p-nitrophenyl α-D-rhamnopyranoside | 186833-49-8

• 分子结构分类: 有机化合物 - 有机氧化合物
• 英文名称: p-nitrophenyl α-D-rhamnopyranoside
• 英文别名: p-Nitro-phenyl alpha-d-rhamnopyranoside；(2R,3S,4S,5S,6R)-2-methyl-6-(4-nitrophenoxy)oxane-3,4,5-triol
• CAS: 186833-49-8
• 化学式: C₁₂H₁₅NO₇
• 分子量: 285.254
• InChiKey: YILIDCGSXCGACV-OVBJLEGISA-N

计算性质

• 辛醇/水分配系数(LogP)：
  0.1
• 重原子数：
  20
• 可旋转键数：
  2
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.5
• 拓扑面积：
  125
• 氢给体数：
  3
• 氢受体数：
  7

反应信息

• 作为反应物：
  描述：

  p-nitrophenyl α-D-rhamnopyranoside 、 ethyl 1-thio-α-D-rhamnopyranoside 在 sodium citrate buffer 、 Jack bean α-mannosidase 作用下， 以 乙腈 为溶剂， 反应 5.0h， 以32.1%的产率得到ethyl α-D-rhamnopyranosyl-(1->2)-1-thio-α-D-rhamnopyranoside
  参考文献：
  名称：

  α-甘露糖苷酶催化的（1→2）-α-d-鼠李糖二糖衍生物的合成

  摘要：

  摘要以对硝基苯基α-d-鼠李糖吡喃糖苷为糖基供体，以6当量的乙基1-硫代-α-d-鼠李糖吡喃糖苷为糖基受体进行酶促糖基化反应，得到了ad-鼠李糖寡糖衍生物。在25°C下，在0.1 M柠檬酸钠缓冲液（pH 4.5）–MeCN的1：1（v / v）混合物中，用菜豆α-甘露糖苷酶催化反应。该酶对反应显示出高催化活性，以32.1％的分离产率（基于供体底物）提供乙基α-d-鼠李吡喃糖基-（1→2）-1-硫代α-d-鼠李糖吡喃糖苷，其衍生物假单胞菌的抗原性脂多糖的常见寡糖单元。

  DOI：

  10.1016/j.carres.2004.01.015
• 作为产物：
  描述：

  p-nitrophenyl 2,3,4-tri-O-acetyl-α-D-rhamnopyranoside 在 sodium methylate 作用下， 以 甲醇 为溶剂， 反应 2.0h， 以72.4%的产率得到p-nitrophenyl α-D-rhamnopyranoside
  参考文献：
  名称：

  Hydrolytic Activity ofα-Mannosidase against Deoxy Derivatives ofp-Nitrophenylα-d-Mannopyranoside

  摘要：

  合成了对硝基苯（PNP）α-d-吡喃甘露糖苷的脱氧衍生物、PNP 2-脱氧-α-d-阿拉伯诺-吡喃己糖苷、3-脱氧-α-d-阿拉伯诺-吡喃己糖苷、4-脱氧-α-d-来苏诺-吡喃己糖苷和α-d-鼠李糖苷，并研究了千层豆和杏仁α-甘露糖苷酶对它们的水解活性。这些α-甘露糖苷酶几乎不作用于 2-、3-和 4-脱氧衍生物，而 6-脱氧衍生物被酶水解的速度与 PNP α-d-吡喃甘露糖苷一样快，后者是α-甘露糖苷酶的常见底物。这些结果表明，曼吡喃糖苷的 C-2、3 和 4 处的羟基是这些酶识别底物的必要条件，而 C-6 处的羟基在底物鉴别中并没有如此关键的作用。动力学研究得出了酶水解 PNP α-d-rhamnopyranoside 的 Km 值和 Vmax 值。

  DOI：

  10.1271/bbb.60.2038

文献信息

• Biological indicator
  申请人：American Sterilizer Company

  公开号：US10011843B2

  公开（公告）日：2018-07-03

  This invention relates to a biological indicator derived from a composition comprising: a host organism comprising a spore forming bacteria; a reporter gene for producing an indicator enzyme; a regulatory gene; and a vehicle for inserting the reporter gene and the regulatory gene in the host organism; the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene; the insertion sequence comprising a transposase, a pair of terminal inverted repeat sequences, and at least one open reading frame for expressing the transposase. The vehicle may be taken up by the host organism. The insertion sequence may be inserted in the regulatory gene. The host organism may undergo sporulation to form the biological indicator. A process and an apparatus for using the biological indicator are disclosed.

