(11aS)-11-hydroxy-7-methoxy-8-([3-{5-[5-(5-methoxycarbonyl)-1-methyl-1H-pyrrol-3-ylcarbamoyl]-methyl-1H-pyrrol-3-ylcarbamoyl}-1-methyl-1H-pyrrol-3-ylcarbamoyl]-ethoxy)-5-oxo-2,3,11,11a-tetrahydro-1H,5H-pyrrolo[2,1-c][1,4]benzodiazepine-10-carboxylic acid tert-butyl ester | 909415-12-9

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 苯二氮卓类
• 英文名称: (11aS)-11-hydroxy-7-methoxy-8-([3-{5-[5-(5-methoxycarbonyl)-1-methyl-1H-pyrrol-3-ylcarbamoyl]-methyl-1H-pyrrol-3-ylcarbamoyl}-1-methyl-1H-pyrrol-3-ylcarbamoyl]-ethoxy)-5-oxo-2,3,11,11a-tetrahydro-1H,5H-pyrrolo[2,1-c][1,4]benzodiazepine-10-carboxylic acid tert-butyl ester
• 英文别名: tert-butyl (6S,6aS)-6-hydroxy-2-methoxy-3-[3-[[5-[[5-[(5-methoxycarbonyl-1-methylpyrrol-3-yl)carbamoyl]-1-methylpyrrol-3-yl]carbamoyl]-1-methylpyrrol-3-yl]amino]-3-oxopropoxy]-11-oxo-6a,7,8,9-tetrahydro-6H-pyrrolo[2,1-c][1,4]benzodiazepine-5-carboxylate
• CAS: 909415-12-9
• 化学式: C₄₀H₄₈N₈O₁₁
• 分子量: 816.868
• InChiKey: OGHOPQDECWINTQ-UGOHAPBYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  2
• 重原子数：
  59
• 可旋转键数：
  14
• 环数：
  6.0
• sp3杂化的碳原子比例：
  0.4
• 拓扑面积：
  217
• 氢给体数：
  4
• 氢受体数：
  11

反应信息

• 作为反应物：
  描述：

  (11aS)-11-hydroxy-7-methoxy-8-([3-{5-[5-(5-methoxycarbonyl)-1-methyl-1H-pyrrol-3-ylcarbamoyl]-methyl-1H-pyrrol-3-ylcarbamoyl}-1-methyl-1H-pyrrol-3-ylcarbamoyl]-ethoxy)-5-oxo-2,3,11,11a-tetrahydro-1H,5H-pyrrolo[2,1-c][1,4]benzodiazepine-10-carboxylic acid tert-butyl ester 在 三氟乙酸 、 碳酸氢钠 作用下， 以 水 为溶剂， 反应 3.0h， 以96%的产率得到(11aS) methyl 4-{[4-({4-[4-(7-methoxy-5-oxo-2,3,5,11a-tetrahydro-5H-pyrrolo[2,1-c][1,4]benzodiazepine-8-yloxy)propionylamino]-1-methyl-1H-pyrrole-2-carbonyl}-amino)-1-methyl-1H-pyrrole-2-carbonyl]amino}-1-methyl-1H-pyrrole-2-carboxylate
  参考文献：
  名称：

  WO2007/39752

  摘要：

  公开号：
• 作为产物：
  描述：

  L-脯氨醇 在 palladium on activated charcoal 4-二甲氨基吡啶 、 重铬酸吡啶 、 4 A molecular sieve 、 氢气 、 盐酸-N-乙基-Nˊ-(3-二甲氨基丙基)碳二亚胺 、 三乙胺 、 tin(ll) chloride 作用下， 以 四氢呋喃 、 甲醇 、 乙醇 、 二氯甲烷 、 N,N-二甲基甲酰胺 为溶剂， -30.0~20.0 ℃ 、110.32 kPa 条件下， 反应 50.0h， 生成 (11aS)-11-hydroxy-7-methoxy-8-([3-{5-[5-(5-methoxycarbonyl)-1-methyl-1H-pyrrol-3-ylcarbamoyl]-methyl-1H-pyrrol-3-ylcarbamoyl}-1-methyl-1H-pyrrol-3-ylcarbamoyl]-ethoxy)-5-oxo-2,3,11,11a-tetrahydro-1H,5H-pyrrolo[2,1-c][1,4]benzodiazepine-10-carboxylic acid tert-butyl ester
  参考文献：
  名称：

  Design, Synthesis, and Biophysical and Biological Evaluation of a Series of Pyrrolobenzodiazepine−Poly(N-methylpyrrole) Conjugates

  摘要：

  A novel series of methyl ester-terminated C8-linked pyrrolobenzodiazepine (PBD)-poly(N-methylpyrrole) conjugates (50a-f) has been synthesized and their DNA interaction evaluated by thermal denaturation, DNA footprinting, and in vitro transcription stop assays. The synergistic effect of attaching a PBD unit to a polypyrrole fragment is illustrated by the large increase in DNA binding affinity (up to 50-fold) compared to the individual PBD and pyrrole components. 50a-f were found to bind mainly to identical DNA sequences but with apparent binding site widths increasing with molecular length and the majority of sites conforming to the consensus motif 5'-XGXW(z) (z = 3 +/- 1; W = A or T; X = any base but preferably a purine). They also provided robust sequence-selective blockade of transcription at sites corresponding approximately to their DNA footprints. 50a-f were shown to have good cellular/ nuclear penetration properties, and a degree of correlation between cytotoxicity and DNA-binding affinity was observed.

  DOI：

  10.1021/jm051199z

文献信息

• WO2007/39752
  申请人：——

  公开号：——

  公开（公告）日：——
• Design, Synthesis, and Biophysical and Biological Evaluation of a Series of Pyrrolobenzodiazepine−Poly(<i>N</i>-methylpyrrole) Conjugates
  作者：Geoff Wells、Christopher R. H. Martin、Philip W. Howard、Zara A. Sands、Charles A. Laughton、Arnaud Tiberghien、Chi Kit Woo、Luke A. Masterson、Marissa J. Stephenson、John A. Hartley、Terence C. Jenkins、Steven D. Shnyder、Paul M. Loadman、Michael J. Waring、David E. Thurston

  DOI：10.1021/jm051199z

  日期：2006.9.1

  A novel series of methyl ester-terminated C8-linked pyrrolobenzodiazepine (PBD)-poly(N-methylpyrrole) conjugates (50a-f) has been synthesized and their DNA interaction evaluated by thermal denaturation, DNA footprinting, and in vitro transcription stop assays. The synergistic effect of attaching a PBD unit to a polypyrrole fragment is illustrated by the large increase in DNA binding affinity (up to 50-fold) compared to the individual PBD and pyrrole components. 50a-f were found to bind mainly to identical DNA sequences but with apparent binding site widths increasing with molecular length and the majority of sites conforming to the consensus motif 5'-XGXW(z) (z = 3 +/- 1; W = A or T; X = any base but preferably a purine). They also provided robust sequence-selective blockade of transcription at sites corresponding approximately to their DNA footprints. 50a-f were shown to have good cellular/ nuclear penetration properties, and a degree of correlation between cytotoxicity and DNA-binding affinity was observed.