5-acetylamino-2-(4-methoxybenzyl)-2H-pyrazole-3-carboxylic acid methyl ester | 650609-98-6

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 唑类
• 英文名称: 5-acetylamino-2-(4-methoxybenzyl)-2H-pyrazole-3-carboxylic acid methyl ester
• 英文别名: Methyl 3-(acetylamino)-1-[(4-methoxyphenyl)methyl]-1H-pyrazole-5-carboxylate；methyl 5-acetamido-2-[(4-methoxyphenyl)methyl]pyrazole-3-carboxylate
• CAS: 650609-98-6
• 化学式: C₁₅H₁₇N₃O₄
• 分子量: 303.318
• InChiKey: KHOHIVXZERRFAD-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  1.69
• 重原子数：
  22.0
• 可旋转键数：
  5.0
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.27
• 拓扑面积：
  82.45
• 氢给体数：
  1.0
• 氢受体数：
  6.0

反应信息

• 作为反应物：
  描述：

  5-acetylamino-2-(4-methoxybenzyl)-2H-pyrazole-3-carboxylic acid methyl ester 在 lithium hydroxide 、 2-氯-1-甲基吡啶碘化物 、 N,N-二异丙基乙胺 作用下， 以 四氢呋喃 、 甲醇 、 二氯甲烷 为溶剂， 反应 16.0h， 生成 5-acetylamino-2-[4-methoxybenzyl]-2H-pyrazole-3-carbonylamino-2-[4-methoxybenzyl]-2H-pyrazole-3-carbonyl-Nε-(tert-butyloxycarbonyl)-(S)-lysinyl-(S)-valinyl-(S)-phenylalanine methyl ester
  参考文献：
  名称：

  β-Sheet Ligands in Action:  KLVFF Recognition by Aminopyrazole Hybrid Receptors in Water

  摘要：

  Little is known about the precise mechanism of action of beta-sheet ligands, hampered by the notorious solubility problems involved with protein misfolding and amyloid formation. Recently the nucleation site for the pathogenic aggregation of the Alzheimer's peptide was identified as the KLVFF sequence in the central region of Abeta. A combination of two aminopyrazole ligands with di- or tripepticles taken from this key fragment now furnished water-soluble Abeta-specific ligands which allow model investigations in water. A detailed conformational analysis provides experimental evidence for an increased -sheet content induced in the peptide. Strong indications were also found for the peptide backbone recognition via hydrogen bonds plus hydrophobic contributions between aminopyrazole nuclei and Phe residues. The affinity of these new ligands toward the KKLVFF fragment is highly dependent on their sequence and composition from natural and artificial amino acids. Thus, for the first time, detailed insight is gained into the complexation of beta-sheet ligands with model peptices taken directly from Abeta.

  DOI：

  10.1021/ja045558b
• 作为产物：
  描述：

  2-(4-甲氧基苄基)-5-硝基-2H-吡唑-3-羧酸甲酯 在 palladium on activated charcoal 4-二甲氨基吡啶 、 氢气 、 N,N-二异丙基乙胺 作用下， 以 四氢呋喃 、 甲醇 、 二氯甲烷 为溶剂， 反应 20.0h， 生成 5-acetylamino-2-(4-methoxybenzyl)-2H-pyrazole-3-carboxylic acid methyl ester
  参考文献：
  名称：

  Aminopyrazole Oligomers for β-SheetStabilization of Peptides

  摘要：

  本文介绍了利用设计的人工配体稳定δ-片材的一般概念。这些配体有两个主要特征：它们含有酰化的 3-氨基吡唑，具有 DAD 氢键供体和受体模式；它们被合成为低聚物，以增加它们与δ-片构象中的肽的氢键相互作用。二聚氨基吡唑可通过 N1-Boc 保护的氨基吡唑衍生物 1 与几种二氯酸反应，然后用三氟乙酸进行标准脱保护处理而获得。对于低聚物，对新的吡唑氨基酸进行 N1-PMB 保护，然后使用 PyClop 或 Mukaiyama 试剂与肽偶联进行迭代扩展，最终得到目标化合物。随后，所有的保护基团都在最后一步用温三氟乙酸进行脱保护。对两个二聚关键化合物 3b 和 3f 进行了不同温度下的核磁共振、NOESY 实验和 X 射线晶体学研究，以阐明它们在溶液和固体状态下的构象偏好。所有方法得出的结果都是一样的：两种配体都采用具有高度预取向性的扁平构象和正确的 DAD 模式，以便与扩展构象中的肽发生最佳相互作用。用朊病毒蛋白和阿尔茨海默氏症肽 Aβ (1-40) 进行的聚合试验表明，一些二聚体和寡聚体配体在极低浓度下就能产生很好的效果。

