tetranitro blue tetrazolium chloride | 1184-43-6

• 物质功能分类: 生物化工 - 生化试剂 - 生物染料
• 分子结构分类: 有机化合物 - 苯类化合物 - 苯和取代衍生物
• 英文名称: tetranitro blue tetrazolium chloride
• 英文别名: TNBT；Tetranitro-BT；2-[4-[4-[3,5-bis(4-nitrophenyl)tetrazol-3-ium-2-yl]-3-methoxyphenyl]-2-methoxyphenyl]-3,5-bis(4-nitrophenyl)tetrazol-3-ium;chloride
• CAS: 1184-43-6
• 化学式: C₄₀H₂₈N₁₂O₁₀*2Cl
• 分子量: 907.643
• InChiKey: VHGSPFITCDCEAV-UHFFFAOYSA-M

物化性质

• 熔点：
  ~170 °C (dec.)
• 溶解度：
  易溶于水、乙醇、甲醇、N,N二甲基甲酰胺
• 最大波长(λmax)：
  279 nm
• 稳定性/保质期：

  如果按照规定使用和储存，则不会发生分解，没有已知的危险反应，应避免接触氧化物。

计算性质

• 辛醇/水分配系数(LogP)：
  3.06
• 重原子数：
  63
• 可旋转键数：
  9
• 环数：
  8.0
• sp3杂化的碳原子比例：
  0.05
• 拓扑面积：
  271
• 氢给体数：
  0
• 氢受体数：
  15

安全信息

• TSCA：
  Yes
• 危险等级：
  4.1
• 危险品标志：
  T
• 安全说明：
  S53
• 危险类别码：
  R45
• WGK Germany：
  3

制备方法与用途

用途：用于琥珀酸脱氢酶（succinate dehydrogenase）在细胞化学研究中的应用。

反应信息

• 作为反应物：
  描述：

  噻唑偶氮苯二酚 、 tetranitro blue tetrazolium chloride 在 ammonium hydroxide 、 溶剂黄146 作用下， 以 氯仿 、 水 为溶剂， 生成 (TNBT²⁺)(HTAR^-)₂
  参考文献：
  名称：

  Ion-association between some ditetrazolium cations and anions originated from 4-(2-thiazolylazo)resorcinol or 4-(2-pyridylazo)resorcinol

  摘要：

  Ion-associates between cations originating from ditetrazolium salts [DT: Neotetrazolium chloride (NT), Blue Tetrazolium chloride (BT), Nitro Blue Tetrazolium chloride (NBT), and Tetranitro Blue Tetrazolium chloride (TNBT)] and anions originating from azo compounds [AC: 4-(2-pyridylazo)resorcinol (H(2)PAR) and 4-(2-thiazolylazo)resorcinol (H(2)TAR)] were studied by water-chloroform extraction and spectrophotometry. Some key characteristics of the extracted compounds were determined. They can be expressed by different formulae depending on the ACs and DTs used: (1) (DT+)(HPAR(-)) (for DT = BT, NBT or TNBT); (2) (DT2+)(HTAR(-))(2) (for DT = BT, NBT or TNBT); and (3) (DT+)(2)(HAC(-))(2) (for DT = NT).

  DOI：

  10.1134/s1070363215010338

文献信息

• Phenylmethoxybis(tetrazolium) ion-association complexes with an anionic indium(III) — 4-(2-pyridylazo)resorcinol chelate
  作者：Teodora Stefanova、Kiril Gavazov

  DOI：10.2478/s11532-012-0149-4

  日期：2013.2.1

  Complex formation and liquid-liquid extraction were studied in systems containing indium(III), 4-(2-pyridylazo)resorcinol (PAR), phenylmethoxybis(tetrazolium) salt (MBT), water and chloroform. The following MBTs, which differ only by the number of -NO2 groups in their cationic parts, were used: 3,3′-(3,3′-dimetoxy-4,4′-biphenylene)bis(2,5-diphenyl-2H-tetrazolium chloride) (Blue Tetrazolium chloride, BT), 3,3′-(3,3′-dimetoxy-4,4′-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride] (Nitro Blue Tetrazolium chloride, NBT) and 3,3′-(3,3′-dimetoxy-4,4′-biphenylene)bis[2,5-di(4-nitrophenyl)-2H-tetrazolium chloride] (Tetranitro Blue Tetrazolium chloride, TNBT). The composition of the formed ternary complexes was determined, In:PAR:MBT=1:2:2, and the optimum conditions for their extraction found: pH, shaking time, concentration of the reagents and the sequence of their addition. Some key constants were estimated: constants of extraction (Kex), constants of association (β) and constants of distribution (KD). BT appears to be the best MBT for extraction of the In(III)-PAR species, [In3+(OH)3(PAR)2]4−, (Log Kex=10.9, Log β=9.8, Log KD=1.12, R%=92.7%). Several additional characteristics concerning its application as extraction-spectrophotometric reagent were calculated: limit of detection (LOD = 0.12 µg cm−3), limit of quantification (LOD = 0.40 µg cm−3) and Sandell’s sensitivity (SS =1.58 ng cm−2); Beer’s law is obeyed for In(III) concentrations up to 3.2 µg mL−1 with a molar absorptivity coefficient of 7.3×104 L mol−1 cm−1 at λmax=515 nm.

  摘要：研究了含铟(III)、4-(2-吡啶偶氮)邻苯二酚（PAR）、苯甲氧基双(四唑)盐（MBT）、水和氯仿的体系中的复合物形成和液液萃取。使用了以下仅在它们阳离子部分的-NO2基团数量上有所不同的MBT：3,3′-(3,3′-二甲氧基-4,4′-联苯)双(2,5-二苯基-2H-四唑氯化物)（蓝四唑氯化物，BT）、3,3′-(3,3′-二甲氧基-4,4′-联苯)双[2-(4-硝基苯基)-5-苯基-2H-四唑氯化物]（硝基蓝四唑氯化物，NBT）和3,3′-(3,3′-二甲氧基-4,4′-联苯)双[2,5-二(4-硝基苯基)-2H-四唑氯化物]（四硝基蓝四唑氯化物，TNBT）。确定了形成的三元复合物的组成，In:PAR:MBT=1:2:2，并找到了其萃取的最佳条件：pH值、摇动时间、试剂浓度以及它们添加的顺序。估计了一些关键常数：萃取常数（Kex）、结合常数（β）和分配常数（KD）。BT似乎是最适合提取In(III)-PAR物种，[In3+(OH)3(PAR)2]4−的MBT（Log Kex=10.9，Log β=9.8，Log KD=1.12，R%=92.7%）。计算了几个关于其作为萃取-光度试剂的应用的额外特征：检测限（LOD = 0.12 µg cm−3），定量限（LOD = 0.40 µg cm−3）和桑德尔灵敏度（SS =1.58 ng cm−2）；在λmax=515 nm处，对In(III)浓度达到3.2 µg mL−1的情况下，符合比尔定律，摩尔吸光系数为7.3×104 L mol−1 cm−1。