1-[5-(溴甲基)-2-硝基苯基]乙酮 | 99821-59-7

• 分子结构分类: 有机化合物 - 有机氧化合物
• 中文名称: 1-[5-(溴甲基)-2-硝基苯基]乙酮
• 英文名称: 1-(5-Bromomethyl-2-nitrophenyl)-ethanone
• 英文别名: 5-bromomethyl-2-nitroacetophenone；Ethanone, 1-[5-(bromomethyl)-2-nitrophenyl]-；1-[5-(bromomethyl)-2-nitrophenyl]ethanone
• CAS: 99821-59-7
• 化学式: C₉H₈BrNO₃
• 分子量: 258.071
• InChiKey: NBZVFFAEWDJGHN-UHFFFAOYSA-N

物化性质

• 沸点：
  395.8±32.0 °C(Predicted)
• 密度：
  1.590±0.06 g/cm3(Predicted)

计算性质

• 辛醇/水分配系数(LogP)：
  1.8
• 重原子数：
  14
• 可旋转键数：
  2
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.22
• 拓扑面积：
  62.9
• 氢给体数：
  0
• 氢受体数：
  3

安全信息

• 储存条件：
  2-8°C

反应信息

• 作为反应物：
  描述：

  1-[5-(溴甲基)-2-硝基苯基]乙酮 在 sodium azide 作用下， 生成 1-(5-azidomethyl-2-nitrophenyl)ethanone
  参考文献：
  名称：

  使用可分离珠粒和高密度过滤器对循环肿瘤细胞进行高效分离和准确原位分析

  摘要：

  癌细胞分析：一种新技术可以分离出稀有的循环肿瘤细胞（CTC），并通过使用可分离的珠子和高孔密度过滤器来分析其蛋白质表达。该技术在从外周血中分离稀有CTC方面显示出显着提高的效率，并能够准确测量原位蛋白表达水平。

  DOI：

  10.1002/anie.201302278
• 作为产物：
  描述：

  1-(5-甲基-2-硝基苯基)乙酮 在 N-溴代丁二酰亚胺(NBS) 、 1,1'-偶氮(氰基环己烷) 作用下， 以 乙腈 为溶剂， 以68 %的产率得到1-[5-(溴甲基)-2-硝基苯基]乙酮
  参考文献：
  名称：

  [EN] METHOD AND COMPOUND
  [FR] PROCÉDÉ ET COMPOSÉ

  摘要：

  Provided herein is a method for modifying a polynucleotide or an analogue or derivative thereof, comprising reacting the polynucleotide or analogue or derivative thereof with a compound of formula (I), wherein RH, L1, Ⓛ, R, n and RPare as defined herein. Also provided are associated methods for purifying a polynucleotide or analogue or derivative thereof, uses of a compound of formula (I), and such compounds per se.

  公开号：

  WO2023180237A1

文献信息

• Photocleavable isotope-coded affinity tags
  申请人：Olejnik Jerzy

  公开号：US20050085630A1

  公开（公告）日：2005-04-21

  This invention relates to agents and conjugates that can be used to detect and isolate target components from complex mixtures such as proteins and protein fragments from biological samples from in vivo and in vitro sources. Agents comprise a detectable group bound to a photoreactive group. Conjugates comprise agents coupled to substrates by covalent bounds which can be selectively cleaved with the administration of electromagnetic radiation. Targets substances labeled with detectable molecules can be easily identified and separated from a heterologous mixture of substances. Exposure of the conjugate to radiation releases the target in a functional form and completely unaltered. Using photocleavable molecular precursors as the conjugates, label can be incorporated into macromolecules, the nascent macromolecules isolated and the label completely removed. The invention also relates to targets isolated with these conjugates which may be useful as pharmaceutical agents or compositions that can be administered to humans and other mammals.

