3-β-Galactosyloxy-4-nitrobenzoic Acid | 78261-89-9

• 分子结构分类: 有机化合物 - 有机氧化合物
• 英文名称: 3-β-Galactosyloxy-4-nitrobenzoic Acid
• 英文别名: 4-nitro-3-[(2S,3R,4S,5R,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxybenzoic acid
• CAS: 78261-89-9
• 化学式: C₁₃H₁₅NO₁₀
• 分子量: 345.263
• InChiKey: KUFXWXCDFRSCAE-VFZGUZRASA-N

物化性质

• 沸点：
  691.2±55.0 °C(Predicted)
• 密度：
  1.718±0.06 g/cm3(Predicted)

计算性质

• 辛醇/水分配系数(LogP)：
  -1
• 重原子数：
  24
• 可旋转键数：
  4
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.46
• 拓扑面积：
  183
• 氢给体数：
  5
• 氢受体数：
  10

反应信息

• 作为反应物：
  描述：

  N-羟基丁二酰亚胺 、 3-β-Galactosyloxy-4-nitrobenzoic Acid 以 N,N-二甲基甲酰胺 为溶剂， 生成 甲基丙烯酸N-羟琥珀酸亚胺酯
  参考文献：
  名称：

  Charge effects in enzyme immunoassays

  摘要：

  一种确定特定结合对-配体和受体（抗配体）成员的方法。所使用的试剂包括提供由于存在多种离子电荷而产生电场的第一改性成员和用信号产生系统的组分标记的第二改性成员，该系统可能具有一个或多个组分。在测定介质中第一和第二改性成员的平均接近程度与分析物的量相关，其中信号产生系统的观察到的信号与电场对信号产生系统的影响相关。此外，提供了组合物，以及预定比例的试剂，用于优化对分析物浓度变化的信号响应。

  公开号：

  US04501692A1

文献信息

• Macromolecular glycosidase substrates
  申请人：SYVA COMPANY

  公开号：EP0018731A1

  公开（公告）日：1980-11-12

  Macromolecular compositions are provided which find use as glycosidase substrates, particularly where steric modulation of enzyme activity is involved. the compositions have a macromolecular hubto which is linked by means of a spacer arm a chromophore, which in turn is bonded to a sugar by an enzymatically labile glycosidyl linkage. The chromophore is chosen so as to undergo a substantial change in its chromophoric properties upon cleavage of the glycosidyl linkage. The enzyme activity can be determined spectrophotometrically due to the change in the absorptive or fluorescent properties of the chromophore upon cleavage of the glycosidyl linkage. Varying turnover rates for the enzyme are observed depending upon the nature of the macromolecular hub. the spacer arm, and the chromophore.

  提供了可用作糖苷酶底物的大分子组合物，特别是在涉及酶活性的立体调节时。组合物有一个大分子枢纽，它通过间隔臂连接一个发色团，而发色团又通过酶易失性糖苷键连接到糖上。选择发色团的目的是使其发色特性在糖苷键裂解时发生重大变化。由于发色团的吸收性或荧光性在糖苷键裂解时会发生变化，因此可以通过分光光度法确定酶的活性。根据大分子枢纽、间隔臂和发色团的性质，可观察到不同的酶周转率。
• Method of assaying substance and immunoassay element
  申请人：KONICA CORPORATION

  公开号：EP0328106A2

  公开（公告）日：1989-08-16

  Disclosed is a method of assaying a target substance in a fluid sample. In this method, a) the target substance, b) a substance which specifically binds to the target substance, to which a biological active substance which does not bind to the target substance is attached, or to which a substance which specifically binds to a biological active substance which does not bind to the target substance is attached; c) a labelled substance which is the target substance or an analogue thereof labelled with β-D-galactosidase, or which is a substance which specifically binds to the target substance, labelled with β-D-galactosidase, d) a substance which specifically binds to the biological active substance and which does not bind to the target substance, or the biological active substance, which is fixed to a carrier, which carrier exists in a porous reaction layer of an assay element, and e) a substance which specifically binds to β-D-galactosidase and which changes a signal originated from β-D-galactosidase, which is fixed to said carrier or another carrier which exists in a porous reaction layer of an assay element are reacted and the change of the signal from β-D-galactosidase is measured.

  本发明公开了一种检测流体样品中目标物质的方法。在该方法中，a) 目标物质；b) 与目标物质特异性结合的物质，其上附有不与目标物质结合的生物活性物质，或附有与不与目标物质结合的生物活性物质特异性结合的物质；c) 标记物质，该标记物质是标记有β-D-半乳糖苷酶的目标物质或其类似物，或 是标记有β-D-半乳糖苷酶的能与目标物质特异性结合的物质； d) 能与生物活性物质特异性结合但不能与目标物质或生物活性物质结合的物质，该物 质固定在载体上、e) 一种能与β-D-半乳糖苷酶特异性结合的物质，它能改变来自β-D-半乳糖苷酶的信号，该物质固定在所述载体上或存在于检测元件多孔反应层中的另一种载体上，两者发生反应，并测量来自β-D-半乳糖苷酶的信号的变化。
• EP0022768A4
  申请人：——

  公开号：EP0022768A4

  公开（公告）日：1980-08-18
• MACROMOLECULAR ENVIRONMENT CONTROL IN SPECIFIC RECEPTOR ASSAYS
  申请人：SYVA COMPANY

  公开号：EP0022768A1

  公开（公告）日：1981-01-28
• US4268663A
  申请人：——

  公开号：US4268663A

  公开（公告）日：1981-05-19