  本发明涉及一种生物指示剂，其成分包括：由形成孢子的细菌组成的宿主生物；产生指示剂酶的报告基因；调控基因；以及将报告基因和调控基因插入宿主生物的载体；宿主生物的基因组中含有转座遗传因子，用于在调控基因中插入插入序列；插入序列包括转座酶、一对末端倒置重复序列和至少一个用于表达转座酶的开放阅读框。载体可被宿主生物吸收。插入序列可插入调控基因中。宿主生物可进行孢子繁殖以形成生物指示剂。本发明公开了一种使用生物指示剂的工艺和装置。
• Method for detecting Escherichia coli in a sample
  申请人：3M INNOVATIVE PROPERTIES COMPANY

  公开号：US10023898B2

  公开（公告）日：2018-07-17

  A method of detecting Listeria monocytogenes. The method comprises providing a culture device with a selective culture medium and a detection article comprising a first indicator system. The selective culture medium facilitates the growth of Listeria microorganisms. When a Listeria microorganism is detected in a sample contacted with the culture medium, the detection article is contacted with the culture medium to detect Listeria monocytogenes.

  一种检测李斯特菌的方法。该方法包括提供一个带有选择性培养基的培养装置和一个包含第一指示系统的检测物品。选择性培养基有利于李斯特菌微生物的生长。当在与培养基接触的样品中检测到李斯特菌微生物时，检测物品与培养基接触以检测单增李斯特菌。
• Method for detecting a Shigella or Cronobacter microorganism
  申请人：3M INNOVATIVE PROPERTIES COMPANY

  公开号：US10619181B2

  公开（公告）日：2020-04-14

  A method of detecting a Shigella or Cronobacter microorganism. The method comprises providing a culture device with a selective culture medium and a detection article comprising a first indicator system.

  一种检测志贺氏菌或克罗诺斯菌微生物的方法。该方法包括提供一个带有选择性培养基的培养装置和一个包含第一指示系统的检测物品。
• Biological disinfection or sterilization indicator
  申请人：TERRAGENE LLC

  公开号：US11001799B2

  公开（公告）日：2021-05-11

  A biological indicator for testing the efficacy of sterilization or disinfection processes of closed environments or confined spaces, the biological indicator comprising a carrier; biological material supported on or embedded in the carrier; and a carrier container comprising a body and a cap. The carrier is fixed within an inner space of the cap, the cap being able to be placed in an open position completely exposing the carrier and the biological material to the closed environment or confined space to be disinfected. A method for determine the efficacy of a process of sterilization or disinfection of closed environments or confined spaces, preferably by means of an enzyme and a fluorescence compound, using said biological indicator.

  一种生物指示器，用于检测封闭环境或密闭空间的灭菌或消毒过程的功效，该生物指示器包括一个载体；支撑在载体上或嵌入载体的生物材料；以及一个由主体和盖子组成的载体容器。载体固定在盖子的内部空间中，盖子可置于打开位置，使载体和生物材料完全暴露在要消毒的封闭环境或密闭空间中。一种确定封闭环境或密闭空间灭菌或消毒过程有效性的方法，最好是通过酶和荧光化合物，使用所述生物指示剂。
• Rapid read-out biological indicator
  申请人：Minnesota Mining and Manufacturing Company to 3M Innovative Properties Company

  公开号：US20030077688A1

  公开（公告）日：2003-04-24

  A rapid method of determining the efficacy of a sterilization cycle, and an indicator adapted to perform such method, comprising subjecting to the sterilization cycle a source of active enzyme having activity which correlates with the viability of a microorganism commonly used to monitor sterilization, and incubating the enzyme source, following the completion of the sterilization cycle, with an effective amount of a substrate system capable of reacting with any residual active enzyme to produce a detectable enzyme-modified product.

  一种快速确定灭菌周期功效的方法，以及一种适用于执行该方法的指示剂，包括将活性酶源置于灭菌周期中，该酶源的活性与通常用于监测灭菌的微生物的活力相关；在灭菌周期完成后，将酶源与有效量的底物系统孵育，该底物系统能够与任何残留的活性酶发生反应，产生可检测的酶修饰产物。