| 1117751-26-4

• 分子结构分类: 有机化合物 - 有机氧化合物
• CAS: 1117751-26-4
• 化学式: C₆₁H₈₀N₄O₃₁
• 分子量: 1365.31
• InChiKey: ZYNPIVAZHVRDKI-CDGZQOEASA-N

计算性质

• 辛醇/水分配系数(LogP)：
  1.63
• 重原子数：
  96.0
• 可旋转键数：
  38.0
• 环数：
  5.0
• sp3杂化的碳原子比例：
  0.62
• 拓扑面积：
  433.16
• 氢给体数：
  1.0
• 氢受体数：
  32.0

反应信息

• 作为反应物：
  描述：

  在 10% palladium on activated charcoal 、 氢气 作用下， 以 四氢呋喃 、 乙醇 为溶剂， 反应 1.0h， 生成
  参考文献：
  名称：

  Identification of trimannoside-recognizing peptide sequences from a T7 phage display screen using a QCM device

  摘要：

  Here, we report on the identification of trimannoside-recognizing peptide sequences from a T7 phage display screen using a quartz-crystal microbalance (QCM) device. A trimannoside derivative that can form a self-assembled monolayer (SAM) was synthesized and used for immobilization on the gold electrode surface of a QCM sensor chip. After six sets of one-cycle affinity selection, T7 phage particles displaying PSVGLFTH (8-mer) and SVGLGLGFSTVNCF (14-mer) were found to be enriched at a rate of 17/44, 9/44, respectively, suggesting that these peptides specifically recognize trimannoside. Binding checks using the respective single T7 phage and synthetic peptide also confirmed the specific binding of these sequences to the trimannoside-SAM. Subsequent analysis revealed that these sequences correspond to part of the primary amino acid sequence found in many mannose- or hexose- related proteins. Taken together, these results demonstrate the effectiveness of our T7 phage display environment for affinity selection of binding peptides. We anticipate this screening result will also be extremely useful in the development of inhibitors or drug delivery systems targeting polysaccharides as well as further investigations into the function of carbohydrates in vivo. (c) 2008 Elsevier Ltd. All rights reserved.

  DOI：

  10.1016/j.bmc.2008.11.004
• 作为产物：
  描述：

  (2R,3R,4S,5S,6S)-2-(acetoxymethyl)-6-(((2R,3R,4S,5S,6S)-6-(3-aminopropoxy)-3,5-bis(benzoyloxy)-4-(((2R,3S,4S,5R,6R)-3,4,5-triacetoxy-6-(acetoxymethyl)tetrahydro-2H-pyran-2-yl)oxy)tetrahydro-2H-pyran-2-yl)methoxy)tetrahydro-2H-pyran-3,4,5-triyl triacetate 、 14-azido-3,6,9,12-tetraoxatetradecanoic acid 在 1-羟基苯并三唑 、 盐酸-N-乙基-Nˊ-(3-二甲氨基丙基)碳二亚胺 、 N,N-二异丙基乙胺 作用下， 以 二氯甲烷 为溶剂， 反应 12.0h， 以294 mg的产率得到
  参考文献：
  名称：

  Identification of trimannoside-recognizing peptide sequences from a T7 phage display screen using a QCM device

  摘要：

  Here, we report on the identification of trimannoside-recognizing peptide sequences from a T7 phage display screen using a quartz-crystal microbalance (QCM) device. A trimannoside derivative that can form a self-assembled monolayer (SAM) was synthesized and used for immobilization on the gold electrode surface of a QCM sensor chip. After six sets of one-cycle affinity selection, T7 phage particles displaying PSVGLFTH (8-mer) and SVGLGLGFSTVNCF (14-mer) were found to be enriched at a rate of 17/44, 9/44, respectively, suggesting that these peptides specifically recognize trimannoside. Binding checks using the respective single T7 phage and synthetic peptide also confirmed the specific binding of these sequences to the trimannoside-SAM. Subsequent analysis revealed that these sequences correspond to part of the primary amino acid sequence found in many mannose- or hexose- related proteins. Taken together, these results demonstrate the effectiveness of our T7 phage display environment for affinity selection of binding peptides. We anticipate this screening result will also be extremely useful in the development of inhibitors or drug delivery systems targeting polysaccharides as well as further investigations into the function of carbohydrates in vivo. (c) 2008 Elsevier Ltd. All rights reserved.

  DOI：

  10.1016/j.bmc.2008.11.004