p-Nitrophenyl 2,3,4-tri-O-acetyl-6-azido-6-deoxy-alpha-D-galactopyranoside | 1170659-48-9

• 分子结构分类: 有机化合物 - 有机氧化合物
• 英文名称: p-Nitrophenyl 2,3,4-tri-O-acetyl-6-azido-6-deoxy-alpha-D-galactopyranoside
• 英文别名: [(2R,3S,4S,5R,6R)-4,5-diacetyloxy-2-(azidomethyl)-6-(4-nitrophenoxy)oxan-3-yl] acetate
• CAS: 1170659-48-9
• 化学式: C₁₈H₂₀N₄O₁₀
• 分子量: 452.378
• InChiKey: IMSFQWHBQSRJOY-ICUGJSFKSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  2.4
• 重原子数：
  32
• 可旋转键数：
  10
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.5
• 拓扑面积：
  158
• 氢给体数：
  0
• 氢受体数：
  12

反应信息

• 作为反应物：
  描述：

  p-Nitrophenyl 2,3,4-tri-O-acetyl-6-azido-6-deoxy-alpha-D-galactopyranoside 在 甲醇 、 sodium methylate 作用下， 以0.056 g的产率得到4-nitrophenyl 6-azido-6-deoxy-α-D-galactopyranoside
  参考文献：
  名称：

  Activity of Debaryomyces hansenii UFV-1 α-galactosidases against α-d-galactopyranoside derivatives

  摘要：

  alpha-D-Galactopyranosides were synthesized and their inhibitory activities toward the Debatyomyces hansenil UFV-1 extracellular and intracellular alpha-galactosidases were evaluated. Methyl alpha-D-galactopyranoside was the most potent inhibitor compared to the others tested, with K'(i) values of 0.82 and 1.12 mmol L-1, for extracellular and intracellular enzymes, respectively. These results indicate that the presence of a hydroxyl group in the C-6 position of alpha-D-galactopyranoside derivatives is important for the recognition by D. hansenii UFV-1 alpha-galactosidases. (C) 2011 Elsevier Ltd. All rights reserved.

  DOI：

  10.1016/j.carres.2011.01.024
• 作为产物：
  描述：

  在 sodium azide 作用下， 以 N,N-二甲基甲酰胺 为溶剂， 反应 72.0h， 生成 p-Nitrophenyl 2,3,4-tri-O-acetyl-6-azido-6-deoxy-alpha-D-galactopyranoside
  参考文献：
  名称：

  Activity of Debaryomyces hansenii UFV-1 α-galactosidases against α-d-galactopyranoside derivatives

  摘要：

  alpha-D-Galactopyranosides were synthesized and their inhibitory activities toward the Debatyomyces hansenil UFV-1 extracellular and intracellular alpha-galactosidases were evaluated. Methyl alpha-D-galactopyranoside was the most potent inhibitor compared to the others tested, with K'(i) values of 0.82 and 1.12 mmol L-1, for extracellular and intracellular enzymes, respectively. These results indicate that the presence of a hydroxyl group in the C-6 position of alpha-D-galactopyranoside derivatives is important for the recognition by D. hansenii UFV-1 alpha-galactosidases. (C) 2011 Elsevier Ltd. All rights reserved.

  DOI：

  10.1016/j.carres.2011.01.024

文献信息

• Modified carbohydrate processing enzyme
  申请人：Isis Innovation Limited

  公开号：US07572604B2

  公开（公告）日：2009-08-11

  A modified polypeptide having carbohydrate processing enzymatic activity is provided, said polypeptide comprising an amino acid sequence selected from: (a) the amino acid sequence of SEQ ID NO:2 comprising a mutation in at least one of W433, E432 and M439; (b) the amino acid sequence of an enzyme of glycosyl hydrolase family 1, comprising at least one mutation at an amino acid residue equivalent to W433, E432 or M439 of SEQ ID NO:2; and (c) a variant of (a) or (b) having carbohydrate processing enzymatic activity and comprising at least one amino acid mutation at a position equivalent to W433, E432 or M439 of SEQ ID NO:2.

  提供了一种具有碳水化合物加工酶活性的改性多肽，该多肽包括以下氨基酸序列之一：(a) SEQ ID NO: 2的氨基酸序列，其中至少在W433、E432和M439中的一个上发生了突变；(b) 一种酶的氨基酸序列，属于糖基水解酶家族1，其中至少在与SEQ ID NO: 2的W433、E432或M439等价的氨基酸残基上发生了突变；以及(c) 具有碳水化合物加工酶活性的(a)或(b)的变体，其中至少在与SEQ ID NO: 2的W433、E432或M439等价的位置上发生了至少一个氨基酸突变。
• MODIFIED CARBOHYDRATE PROCESSING ENZYME
  申请人：Davis Benjamin G.

  公开号：US20090181444A1

  公开（公告）日：2009-07-16

  A modified polypeptide having carbohydrate processing enzymatic activity is provided, said polypeptide comprising an amino acid sequence selected from: (a) the amino acid sequence of SEQ ID NO:2 comprising a mutation in at least one of W433, E432 and M439; (b) the amino acid sequence of an enzyme of glycosyl hydrolase family 1, comprising at least one mutation at an amino acid residue equivalent to W433, E432 or M439 of SEQ ID NO:2; and (c) a variant of (a) or (b) having carbohydrate processing enzymatic activity and comprising at least one amino acid mutation at a position equivalent to W433, E432 or M439 of SEQ ID NO:2.

  提供一种具有碳水化合物加工酶活性的改性多肽，该多肽包括以下氨基酸序列之一：(a) SEQ ID NO: 2的氨基酸序列，其中至少有一个W433、E432和M439突变；(b) 包括至少一个氨基酸残基等同于SEQ ID NO:2的W433、E432或M439的糖基水解酶家族1酶的氨基酸序列；以及(c) 具有碳水化合物加工酶活性的(a)或(b)的变体，其中包括至少一个氨基酸突变位于等同于SEQ ID NO:2的W433、E432或M439的位置。
• US7572604B2
  申请人：——

  公开号：US7572604B2

  公开（公告）日：2009-08-11