Trimethyl-[(3,7,7-trimethyl-1-bicyclo[4.1.0]heptanyl)oxymethyl]azanium

• 分子结构分类: 有机化合物 - 脂质和类脂质分子 - 异戊烯醇脂质
• 英文名称: Trimethyl-[(3,7,7-trimethyl-1-bicyclo[4.1.0]heptanyl)oxymethyl]azanium
• 英文别名: trimethyl-[(3,7,7-trimethyl-1-bicyclo[4.1.0]heptanyl)oxymethyl]azanium
• 化学式: C14H28NO+
• 分子量: 226.38
• InChiKey: GWDIUSBNDQLQJZ-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  3.2
• 重原子数：
  16
• 可旋转键数：
  3
• 环数：
  2.0
• sp3杂化的碳原子比例：
  1.0
• 拓扑面积：
  9.2
• 氢给体数：
  0
• 氢受体数：
  1

文献信息

• Single cell bar-coding for antibody discovery
  申请人：AbVitro LLC

  公开号：US10119134B2

  公开（公告）日：2018-11-06

  Provided herein are methods and composition for immune repertoire sequencing and single cell barcoding. In some aspects, such methods may comprise steps of: (a) forming a plurality of vessels each comprising a first cell cDNA of a first cell polynucleotide from a single cell and a second cell cDNA of a second cell polynucleotide from the single cell; (b) extending the first cell cDNA that is hybridized to a barcoded polynucleotide comprising a primer binding site sequence and a barcode sequence and a cDNA annealing sequence, or an amplicon thereof; and extending the second cell cDNA that is hybridized to a barcoded polynucleotide comprising a primer binding site sequence and the barcode sequence and a cDNA annealing sequence, or an amplicon thereof; and (c) amplifying the extended first cell cDNA and the extended second cell cDNA with a primer set.

  本文提供了用于免疫复合物测序和单细胞条形码的方法和组合物。在某些方面，此类方法可包括以下步骤：(a) 形成多个容器，每个容器包括来自单细胞的第一细胞多核苷酸的第一细胞 cDNA 和来自单细胞的第二细胞多核苷酸的第二细胞 cDNA；(b) 扩展与条形码多核苷酸杂交的第一细胞 cDNA，该条形码多核苷酸包括引物结合位点序列、条形码序列和 cDNA 退火序列或其扩增子；(c) 用引物组扩增扩展的第一细胞 cDNA 和扩展的第二细胞 cDNA。
• Methods of single-cell barcoding and sequencing
  申请人：AbVitro LLC

  公开号：US10392614B2

  公开（公告）日：2019-08-27

  Provided herein are methods for immune repertoire sequencing and single cell barcoding. In some aspects, such methods may comprise producing a plurality of cDNAs from a plurality of polynucleotides, wherein at least one polynucleotide encodes a heavy and/or light chain, from a plurality of immune cells from a biological sample. Producing a plurality of cDNAs may comprise reverse transcription and template switching, such as to form a plurality of uniquely barcoded cDNAs. Methods described herein further comprise amplifying the plurality of uniquely barcoded cDNAs, thereby forming a library, and sequencing one or more of the sequences of the library.

  本文提供的是用于免疫复合物测序和单细胞条形码的方法。在某些方面，这种方法可包括从生物样本中的多个免疫细胞中，用多个多核苷酸产生多个 cDNA，其中至少有一个多核苷酸编码重链和/或轻链。产生多个 cDNA 可包括反转录和模板转换，如形成多个唯一的条形码 cDNA。本文所述方法还包括扩增多个唯一条形码 cDNA，从而形成文库，并对文库中的一个或多个序列进行测序。
• Targeted sequencing and UID filtering
  申请人：Roche Molecular Systems, Inc.

  公开号：US10421999B2

  公开（公告）日：2019-09-24

  Provided herein are methods, compositions, and kits for targeted sequencing of polynucleotides with high accuracy and low amplification and sequencing errors.

  本文提供的方法、组合物和试剂盒用于对多核苷酸进行高精度、低扩增和测序误差的靶向测序。
• Methods of selecting T cell receptors using affinity oligonucleotide conjugates
  申请人：AbVitro LLC

  公开号：US10393743B2

  公开（公告）日：2019-08-27

  Provided herein are methods and compositions for single cell characterization using affinity-oligonucleotide conjugates, including tetramer-oligonucleotide conjugates (e.g., pMHC-oligonucleotide). In some aspects, such methods may comprise the steps of: forming a vessel comprising an affinity-oligonucleotide conjugate and a T cell; attaching a vessel barcoded polynucleotide or complement thereof to: (a) an oligonucleotide portion of a pMHC-oligonucleotide conjugate, and (b) a T cell receptor (TCR) polynucleotide originating from the T cell; and sequencing the oligonucleotide portion or a complement thereof and the TCR polynucleotide or a complement thereof. Additional methods provided herein include determining the binding affinity of a T cell receptor (TCR) to a pMHC-oligonucleotide. Compositions of the present invention encompass a plurality of vessels, wherein a vessel of the plurality of vessels may comprise a single T cell; and a vessel barcoded polynucleotide, wherein the vessel may further comprise a pMHC-oligonucleotide conjugate.

  本文提供了使用亲和性-寡核苷酸共轭物，包括四聚体-寡核苷酸共轭物（如 pMHC-寡核苷酸）进行单细胞表征的方法和组合物。在某些方面，这种方法可包括以下步骤：形成包含亲和素-寡核苷酸共轭物和 T 细胞的血管；将血管条形码多核苷酸或其补体附着到：(a)pMHC-寡核苷酸共轭物的寡核苷酸部分，和(b)来自T细胞的T细胞受体(TCR)多核苷酸；和对寡核苷酸部分或其补体和TCR多核苷酸或其补体进行测序。本文提供的其他方法包括确定 T 细胞受体（TCR）与 pMHC-寡核苷酸的结合亲和力。本发明的组合物包括多个血管，其中多个血管中的一个血管可包括单个T细胞；以及血管条形码多核苷酸，其中血管可进一步包括pMHC-寡核苷酸共轭物。
• High-throughput nucleotide library sequencing
  申请人：AbVitro, LLC

  公开号：US10590483B2

  公开（公告）日：2020-03-17

  Provided herein are methods and composition for immune repertoire sequencing and single cell barcoding. The methods and compositions can be used to pair any two sequences originating from a single cell, such as heavy and light chain antibody sequences, alpha and beta chain T-cell receptor sequences, or gamma and delta chain T-cell receptor sequences, for antibody and T-cell receptor discovery, disease and immune diagnostics, and low error sequencing.

  本文提供了用于免疫复合物测序和单细胞条形码的方法和组合物。这些方法和组合物可用于配对源自单细胞的任意两个序列，如重链和轻链抗体序列、α链和β链 T 细胞受体序列或γ链和δ链 T 细胞受体序列，以发现抗体和 T 细胞受体、进行疾病和免疫诊断以及低误差测序。