Suggested is a method of obtaining a composition for inhibiting unwanted growth of human hair and/or increasing pigmentation of human hair and/or increasing pigmentation of human skin and/or increasing the level of CE protein components, comprising or consisting of the following steps:
(i) extracting viable, freeze-dried or dried cells of Tetraselmis sp., preferably Tetraselmis suecica, with water and optionally a second liquid extractant selected from the group consisting of hexane, ethyl acetate, ethanol, methanol, isopropanol, and
(ii) optionally adjusting the phototoxicity of the composition to a phototoxicity index of less than 5,
wherein the extraction comprises
(i-a) exposition of the cell material to the extractant for up to 24 h at a temperature of not more than 50 °C, and
(i-b) removal of the cell material to obtain an extract, the extract being the composition or being further processed into the composition.
建议一种获得用于抑制人类毛发不希望生长和/或增加人类毛发色素和/或增加人类皮肤色素和/或增加 CE 蛋白成分
水平的组合物的方法,包括或由以下步骤组成:
(i) 用
水和选自己烷、
乙酸乙酯、
乙醇、
甲醇、
异丙醇组成的组的第二液体萃取剂萃取有活力的冻干或干燥的四膜藻类细胞,最好是四膜藻类(Tetraselmis suecica)细胞,以及
(ii) 可选地将组合物的光毒性调整至光毒性指数小于 5、
其中萃取包括
(i-a) 在不超过 50 °C 的温度下,将细胞材料暴露于萃取剂达 24 小时,和
(i-b) 去除细胞材料以获得
提取物,
提取物即为组合物或进一步加工成组合物。