N,N′-di-((2E)-3-(3,4-dihydroxyphenyl)acrylicacyl)-1,3,5-triazine-2,4,6-triamine

• 分子结构分类: 有机化合物 - 苯丙烷和聚酮 - 肉桂酸及其衍生物
• 英文名称: N,N′-di-((2E)-3-(3,4-dihydroxyphenyl)acrylicacyl)-1,3,5-triazine-2,4,6-triamine
• 英文别名: (E)-N-[4-amino-6-[[(E)-3-(3,4-dihydroxyphenyl)prop-2-enoyl]amino]-1,3,5-triazin-2-yl]-3-(3,4-dihydroxyphenyl)prop-2-enamide
• 化学式: C₂₁H₁₈N₆O₆
• 分子量: 450.411
• InChiKey: ZZRISVBGQXXUOK-FCXRPNKRSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  1.4
• 重原子数：
  33
• 可旋转键数：
  6
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.0
• 拓扑面积：
  204
• 氢给体数：
  7
• 氢受体数：
  10

反应信息

• 作为产物：
  描述：

  三聚氰胺 、 TRANS-咖啡酸 在 1-羟基苯并三唑 、 盐酸-N-乙基-Nˊ-(3-二甲氨基丙基)碳二亚胺 、 三乙胺 作用下， 以 二氯甲烷 、 N,N-二甲基甲酰胺 为溶剂， 生成 N,N′-di-((2E)-3-(3,4-dihydroxyphenyl)acrylicacyl)-1,3,5-triazine-2,4,6-triamine
  参考文献：
  名称：

  Dynamic and Reversible Fluorescence Imaging of Superoxide Anion Fluctuations in Live Cells and in Vivo

  摘要：

  Overgeneration of reactive oxygen species (ROS) is closely associated with cellular damage and diseases. As superoxide anion (O-2(center dot-)) is the precursor of other ROS, exploring O-2(center dot-). fluctuations in cells and in vivo is of great significance. To address this critical need, we have developed a novel reversible fluorescent probe with one-photon and two-photon fluorescence properties, which is well suited for monitoring O-2(center dot-) fluxes selectively and dynamically. Imaging results substantiate dynamic and reversible fluorescence responses of this probe to intracellular O-2(center dot-) under apoptotic stimuli. Moreover, this probe can conveniently visualize changes in O-2(center dot-). concentration during reperfusion injury in hepatocytes, zebrafish, and mice, by means of one-photon or two-photon imaging according to depths of various samples. The present study provides a powerful fluorescent imaging tool for dynamic tracking of O-2(center dot-). in live cells and in vivo.

  DOI：

  10.1021/ja408524j

文献信息

• Dynamic and Reversible Fluorescence Imaging of Superoxide Anion Fluctuations in Live Cells and in Vivo
  作者：Wen Zhang、Ping Li、Fan Yang、Xiufen Hu、Chuanzhi Sun、Wei Zhang、Dezhan Chen、Bo Tang

  DOI：10.1021/ja408524j

  日期：2013.10.9

  Overgeneration of reactive oxygen species (ROS) is closely associated with cellular damage and diseases. As superoxide anion (O-2(center dot-)) is the precursor of other ROS, exploring O-2(center dot-). fluctuations in cells and in vivo is of great significance. To address this critical need, we have developed a novel reversible fluorescent probe with one-photon and two-photon fluorescence properties, which is well suited for monitoring O-2(center dot-) fluxes selectively and dynamically. Imaging results substantiate dynamic and reversible fluorescence responses of this probe to intracellular O-2(center dot-) under apoptotic stimuli. Moreover, this probe can conveniently visualize changes in O-2(center dot-). concentration during reperfusion injury in hepatocytes, zebrafish, and mice, by means of one-photon or two-photon imaging according to depths of various samples. The present study provides a powerful fluorescent imaging tool for dynamic tracking of O-2(center dot-). in live cells and in vivo.