生延伸。这显然反映出与 Suc 相比，GTFA 对 MOS 的亲和力更高。根据 GTFA 的特异性，最主要的产物是具有（α1 → 4）/（α1 → 6）交替结构的寡糖。
• Preparation of 63-α-D-glucosyimaltotriose
  作者：Barry V. McCleary

  DOI：10.1016/s0008-6215(00)84574-7

  日期：1980.10
• Discovery of a novel glucanohydrolase, 4-α-isomaltooligosylglucose 4-glucanohydrolase, that can be used for efficient production of isomaltose
  作者：Noriaki Kitagawa、Hikaru Watanabe、Tetsuya Mori、Hajime Aga、Shimpei Ushio、Koryu Yamamoto

  DOI：10.1016/j.carres.2022.108578

  日期：2022.7

  We discovered a novel enzyme in our pursuit of an improved method for the production of isomaltose. The enzyme, 4-α-isomaltooligosylglucose 4-glucanohydrolase from Sarocladium kiliense U4520, recognizes the panose motif (α-d-Glcp-(1 → 6)-α-d-Glcp-(1 → 4)-d-Glcp) and hydrolyzes the α-1,4-glucosidic bond on the reducing end side with respect to the α-1,6-glucosidic bond. The structure on the non-reducing

  我们在寻求改进异麦芽糖生产方法的过程中发现了一种新型酶。来自Sarocladium kiliense U4520的 4-α-异麦芽寡糖基葡萄糖 4-葡聚糖水解酶可识别泛糖基序 (α- d -Glc p -(1 → 6)-α- d -Glc p -(1 → 4)- d -Glc p )，水解α-1,6-糖苷键的还原端侧的α-1,4-糖苷键。panose 基序非还原端的结构对于酶识别底物很重要，并且底物特异性是独特的，与以前报道的酶不同。该酶催化panose的水解，具有kcat /K m为31.2 s -1 mM -1，催化作用导致异头倒转。这些酶促特性表明，该酶可与球孢芽孢杆菌N75 中的 1,4-α-葡聚糖 6-α-葡糖基转移酶很好地配对，从而有效地从淀粉中生产异麦芽糖。