Novel compounds comprising a C—C double bond substituted at one carbon with two oxygen or sulfur atom-containing groups are disclosed. The compounds are useful in methods and compositions for generating chemiluminescence by reaction with a peroxidase enzyme and a peroxide. The chemiluminescence thus produced can be used as a detectable signal in assays for peroxidase enzymes and in assays employing enzyme-labeled specific binding pairs.
本发明揭示了一种包含一条C-C双键,其中一个碳原子被两个含氧或
硫原子的基团取代的新化合物。这些化合物可用于通过与
过氧化物酶和过氧化物反应产生
化学发光的方法和组合物中。因此产生的
化学发光可以用作检测
过氧化物酶的测定中的可检测信号,并且可以用于利用酶标记的特异性结合对的测定中。