  DOI：

  10.1055/s-2003-41031

文献信息

• β-Sheet Ligands in Action:  KLVFF Recognition by Aminopyrazole Hybrid Receptors in Water
  作者：Petra Rzepecki、Thomas Schrader

  DOI：10.1021/ja045558b

  日期：2005.3.9

  Little is known about the precise mechanism of action of beta-sheet ligands, hampered by the notorious solubility problems involved with protein misfolding and amyloid formation. Recently the nucleation site for the pathogenic aggregation of the Alzheimer's peptide was identified as the KLVFF sequence in the central region of Abeta. A combination of two aminopyrazole ligands with di- or tripepticles taken from this key fragment now furnished water-soluble Abeta-specific ligands which allow model investigations in water. A detailed conformational analysis provides experimental evidence for an increased -sheet content induced in the peptide. Strong indications were also found for the peptide backbone recognition via hydrogen bonds plus hydrophobic contributions between aminopyrazole nuclei and Phe residues. The affinity of these new ligands toward the KKLVFF fragment is highly dependent on their sequence and composition from natural and artificial amino acids. Thus, for the first time, detailed insight is gained into the complexation of beta-sheet ligands with model peptices taken directly from Abeta.
• Aminopyrazole Oligomers for β-SheetStabilization of Peptides
  作者：T. Schrader、P. Rzepecki、M. Wehner、O. Molt、R. Zadmard、K. Harms

  DOI：10.1055/s-2003-41031

  日期：——

  A general concept for the stabilization of β-sheetsby designed artificial ligands is introduced. The ligands have twokey features: they contain acylated 3-aminopyrazoles with a DAD hydrogenbond donor and acceptor pattern, and they were synthesized as oligomersin order to multiply their hydrogen bond interactions with peptidesin the β-sheet conformation. Dimeric aminopyrazoles wereaccessible by reaction of the N1-Boc-protectedaminopyrazole derivative 1 with severalacid dichlorides followed by a standard deprotection procedure withtrifluoroacetic acid. For the oligomers, N1-PMBprotection of new pyrazole amino acids followed by an iterativeextension protocol with peptide coupling using PyClop or Mukaiyama’sreagent led to the target compounds. All protecting groups weresubsequently removed in a final deprotection step with warm trifluoroaceticacid. Two dimeric key compounds 3b and 3f were examined by NMR at various temperatures,in NOESY experiments as well as by X-ray crystallography in orderto elucidate their conformational preference in solution and thesolid state. The emerging picture was the same for all methods: bothligands adopt a flat conformation with a high degree of pre-orientationand the correct DAD pattern for optimal interaction with peptidesin their extended conformation. Aggregation assays with the Prionprotein and the Alzheimer’s peptide Aβ (1-40)show highly promising results for some of the dimeric and oligomericligands at very low concentrations.

  本文介绍了利用设计的人工配体稳定δ-片材的一般概念。这些配体有两个主要特征：它们含有酰化的 3-氨基吡唑，具有 DAD 氢键供体和受体模式；它们被合成为低聚物，以增加它们与δ-片构象中的肽的氢键相互作用。二聚氨基吡唑可通过 N1-Boc 保护的氨基吡唑衍生物 1 与几种二氯酸反应，然后用三氟乙酸进行标准脱保护处理而获得。对于低聚物，对新的吡唑氨基酸进行 N1-PMB 保护，然后使用 PyClop 或 Mukaiyama 试剂与肽偶联进行迭代扩展，最终得到目标化合物。随后，所有的保护基团都在最后一步用温三氟乙酸进行脱保护。对两个二聚关键化合物 3b 和 3f 进行了不同温度下的核磁共振、NOESY 实验和 X 射线晶体学研究，以阐明它们在溶液和固体状态下的构象偏好。所有方法得出的结果都是一样的：两种配体都采用具有高度预取向性的扁平构象和正确的 DAD 模式，以便与扩展构象中的肽发生最佳相互作用。用朊病毒蛋白和阿尔茨海默氏症肽 Aβ (1-40) 进行的聚合试验表明，一些二聚体和寡聚体配体在极低浓度下就能产生很好的效果。