NSC4239

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 喹啉及其衍生物
• 英文名称: NSC4239
• 英文别名: N,N-dimethyl-4-[(Z)-2-(1-methylquinolin-1-ium-4-yl)ethenyl]aniline
• 化学式: C₂₀H₂₁N₂
• mdl: MFCD02692162
• 分子量: 289.4
• InChiKey: KKVCEMBXOHNBRG-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  4.7
• 重原子数：
  22
• 可旋转键数：
  3
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.15
• 拓扑面积：
  7.1
• 氢给体数：
  0
• 氢受体数：
  1

反应信息

• 作为产物：
  描述：

  4-甲基喹啉 在 哌啶 、 溶剂黄146 作用下， 以 乙醇 为溶剂， 反应 12.0h， 生成 NSC4239
  参考文献：
  名称：

  工程化基于双转子的荧光分子，以高信噪比（S / B）敏感地跟踪活细胞和斑马鱼中的线粒体粘度

  摘要：

  已经提出了一种创新的设计策略，以基于双转子分子平台构建具有出色灵敏度和高信噪比（S / B）的粘度敏感荧光探针。作为概念验证，开发了一个包含两个转子（喹啉鎓和咔唑部分）的基于双转子的荧光分子（DRVP），以使用强大的ICT平台跟踪粘度。阳离子探针DRVP能够显示活细胞和斑马鱼中线粒体的粘度变化。

  DOI：

  10.1016/j.jphotochem.2020.112789

文献信息

• FLUORESCENT MOLECULAR ROTORS
  申请人：AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

  公开号：US20160195519A1

  公开（公告）日：2016-07-07

  The present invention relates to methods and compositions for detecting an interaction between a protein and a ligand, comprising: (i) binding at least one fluorescent molecular rotor to said ligand or protein; and (ii) detecting a change in fluorescence emitted by said fluorescent molecular rotor after contact of the bound fluorescent molecular rotor with the other of said ligand or protein, thereby detecting an interaction between the ligand and the protein, wherein the fluorescent molecular rotor comprises: a rotating ?-bond; an electron-donating moiety; an electron-accepting moiety; and a ?-conjugated linker.

  本发明涉及一种检测蛋白质与配体之间相互作用的方法和组合物，包括：(i)将至少一个荧光分子转子结合到所述配体或蛋白质上；和(ii)在所述结合的荧光分子转子与所述配体或蛋白质中的另一个接触后检测所述荧光分子转子发射的荧光变化，从而检测配体和蛋白质之间的相互作用，其中所述荧光分子转子包括：一个旋转的π键；一个给电子的基团；一个受电子的基团；和一个π共轭连接物。
• DYES FOR ANALYSIS OF PROTEIN AGGREGATION
  申请人：Patton Wayne Forrest

  公开号：US20130137112A1

  公开（公告）日：2013-05-30

  Provided are dyes and compositions which are useful in a number of applications, such as the detection and monitoring protein aggregation, kinetic studies of protein aggregation, neurofibrillary plaques analysis, evaluation of protein formulation stability, and analysis of molecular chaperone activity.

  提供了染料和组合物，可用于许多应用，如检测和监测蛋白质聚集，蛋白质聚集动力学研究，神经纤维缠结斑点分析，评估蛋白质配方稳定性和分子伴侣活性分析。
• Methods for dye selection for protein melt temperature determinations
  申请人：LIFE TECHNOLOGIES CORPORATION

  公开号：US10024863B2

  公开（公告）日：2018-07-17

  According to the present teachings, compositions, kits, and methods for protein melt analysis are provided that utilizing one or more fluorophore dyes. In some embodiments, a method comprises preparing a sample by mixing at least one protein with two or more dyes, and applying a controlled heating, while recording the fluorescence emission of the sample. The methods can be used, for example, for screening conditions for optimized protein stability, screening for ligands that bind and enhance protein stability (e.g., protein-protein interactions), screening for mutations for enhanced stability, screening crystallization conditions for protein stability, screening storage conditions for protein stability, and screening conditions in which a protein will be used (e.g., production conditions, treatment conditions, etc.) for protein stability.

  根据本教导，提供了利用一种或多种荧光团染料进行蛋白质熔体分析的组合物、试剂盒和方法。在某些实施方案中，方法包括通过将至少一种蛋白质与两种或两种以上染料混合来制备样品，并进行受控加热，同时记录样品的荧光发射。这些方法可用于筛选优化蛋白质稳定性的条件、筛选结合并提高蛋白质稳定性的配体（如蛋白质-蛋白质相互作用）、筛选提高稳定性的突变、筛选提高蛋白质稳定性的结晶条件、筛选提高蛋白质稳定性的储存条件，以及筛选提高蛋白质稳定性的蛋白质使用条件（如生产条件、处理条件等）。
• METHODS FOR DYE SELECTION FOR PROTEIN MELT TEMPERATURE DETERMINATIONS
  申请人：LIFE TECHNOLOGIES CORPORATION

  公开号：US20140315190A1

  公开（公告）日：2014-10-23

  According to the present teachings, compositions, kits, and methods for protein melt analysis are provided that utilizing one or more fluorophore dyes. In some embodiments, a method comprises preparing a sample by mixing at least one protein with two or more dyes, and applying a controlled heating, while recording the fluorescence emission of the sample. The methods can be used, for example, for screening conditions for optimized protein stability, screening for ligands that bind and enhance protein stability (e.g., protein-protein interactions), screening for mutations for enhanced stability, screening crystallization conditions for protein stability, screening storage conditions for protein stability, and screening conditions in which a protein will be used (e.g., production conditions, treatment conditions, etc.) for protein stability.
• US9863937B2
  申请人：——

  公开号：US9863937B2

  公开（公告）日：2018-01-09