  本发明涉及可以用于检测和分离来自复杂混合物（如来自体内和体外源的生物样本中的蛋白质和蛋白质片段）的靶组分的试剂和结合物。试剂包括结合到光反应基团的可检测基团。结合物包括通过共价键结合到底物的试剂，这些共价键可以通过电磁辐射的施加而选择性地断裂。标记有可检测分子的目标物质可以很容易地从异源混合物中识别和分离出来。将结合物暴露于辐射会释放出目标物质以功能形式完全不变。使用光解分子前体作为结合物，标记可以被结合到大分子中，新生大分子被分离并标记完全去除。该发明还涉及使用这些结合物分离出的目标物质，这些目标物质可能作为药用剂或可用于给人类和其他哺乳动物的组合物。
• MASSIVE PARALLEL METHOD FOR DECODING DNA AND RNA
  申请人：The Trustees of Columbia University in the City of New York

  公开号：US20160264612A1

  公开（公告）日：2016-09-15

  This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.

  这项发明提供了一种将核酸连接到固体表面的方法，以及通过在聚合酶反应中将核苷酸类似物并入到DNA增长链中后检测每个核苷酸类似物的身份来测序核酸的方法。该发明还提供了包含独特标签的核苷酸类似物，通过可切割的连接剂连接到核苷酸类似物上，并且包含用于封闭去氧核糖的3′-位置上的—OH基团的可切割化学基团。
• Photocleavable bioreactive agents
  申请人：The Trustees of Boston University

  公开号：US20030190680A1

  公开（公告）日：2003-10-09

  This invention relates to agents and conjugates that can be used to detect and isolate target components from complex mixtures such as nucleic acids from biological samples, cells from bodily fluids, and nascent proteins from translation reactions. Agents comprise a detectable moiety bound to a photoreactive moiety. Conjugates comprise agents coupled to substrates by covalent bounds which can be selectively cleaved with the administration of electromagnetic radiation. Targets substances labeled with detectable molecules can be easily identified and separated from a heterologous mixture of substances. Exposure of the conjugate to radiation releases the target in a functional form and completely unaltered. Using photocleavable molecular precursors as the conjugates, label can be incorporated into macromolecules, the nascent macromolecules isolated and the label completely removed. The invention also relates to targets isolated with these conjugates which may be useful as pharmaceutical agents or compositions that can be administered to humans and other mammals. Useful compositions include biological agents such as nucleic acids, proteins, lipids and cytokines. Conjugates can also be used to monitor the pathway and half-life of pharmaceutical composition in vivo and for diagnostic, therapeutic and prophylactic purposes. The invention also relates to kits comprised of agents and conjugates that can be used for the detection of diseases, disorders and nearly any individual substance in a complex background of substances.

  本发明涉及用于检测和分离复杂混合物中的目标成分的试剂和共轭物。这些混合物可以包括生物样品中的核酸、体液中的细胞以及翻译反应中的新生蛋白质等。这些试剂包括一个可检测的基团和一个光敏反应基团。共轭物由试剂和底物通过共价键结合而成，可以通过电磁辐射的作用选择性地断裂。标记有可检测分子的目标物质可以轻松地从异种混合物中识别和分离。将光解分子前体作为共轭物，可以将标记合并到大分子中，然后分离新生大分子并完全去除标记。本发明还涉及用这些共轭物分离的目标物质，这些目标物质可能作为药物或组成物对人类和其他哺乳动物有用。有用的组成物包括核酸、蛋白质、脂类和细胞因子等生物试剂。共轭物还可以用于监测体内药物组成物的途径和半衰期，以及用于诊断、治疗和预防目的。本发明还涉及试剂和共轭物组成的试剂盒，可用于检测疾病、紊乱和几乎任何复杂背景物质中的个体物质。
• Diagnosing genetic disorders
  申请人：The Trustees of Boston University

  公开号：US20030162198A1

  公开（公告）日：2003-08-28

  This invention relates to agents and conjugates that can be used to detect and isolate target components from complex mixtures such as nucleic acids from biological samples, cells from bodily fluids, and nascent proteins from translation reactions. Agents comprise a detectable moiety bound to a photoreactive moiety. Conjugates comprise agents coupled to substrates by covalent bounds which can be selectively cleaved with the administration of electromagnetic radiation. Targets substances labeled with detectable molecules can be easily identified and separated from a heterologous mixture of substances. Exposure of the conjugate to radiation releases the target in a functional form and completely unaltered. Using photocleavable molecular precursors as the conjugates, label can be incorporated into macromolecules, the nascent macromolecules isolated and the label completely removed. The invention also relates to targets isolated with these conjugates which may be useful as pharmaceutical agents or compositions that can be administered to humans and other mammals. Useful compositions include biological agents such as nucleic acids, proteins, lipids and cytokines. Conjugates can also be used to monitor the pathway and half-life of pharmaceutical composition in vivo and for diagnostic, therapeutic and prophylactic purposes. The invention also relates to kits comprised of agents and conjugates that can be used for the detection of diseases, disorders and nearly any individual substance in a complex background of substances.

  本发明涉及用于检测和分离复杂混合物中的目标组分的试剂和结合物，例如来自生物样品的核酸，来自体液的细胞和来自翻译反应的新生蛋白质。试剂包括与光反应性基团结合的可检测基团。结合物包括试剂通过共价键耦合到基质上，可以通过电磁辐射的给药选择性地断裂的结合物。标记有可检测分子的目标物质可以很容易地从异源混合物中识别和分离。将结合物暴露于辐射下会以功能形式完全不变地释放目标物质。使用光解分子前体作为结合物，可以将标记合并到大分子中，分离新生大分子并完全去除标记。本发明还涉及使用这些结合物分离的目标物质，可用作可用于人类和其他哺乳动物的药物剂或组合物。有用的组合物包括生物制剂，如核酸，蛋白质，脂质和细胞因子。结合物还可用于监测体内药物组合物的途径和半衰期，并用于诊断，治疗和预防目的。本发明还涉及由试剂和结合物组成的试剂盒，可用于检测疾病，障碍和几乎任何复杂背景物质中的个体物质。
• Detecting targets in heterologous mixtures
  申请人：The Trustees of Boston University

  公开号：US20040053217A1

  公开（公告）日：2004-03-18

  This invention relates to agents and conjugates that can be used to detect and isolate target components from complex mixtures such as nucleic acids from biological samples, cells from bodily fluids, and nascent proteins from translation reactions. Agents comprise a detectable moiety bound to a photoreactive moiety. Conjugates comprise agents coupled to substrates by covalent bounds which can be selectively cleaved with the administration of electromagnetic radiation. Targets substances labeled with detectable molecules can be easily identified and separated from a heterologous mixture of substances. Exposure of the conjugate to radiation releases the target in a functional form and completely unaltered. Using photocleavable molecular precursors as the conjugates, label can be incorporated into macromolecules, the nascent macromolecules isolated and the label completely removed. The invention also relates to targets isolated with these conjugates which may be useful as pharmaceutical agents or compositions that can be administered to humans and other mammals. Useful compositions include biological agents such as nucleic acids, proteins, lipids and cytokines. Conjugates can also be used to monitor the pathway and half-life of pharmaceutical composition in vivo and for diagnostic, therapeutic and prophylactic purposes. The invention also relates to kits comprised of agents and conjugates that can be used for the detection of diseases, disorders and nearly any individual substance in a complex background of substances.

  本发明涉及一种可用于检测和分离复杂混合物中的目标成分的试剂和共轭物，例如来自生物样本的核酸、来自体液的细胞和来自翻译反应的新生蛋白质。试剂包括结合到光反应性基团的可检测基团。共轭物包括通过共价键结合到基质的试剂，可以通过电磁辐射的给予选择性断裂。标记有可检测分子的目标物质可以轻松地从异种混合物中识别和分离。将光裂解的分子前体作为共轭物，标签可以被纳入大分子中，新生大分子可以被分离并完全去除标签。本发明还涉及使用这些共轭物分离的目标物质，这些物质可能作为药物或组成物对人类和其他哺乳动物有用。有用的组成物包括核酸、蛋白质、脂质和细胞因子等生物试剂。共轭物还可用于监测体内药物组成物的途径和半衰期，并用于诊断、治疗和预防目的。本发明还涉及试剂和共轭物组成的试剂盒，可用于检测疾病、障碍和几乎任何复杂背景物质中的个体物质。